Probable Histone-Lysine N-Methyltransferase (ASH1L) ELISA Kit for Human

Name: Human ASH1L ELISA Kit; Probable Histone-Lysine N-Methyltransferase; MyBioSource
Brand: MyBioSource
SKU: MBS283034
Price: 495 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS283034

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate ASH1L in Human serum, plasma. An antibody specific for ASH1L has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ASH1L present is… More details

$495 / 48 Tests +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

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Product Overview

Online Datasheet in PDF

Product Name

Probable histone-lysine N-methyltransferase (ASH1L), ELISA Kit

Full Product Name

Human Probable histone-lysine N-methyltransferase (ASH1L) ELISA Kit

Product Gene Name

ASH1L elisa kit

Species Reactivity

Human

Assay Type

Quantitative Sandwich

Samples

Serum, plasma and other biological fluids

Detection Range

31.25 pg/mL - 2000 pg/mL

Sensitivity

15.6 pg/mL (mean of 6 independent assays)

Specificity

This assay has high sensitivity and excellent specificity for detection of Human ASH1L. No significant cross-reactivity or interference between Human ASH1L and analogues was observed.

Preparation and Storage

Store at 2-8 degree C.

Inter-assay Precision

Inter-assay Precision (Precision between assays) Three samples of known concentration were tested in forty separate assays to assess inter-assay precision. CV (%) = SD/meanX100. Inter-Assay: CV<12%

Intra-assay Precision

Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision. Intra-Assay: CV<8%

Molecular Weight

332,211 Da

OMIM

AF257305 mRNA

3D Structure

ModBase 3D Structure for Q9NR48

ISO Certification

Manufactured in an ISO 9001:2015 Certified Laboratory.

Other Notes

Small volumes of ASH1L elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for ASH1L purchase

MBS283034 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Probable histone-lysine N-methyltransferase (ASH1L) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing ASH1L. The ELISA analytical biochemical technique of the MBS283034 kit is based on ASH1L antibody-ASH1L antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect ASH1L antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, ASH1L. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for ASH1L. It may not necessarily be applicable to this product.

NCBI GI #

110349788

NCBI GeneID

55870

NCBI Accession #

NP_060959.2

UniProt Primary Accession #

Q9NR48

UniProt Secondary Accession #

Q59GP1; Q5T714; Q5T715; Q9P2C7

UniProt Related Accession #

Q9NR48

GenBank Nucleotide #

NM_018489.2

NCBI Official Full Name

histone-lysine N-methyltransferase ASH1L

NCBI Official Synonym Full Names

ASH1 like histone lysine methyltransferase

NCBI Official Symbol

ASH1L

NCBI Official Synonym Symbols

ASH1; KMT2H; MRD52; ASH1L1

UniProt Protein Name

Histone-lysine N-methyltransferase ASH1L

UniProt Synonym Protein Names

ASH1-like protein; huASH1; Absent small and homeotic disks protein 1 homolog; Lysine N-methyltransferase 2H

Protein Family

Histone-lysine N-methyltransferase

UniProt Gene Name

ASH1L

UniProt Synonym Gene Names

KIAA1420; KMT2H; huASH1

NCBI Protein Information

histone-lysine N-methyltransferase ASH1L

NCBI Summary for ASH1L

This gene encodes a member of the trithorax group of transcriptional activators. The protein contains four AT hooks, a SET domain, a PHD-finger motif, and a bromodomain. It is localized to many small speckles in the nucleus, and also to cell-cell tight junctions. [provided by RefSeq, Jul 2008]

UniProt Comments for ASH1L

ASH1L: a protein methyltransferase that specifically methylates K36 of histone H3 (H3K36me). A member of the trithorax group (trxG) of transcriptional activators that is required for proper expression of Hox genes and is preferentially expressed in haematopoietic stem cells. Widely expressed, with high levels in brain, heart and kidney. The protein contains four AT hooks, a SET domain, a PHD-finger motif, and a bromodomain. It is localized to many small speckles in the nucleus, and also to cell-cell tight junctions.

Protein type: Amino Acid Metabolism - lysine degradation; Cell adhesion; EC 2.1.1.43; Methyltransferase; Methyltransferase, protein lysine

Chromosomal Location of Human Ortholog: 1q22

Cellular Component: bicellular tight junction; chromosome; extracellular exosome; Golgi apparatus; nucleoplasm; nucleus

Molecular Function: chromatin binding; DNA binding; histone methyltransferase activity (H3-K36 specific); histone methyltransferase activity (H3-K4 specific); histone-lysine N-methyltransferase activity; metal ion binding

Biological Process: cell-cell signaling; decidualization; DNA packaging; histone H3-K4 methylation; interleukin-6 production; negative regulation of acute inflammatory response; negative regulation of I-kappaB kinase/NF-kappaB signaling; negative regulation of MAPKKK cascade; positive regulation of transcription from RNA polymerase II promoter; post-embryonic development; single fertilization; skeletal system development; transcription from RNA polymerase II promoter

Disease: Mental Retardation, Autosomal Dominant 52

Research Articles on ASH1L

1. suppresses matrix metalloproteinase through mitogen-activated protein kinase signaling pathway in pulpitis

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with ASH1L elisa kit

Products by Pathway	Pathway Diagram
Lysine Degradation Pathway antibodies	Lysine Degradation Pathway Diagram
Lysine Degradation Pathway antibodies	Lysine Degradation Pathway Diagram
Tight Junction Pathway antibodies	Tight Junction Pathway Diagram
Tight Junction Pathway antibodies	Tight Junction Pathway Diagram

Diseases associated with ASH1L elisa kit

Disease Name	Pubmed Publications
Neoplasms Antibodies	>7 publications with ASH1L and Neoplasms
Lung Neoplasms Antibodies	>3 publications with ASH1L and Lung Neoplasms
Lung Diseases Antibodies	>3 publications with ASH1L and Lung Diseases
Adenocarcinoma Antibodies	>3 publications with ASH1L and Adenocarcinoma
Adenoma Antibodies	>3 publications with ASH1L and Adenoma
Brain Diseases Antibodies	>2 publications with ASH1L and Brain Diseases
Neoplasms, Experimental Antibodies	>1 publications with ASH1L and Neoplasms, Experimental
Liver Diseases Antibodies	>1 publications with ASH1L and Liver Diseases
Hyperplasia Antibodies	>1 publications with ASH1L and Hyperplasia
Cell Transformation, Neoplastic Antibodies	>1 publications with ASH1L and Cell Transformation, Neoplastic

Organs/Tissues associated with ASH1L elisa kit

Organ/Tissue Name	Pubmed Publications
Lung Antibodies	>3 publications with ASH1L and Lung
Pineal Gland Antibodies	>1 publications with ASH1L and Pineal Gland
Bone Antibodies	>1 publications with ASH1L and Bone
Eye Antibodies	>1 publications with ASH1L and Eye
Adrenal Gland Antibodies	>1 publications with ASH1L and Adrenal Gland
Embryonic Tissue Antibodies	>1 publications with ASH1L and Embryonic Tissue
Blood Antibodies	>1 publications with ASH1L and Blood
Liver Antibodies	>1 publications with ASH1L and Liver
Brain Antibodies	>1 publications with ASH1L and Brain