Zinc Finger E-Box-Binding Homeobox 2 (ZEB2) ELISA Kit for Mouse

Name: Mouse ZEB2 ELISA Kit; Zinc Finger E-Box-Binding Homeobox 2; MyBioSource
Brand: MyBioSource
SKU: MBS283408
Price: 495 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS283408

TCF8 encodes a human zinc finger transcription factor that represses T-lymphocyte-specific IL2 gene expression by binding to a negative regulatory domain 100 nucleotides 5-prime of the IL2 transcription start site.Delta-Ef1 selectively transactivated the… More details

$495 / 48 Tests +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

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Product Overview

Online Datasheet in PDF

Product Name

Zinc finger E-box-binding homeobox 2 (ZEB2), ELISA Kit

Full Product Name

Mouse Zinc finger E-box-binding homeobox 2 (ZEB2) ELISA Kit

Product Gene Name

ZEB2 elisa kit

Species Reactivity

Mouse

Assay Type

Sandwich

Samples

Serum, Plasma, Other biological fluids

Specificity

This assay has high sensitivity and excellent specificity for detection of Mouse ZEB2. No significant cross-reactivity or interference between Mouse ZEB2 and analogues was observed.

Preparation and Storage

Store at 2-8 degree C.

Molecular Weight

136,615 Da

3D Structure

ModBase 3D Structure for Q9R0G7

Sample Volume

1-200 uL

Precision

Intra-assay Precision (Precision within an assay)
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%) = SD/meanX100
Intra-Assay: CV
Inter-Assay: CV

Detection Wavelength

450 nm

ISO Certification

Manufactured in an ISO 9001:2015 Certified Laboratory.

Other Notes

Small volumes of ZEB2 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for ZEB2 purchase

MBS283408 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Zinc finger E-box-binding homeobox 2 (ZEB2) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing ZEB2. The ELISA analytical biochemical technique of the MBS283408 kit is based on ZEB2 antibody-ZEB2 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect ZEB2 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, ZEB2. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for ZEB2. It may not necessarily be applicable to this product.

NCBI GI #

575501612

NCBI GeneID

24136

NCBI Accession #

NP_001276450.1

UniProt Primary Accession #

Q9R0G7

UniProt Secondary Accession #

Q6P9M5

UniProt Related Accession #

Q9R0G7

GenBank Nucleotide #

NM_001289521.1

NCBI Official Full Name

zinc finger E-box-binding homeobox 2

NCBI Official Synonym Full Names

zinc finger E-box binding homeobox 2

NCBI Official Symbol

Zeb2

NCBI Official Synonym Symbols

SIP1; Zfx1b; Zfhx1b; Zfxh1b; 9130203F04Rik; D130016B08Rik

UniProt Protein Name

Zinc finger E-box-binding homeobox 2

UniProt Synonym Protein Names

Smad-interacting protein 1; Zinc finger homeobox protein 1b

Protein Family

Zinc finger E-box-binding homeobox

UniProt Gene Name

Zeb2

UniProt Synonym Gene Names

Sip1; Zfhx1b; Zfx1b

NCBI Protein Information

zinc finger E-box-binding homeobox 2

UniProt Comments for ZEB2

ZEB2: Transcriptional inhibitor that binds to DNA sequence 5'- CACCT-3' in different promoters. Represses transcription of E- cadherin. Defects in ZEB2 are the cause of Mowat-Wilson syndrome (MWIS); also known as Hirschsprung disease-mental retardation syndrome. A complex developmental disorder characterized by mental retardation, delayed motor development, epilepsy, microcephaly and a wide spectrum of clinically heterogeneous features suggestive of neurocristopathies at the cephalic, cardiac, and vagal levels. Some patients manifest Hirschsprung disease. Affected patients show an easily recognizable facial appearance with deep set eyes and hypertelorism, medially divergent, broad eyebrows, prominent columella, pointed chin and uplifted, notched ear lobes. Belongs to the delta-EF1/ZFH-1 C2H2-type zinc-finger family.

Protein type: C2H2-type zinc finger protein; DNA-binding; Motility/polarity/chemotaxis; Transcription, coactivator/corepressor

Chromosomal Location of Human Ortholog: 2|2 B

Cellular Component: cytosol; nuclear chromatin; nucleolus; nucleus

Molecular Function: DNA binding; metal ion binding; nucleic acid binding; sequence-specific DNA binding

Biological Process: cell proliferation in forebrain; central nervous system development; collateral sprouting; corpus callosum morphogenesis; corticospinal tract morphogenesis; embryonic morphogenesis; hippocampus development; negative regulation of transcription from RNA polymerase II promoter; neural crest cell migration; neural tube closure; pigmentation during development; positive regulation of axonogenesis; positive regulation of JUN kinase activity; positive regulation of melanin biosynthetic process; positive regulation of melanocyte differentiation; positive regulation of transcription from RNA polymerase II promoter; positive regulation of Wnt receptor signaling pathway; regulation of transcription, DNA-templated; somitogenesis; transcription, DNA-dependent

Research Articles on ZEB2

1. Zeb2 is critical in embryonic stem cells for exit from the epiblast state.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with ZEB2 elisa kit

Reference Product	PubMed Publications
Smad1 elisa kit	>1 publications with ZEB2 and Smad1

Pathways associated with ZEB2 elisa kit

Products by Pathway	Pathway Diagram
TGF Beta Signaling Pathway antibodies	TGF Beta Signaling Pathway Diagram
TGF-beta Receptor Signaling Pathway antibodies	TGF-beta Receptor Signaling Pathway Diagram

Diseases associated with ZEB2 elisa kit

Disease Name	Pubmed Publications
Neoplasms Antibodies	>103 publications with ZEB2 and Neoplasms
Mowat-Wilson syndrome Antibodies	>41 publications with ZEB2 and Mowat-Wilson syndrome
Mental Disorders Antibodies	>32 publications with ZEB2 and Mental Disorders
Neoplasm Invasiveness Antibodies	>30 publications with ZEB2 and Neoplasm Invasiveness
Nervous System Malformations Antibodies	>24 publications with ZEB2 and Nervous System Malformations
Neoplasm Metastasis Antibodies	>24 publications with ZEB2 and Neoplasm Metastasis
Breast Neoplasms Antibodies	>21 publications with ZEB2 and Breast Neoplasms
Skin Diseases Antibodies	>16 publications with ZEB2 and Skin Diseases
Cell Transformation, Neoplastic Antibodies	>14 publications with ZEB2 and Cell Transformation, Neoplastic
Heart Defects, Congenital Antibodies	>14 publications with ZEB2 and Heart Defects, Congenital

Organs/Tissues associated with ZEB2 elisa kit

Organ/Tissue Name	Pubmed Publications
Embryonic Tissue Antibodies	>22 publications with ZEB2 and Embryonic Tissue
Heart Antibodies	>21 publications with ZEB2 and Heart
Kidney Antibodies	>18 publications with ZEB2 and Kidney
Lung Antibodies	>12 publications with ZEB2 and Lung
Blood Antibodies	>11 publications with ZEB2 and Blood
Bone Antibodies	>11 publications with ZEB2 and Bone
Brain Antibodies	>11 publications with ZEB2 and Brain
Eye Antibodies	>10 publications with ZEB2 and Eye
Muscle Antibodies	>7 publications with ZEB2 and Muscle
Mammary Gland Antibodies	>6 publications with ZEB2 and Mammary Gland