Caspase Recruitment Domain-Containing Protein 11 (CARD11) ELISA Kit for Mouse

Name: Mouse CARD11 ELISA Kit; Caspase Recruitment Domain-Containing Protein 11; MyBioSource
Brand: MyBioSource
SKU: MBS283308
Price: 495 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS283308

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate CARD11 in samples. An antibody specific for CARD11 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyCARD11 present is bound by… More details

$495 / 48 Tests +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

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Product Overview

Online Datasheet in PDF

Product Name

Caspase recruitment domain-containing protein 11 (CARD11), ELISA Kit

Full Product Name

Mouse Caspase recruitment domain-containing protein 11 (CARD11) ELISA Kit

Product Gene Name

CARD11 elisa kit

Species Reactivity

Mouse

Assay Type

Sandwich

Samples

Serum, Plasma, Other biological fluids

Specificity

This assay has high sensitivity and excellent specificity for detection of Mouse CARD11. No significant cross-reactivity or interference between Mouse CARD11 and analogues was observed.

Preparation and Storage

Store at 2-8 degree C.

Molecular Weight

133,468 Da

3D Structure

ModBase 3D Structure for Q8CIS0

Sample Volume

1-200 uL

Precision

Intra-assay Precision (Precision within an assay)
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%) = SD/meanX100
Intra-Assay: CV
Inter-Assay: CV

Detection Wavelength

450 nm

ISO Certification

Manufactured in an ISO 9001:2015 Certified Laboratory.

Other Notes

Small volumes of CARD11 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CARD11 purchase

MBS283308 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Caspase recruitment domain-containing protein 11 (CARD11) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing CARD11. The ELISA analytical biochemical technique of the MBS283308 kit is based on CARD11 antibody-CARD11 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect CARD11 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, CARD11. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for CARD11. It may not necessarily be applicable to this product.

NCBI GI #

161086929

NCBI GeneID

108723

NCBI Accession #

NP_780571.2

UniProt Primary Accession #

Q8CIS0

UniProt Secondary Accession #

Q4VA14; Q6KAS3; Q8BYV0

UniProt Related Accession #

Q8CIS0

GenBank Nucleotide #

NM_175362.2

NCBI Official Full Name

caspase recruitment domain-containing protein 11

NCBI Official Synonym Full Names

caspase recruitment domain family, member 11

NCBI Official Symbol

Card11

NCBI Official Synonym Symbols

BIMP3; CARMA1; 0610008L17Rik; 2410011D02Rik

UniProt Protein Name

Caspase recruitment domain-containing protein 11

Protein Family

Caspase recruitment domain-containing protein

UniProt Gene Name

Card11

NCBI Protein Information

caspase recruitment domain-containing protein 11

UniProt Comments for CARD11

CARD11: a CARD (caspase recruitment domain) protein that belongs to the membrane-associated guanylate kinase (MAGUK) family. MAGUK proteins function as molecular scaffolds for the assembly of multiprotein complexes at specialized regions of the plasma membrane. Contains an N-terminal CARD domain, a central coiled-coil domain, and a C-terminal region containing a PDZ, an SH3, and a GUK. Its CARD domain associates with the CARD domain of BCL10. BCL10 activates NF-kappaB through the IkappaB kinase complex in response to upstream stimuli. Apparently activates NF-kappaB and induce the phosphorylation of BCL10. Detected in adult peripheral blood leukocytes, thymus, spleen and liver. Also found in promyelocytic leukemia HL-60 cells, chronic myelogenous leukemia K562 cells, Burkitt's lymphoma Raji cells and colorectal adenocarcinoma SW480 cells.

Protein type: Adaptor/scaffold

Chromosomal Location of Human Ortholog: 5|5 G2

Cellular Component: CBM complex; cytoplasm; extracellular exosome; immunological synapse; membrane; membrane raft; plasma membrane; T cell receptor complex

Molecular Function: CARD domain binding; protein binding

Biological Process: B cell differentiation; B cell proliferation; homeostasis of number of cells; I-kappaB kinase/NF-kappaB signaling; immunoglobulin production; interleukin-2 secretion; lymphocyte activation; positive regulation of B cell proliferation; positive regulation of cytokine production; positive regulation of I-kappaB kinase/NF-kappaB signaling; positive regulation of interleukin-2 biosynthetic process; positive regulation of NF-kappaB transcription factor activity; positive regulation of T cell activation; positive regulation of T cell proliferation; regulation of apoptosis; regulation of B cell differentiation; regulation of immune response; regulation of T cell differentiation; signal transduction; T cell activation; T cell costimulation; thymic T cell selection; TORC1 signaling

Research Articles on CARD11

1. synergistic activity of Card11 mutant and Bcl6 in the development of diffuse large B-cell lymphoma in a mouse model

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with CARD11 elisa kit

Products by Pathway	Pathway Diagram
Adaptive Immune System Pathway antibodies	Adaptive Immune System Pathway Diagram
B Cell Receptor Signaling Pathway antibodies	B Cell Receptor Signaling Pathway Diagram
B Cell Receptor Signaling Pathway antibodies	B Cell Receptor Signaling Pathway Diagram
B Cell Receptor Signaling Pathway antibodies	B Cell Receptor Signaling Pathway Diagram
Downstream TCR Signaling Pathway antibodies	Downstream TCR Signaling Pathway Diagram
Immune System Pathway antibodies	Immune System Pathway Diagram
NF-kappa B Signaling Pathway antibodies	NF-kappa B Signaling Pathway Diagram
T Cell Receptor Signaling Pathway antibodies	T Cell Receptor Signaling Pathway Diagram
T Cell Receptor Signaling Pathway antibodies	T Cell Receptor Signaling Pathway Diagram
T Cell Receptor Signaling Pathway antibodies	T Cell Receptor Signaling Pathway Diagram

Diseases associated with CARD11 elisa kit

Disease Name	Pubmed Publications
Lymphoma Antibodies	>46 publications with CARD11 and Lymphoma
Neoplasms Antibodies	>38 publications with CARD11 and Neoplasms
Lymphoma, T-Cell Antibodies	>14 publications with CARD11 and Lymphoma, T-Cell
Leukemia Antibodies	>6 publications with CARD11 and Leukemia
Inflammation Antibodies	>6 publications with CARD11 and Inflammation
Chromosome Aberrations Antibodies	>4 publications with CARD11 and Chromosome Aberrations
Central Nervous System Neoplasms Antibodies	>3 publications with CARD11 and Central Nervous System Neoplasms
Neoplasms, Experimental Antibodies	>2 publications with CARD11 and Neoplasms, Experimental
Cell Transformation, Neoplastic Antibodies	>2 publications with CARD11 and Cell Transformation, Neoplastic
Leukemia, Myeloid, Acute Antibodies	>1 publications with CARD11 and Leukemia, Myeloid, Acute

Organs/Tissues associated with CARD11 elisa kit

Organ/Tissue Name	Pubmed Publications
Thymus Antibodies	>4 publications with CARD11 and Thymus
Bone Marrow Antibodies	>3 publications with CARD11 and Bone Marrow
Spleen Antibodies	>3 publications with CARD11 and Spleen
Kidney Antibodies	>1 publications with CARD11 and Kidney