Protein Yippee-Like 2 (YPEL2) ELISA Kit for Human

YPEL2 gene contains 5 exons and spans about 70 kb. The deduced 119-amino acid protein shares 100% amino acid identity with mouse and monkey Ypel2 and 45% identity with Drosophila Yippee. YPEL2 shares 85.0 to 96.6% amino acid identity with YPEL1, YPEL3 , and YPEL4. All YPEL proteins contain an 86-amino acid YPEL consensus sequence. PCR analysis detected YPEL2 expression in adult heart, kidney, lung, pancreas, placenta, skeletal muscle, leukocytes, prostate, spleen, and testis, and in fetal brain, heart, kidney, liver, lung, skeletal muscle, and spleen. Immunofluorescent staining of COS-7 green monkey kidney cells localized monkey Ypel2 to the centrosome and nucleolus during interphase and at several punctate structures around the mitotic apparatus during the mitotic phase.

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate YPEL2 in samples. An antibody specific for YPEL2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyYPEL2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for YPEL2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of YPEL2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Small volumes of YPEL2 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS283503 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Protein yippee-like 2 (YPEL2) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing YPEL2. The ELISA analytical biochemical technique of the MBS283503 kit is based on YPEL2 antibody-YPEL2 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect YPEL2 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, YPEL2. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).