Putative Pre-Mrna-Splicing Factor ATP-Dependent RNA Helicase DHX32 (DHX32) ELISA Kit for Human

Name: Human DHX32 ELISA Kit; MyBioSource
Brand: MyBioSource
SKU: MBS283280
Price: 495 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS283280

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate DHX32 in samples. An antibody specific for DHX32 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyDHX32 present is bound by the… More details

$495 / 48 Tests +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Request Current Manual

Product Overview

Online Datasheet in PDF

Product Name

Putative pre-mRNA-splicing factor ATP-dependent RNA helicase DHX32, ELISA Kit

Full Product Name

Human Putative pre-mRNA-splicing factor ATP-dependent RNA helicase DHX32 (DHX32) ELISA Kit

Product Gene Name

DHX32 elisa kit

Species Reactivity

Human

Assay Type

Sandwich

Samples

Serum, Plasma, Other biological fluids

Specificity

This assay has high sensitivity and excellent specificity for detection of Human DHX32. No significant cross-reactivity or interference between Human DHX32 and analogues was observed.

Preparation and Storage

Store at 2-8 degree C.

Molecular Weight

75,088 Da

OMIM

AF427340 mRNA

3D Structure

ModBase 3D Structure for Q7L7V1

Sample Volume

1-200 uL

Precision

Intra-assay Precision (Precision within an assay)
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%) = SD/meanX100
Intra-Assay: CV
Inter-Assay: CV

Detection Wavelength

450 nm

ISO Certification

Manufactured in an ISO 9001:2015 Certified Laboratory.

Other Notes

Small volumes of DHX32 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for DHX32 purchase

MBS283280 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Putative pre-mRNA-splicing factor ATP-dependent RNA helicase DHX32, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing DHX32. The ELISA analytical biochemical technique of the MBS283280 kit is based on DHX32 antibody-DHX32 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect DHX32 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, DHX32. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for DHX32. It may not necessarily be applicable to this product.

NCBI GI #

20336300

NCBI GeneID

55760

NCBI Accession #

NP_060650.2

UniProt Primary Accession #

Q7L7V1

UniProt Secondary Accession #

Q49AG5; Q5T3L0; Q5T3L5; Q96NY1; Q9BUN0; Q9H769; Q9NSL5; Q9NV74; Q9NVJ7; A8MSV2; D3DRF9

UniProt Related Accession #

Q7L7V1

GenBank Nucleotide #

NM_018180.2

NCBI Official Full Name

putative pre-mRNA-splicing factor ATP-dependent RNA helicase DHX32

NCBI Official Synonym Full Names

DEAH-box helicase 32 (putative)

NCBI Official Symbol

DHX32

NCBI Official Synonym Symbols

DDX32; DHLP1

UniProt Protein Name

Putative pre-mRNA-splicing factor ATP-dependent RNA helicase DHX32

UniProt Synonym Protein Names

DEAD/H box 32; DEAD/H helicase-like protein 1; DHLP1; DEAH box protein 32; HuDDX32

Protein Family

Putative pre-mRNA-splicing factor ATP-dependent RNA helicase

UniProt Gene Name

DHX32

UniProt Synonym Gene Names

DDX32; DHLP1

NCBI Protein Information

putative pre-mRNA-splicing factor ATP-dependent RNA helicase DHX32

NCBI Summary for DHX32

DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this DEAD box protein family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a member of this family. The function of this member has not been determined. Alternative splicing of this gene generates 2 transcript variants, but the full length nature of one of the variants has not been defined. [provided by RefSeq, Jul 2008]

UniProt Comments for DHX32

DHX32: DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this DEAD box protein family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a member of this family. The function of this member has not been determined. Alternative splicing of this gene generates 2 transcript variants, but the full length nature of one of the variants has not been defined. [provided by RefSeq, Jul 2008]

Protein type: EC 3.6.4.13; Helicase

Chromosomal Location of Human Ortholog: 10q26.2

Cellular Component: cytoplasm; mitochondrion; spliceosomal complex

Molecular Function: ATP binding; ATP-dependent RNA helicase activity; protein binding; RNA binding

Biological Process: mRNA splicing, via spliceosome

Research Articles on DHX32

1. DHX32 may play an important role in the development of colorectal cancer and could serve as a novel biomarker for colorectal cancer after additional investigation.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Diseases associated with DHX32 elisa kit

Disease Name	Pubmed Publications
Lymphoma Antibodies	>3 publications with DHX32 and Lymphoma
Immune System Diseases Antibodies	>3 publications with DHX32 and Immune System Diseases

Organs/Tissues associated with DHX32 elisa kit

Organ/Tissue Name	Pubmed Publications
Liver Antibodies	>1 publications with DHX32 and Liver
Kidney Antibodies	>1 publications with DHX32 and Kidney