Proteinase-Antineutrophil Cytoplasmic (PR3-ANCA) ELISA Kit for Human

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate PR3-ANCA in samples. An antibody specific for PR3-ANCA has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPR3-ANCA present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PR3-ANCA is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PR3-ANCA bound in the initial step. The color development is stopped and the intensity of the color is measured.

Small volumes of PR3-ANCA elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS283296 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Proteinase-antineutrophil cytoplasmic antibody (PR3-ANCA) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing PR3-ANCA. The ELISA analytical biochemical technique of the MBS283296 kit is based on PR3-ANCA antibody-PR3-ANCA antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect PR3-ANCA antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, PR3-ANCA. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).