Pre-Mrna-Splicing Factor ATP-Dependent RNA Helicase PRP16 (DHX38) ELISA Kit for Human

Name: Human DHX38 ELISA Kit; MyBioSource
Brand: MyBioSource
SKU: MBS283365
Price: 495 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS283365

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate DHX38 in samples. An antibody specific for DHX38 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyDHX38 present is bound by the… More details

$495 / 48 Tests +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

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Product Overview

Online Datasheet in PDF

Product Name

Pre-mRNA-splicing factor ATP-dependent RNA helicase PRP16 (DHX38), ELISA Kit

Full Product Name

Human Pre-mRNA-splicing factor ATP-dependent RNA helicase PRP16 (DHX38) ELISA Kit

Product Gene Name

DHX38 elisa kit

Species Reactivity

Human

Assay Type

Sandwich

Samples

Serum, Plasma, Other biological fluids

Specificity

This assay has high sensitivity and excellent specificity for detection of Human DHX38. No significant cross-reactivity or interference between Human DHX38 and analogues was observed.

Preparation and Storage

Store at 2-8 degree C.

Molecular Weight

60,743 Da

OMIM

AF038391 mRNA

3D Structure

ModBase 3D Structure for Q92620

Sample Volume

1-200 uL

Precision

Intra-assay Precision (Precision within an assay)
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%) = SD/meanX100
Intra-Assay: CV
Inter-Assay: CV

Detection Wavelength

450 nm

ISO Certification

Manufactured in an ISO 9001:2015 Certified Laboratory.

Other Notes

Small volumes of DHX38 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for DHX38 purchase

MBS283365 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Pre-mRNA-splicing factor ATP-dependent RNA helicase PRP16 (DHX38) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing DHX38. The ELISA analytical biochemical technique of the MBS283365 kit is based on DHX38 antibody-DHX38 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect DHX38 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, DHX38. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for DHX38. It may not necessarily be applicable to this product.

NCBI GI #

17999539

NCBI GeneID

9785

NCBI Accession #

NP_054722.2

UniProt Primary Accession #

Q92620

UniProt Secondary Accession #

O75212; Q96HN7; B4DVG8; D3DWS7

UniProt Related Accession #

Q92620

GenBank Nucleotide #

NM_014003.3

NCBI Official Full Name

pre-mRNA-splicing factor ATP-dependent RNA helicase PRP16

NCBI Official Synonym Full Names

DEAH-box helicase 38

NCBI Official Symbol

DHX38

NCBI Official Synonym Symbols

DDX38; PRP16; PRPF16

UniProt Protein Name

Pre-mRNA-splicing factor ATP-dependent RNA helicase PRP16

UniProt Synonym Protein Names

ATP-dependent RNA helicase DHX38; DEAH box protein 38

Protein Family

Pre-mRNA-splicing factor ATP-dependent RNA helicase

UniProt Gene Name

DHX38

UniProt Synonym Gene Names

DDX38; KIAA0224; PRP16

NCBI Protein Information

pre-mRNA-splicing factor ATP-dependent RNA helicase PRP16

NCBI Summary for DHX38

DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. The protein encoded by this gene is a member of the DEAD/H box family of splicing factors. This protein resembles yeast Prp16 more closely than other DEAD/H family members. It is an ATPase and essential for the catalytic step II in pre-mRNA splicing process. [provided by RefSeq, Jul 2008]

UniProt Comments for DHX38

DHX38: Probable ATP-binding RNA helicase involved in pre-mRNA splicing. Belongs to the DEAD box helicase family. DEAH subfamily. PRP16 sub-subfamily.

Protein type: EC 3.6.4.13; Helicase; RNA processing; RNA splicing; RNA-binding; Spliceosome

Chromosomal Location of Human Ortholog: 16q22.2

Cellular Component: cytoplasm; membrane; nucleoplasm; nucleus

Molecular Function: ATP binding; ATP-dependent RNA helicase activity; protein binding; RNA binding

Biological Process: mRNA 3'-end processing; mRNA export from nucleus; mRNA splicing, via spliceosome; RNA export from nucleus; termination of RNA polymerase II transcription

Research Articles on DHX38

1. DHX38 is the first pre-mRNA splicing gene that is putatively associated with autosomal-recessive inherited retinitis pigmentosa.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with DHX38 elisa kit

Reference Product	PubMed Publications
CDC40 elisa kit	>1 publications with DHX38 and CDC40
PRPF8 elisa kit	>1 publications with DHX38 and PRPF8
GPKOW elisa kit	>1 publications with DHX38 and GPKOW

Pathways associated with DHX38 elisa kit

Products by Pathway	Pathway Diagram
Cleavage Of Growing Transcript In The Termination Region Pathway antibodies	Cleavage Of Growing Transcript In The Termination Region Pathway Diagram
Gene Expression Pathway antibodies	Gene Expression Pathway Diagram
Processing Of Capped Intron-Containing Pre-mRNA Pathway antibodies	Processing Of Capped Intron-Containing Pre-mRNA Pathway Diagram
RNA Polymerase II Transcription Pathway antibodies	RNA Polymerase II Transcription Pathway Diagram
RNA Polymerase II Transcription Termination Pathway antibodies	RNA Polymerase II Transcription Termination Pathway Diagram
Spliceosome Pathway antibodies	Spliceosome Pathway Diagram
Spliceosome Pathway antibodies	Spliceosome Pathway Diagram
Transport Of Mature Transcript To Cytoplasm Pathway antibodies	Transport Of Mature Transcript To Cytoplasm Pathway Diagram
Transport Of Mature MRNA Derived From An Intron-Containing Transcript Pathway antibodies	Transport Of Mature MRNA Derived From An Intron-Containing Transcript Pathway Diagram
MRNA 3'-end Processing Pathway antibodies	MRNA 3'-end Processing Pathway Diagram

Diseases associated with DHX38 elisa kit

Disease Name	Pubmed Publications
Weight Gain Antibodies	>1 publications with DHX38 and Weight Gain