Soluble Protein-100b (S-100B) ELISA Kit for Rat

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate S-100B in samples. An antibody specific for S-100B has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyS-100B present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for S-100B is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of S-100B bound in the initial step. The color development is stopped and the intensity of the color is measured.

Small volumes of S-100B elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS283466 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Soluble protein-100B (S-100B) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing S-100B. The ELISA analytical biochemical technique of the MBS283466 kit is based on S-100B antibody-S-100B antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect S-100B antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, S-100B. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).