Probable E3 Ubiquitin-Protein Ligase Makorin-2 (MKRN2) ELISA Kit for Human

Name: Human MKRN2 ELISA Kit; Probable E3 Ubiquitin-Protein Ligase Makorin-2; MyBioSource
Brand: MyBioSource
SKU: MBS283514
Price: 495 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS283514

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate MKRN2 in samples. An antibody specific for MKRN2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMKRN2 present is bound by the… More details

$495 / 48 Tests +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

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Product Overview

Online Datasheet in PDF

Product Name

Probable E3 ubiquitin-protein ligase makorin-2 (MKRN2), ELISA Kit

Full Product Name

Human Probable E3 ubiquitin-protein ligase makorin-2 (MKRN2) ELISA Kit

Product Gene Name

MKRN2 elisa kit

Species Reactivity

Human

Assay Type

Sandwich

Samples

Serum, Plasma, Other biological fluids

Specificity

This assay has high sensitivity and excellent specificity for detection of Human MKRN2. No significant cross-reactivity or interference between Human MKRN2 and analogues was observed.

Preparation and Storage

Store at 2-8 degree C.

Molecular Weight

41,985 Da

OMIM

AF302084 mRNA

3D Structure

ModBase 3D Structure for Q9H000

Sample Volume

1-200 uL

Precision

Intra-assay Precision (Precision within an assay)
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%) = SD/meanX100
Intra-Assay: CV
Inter-Assay: CV

Detection Wavelength

450 nm

ISO Certification

Manufactured in an ISO 9001:2015 Certified Laboratory.

Other Notes

Small volumes of MKRN2 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for MKRN2 purchase

MBS283514 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Probable E3 ubiquitin-protein ligase makorin-2 (MKRN2) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing MKRN2. The ELISA analytical biochemical technique of the MBS283514 kit is based on MKRN2 antibody-MKRN2 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect MKRN2 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, MKRN2. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for MKRN2. It may not necessarily be applicable to this product.

NCBI GI #

411147393

NCBI GeneID

23609

NCBI Accession #

NP_001258636.1

UniProt Primary Accession #

Q9H000

UniProt Secondary Accession #

Q8N391; Q96BD4; Q9BUY2; Q9NRY1; A6NIA2; B3KRC5; B4DPR4

UniProt Related Accession #

Q9H000

GenBank Nucleotide #

NM_001271707.1

NCBI Official Full Name

probable E3 ubiquitin-protein ligase makorin-2 isoform 2

NCBI Official Synonym Full Names

makorin ring finger protein 2

NCBI Official Symbol

MKRN2

NCBI Official Synonym Symbols

RNF62; HSPC070

UniProt Protein Name

Probable E3 ubiquitin-protein ligase makorin-2

UniProt Synonym Protein Names

RING finger protein 62; RING-type E3 ubiquitin transferase makorin-2Curated

Protein Family

Probable E3 ubiquitin-protein ligase makorin

UniProt Gene Name

MKRN2

UniProt Synonym Gene Names

RNF62

NCBI Protein Information

probable E3 ubiquitin-protein ligase makorin-2

NCBI Summary for MKRN2

This gene encodes a probable E3 ubiquitin ligase containing several zinc finger domains, that is a member of the makorin RING zinc-finger protein family. This gene overlaps the v-raf-1 murine leukemia viral oncogene homolog 1 (RAF1) gene in an antisense orientation and may have a co-regulatory function with RAF1. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2012]

UniProt Comments for MKRN2

MKRN2: E3 ubiquitin ligase catalyzing the covalent attachment of ubiquitin moieties onto substrate proteins.

Protein type: EC 6.3.2.-; EC 6.3.2.19; Ubiquitin conjugating system; Ubiquitin ligase

Chromosomal Location of Human Ortholog: 3p25.2

Cellular Component: intracellular

Molecular Function: metal ion binding; protein binding; RNA binding; transferase activity

Biological Process: protein ubiquitination

Research Articles on MKRN2

1. Over-expression of MKRN2 in K562 cells increased cell proliferation. Our results indicated possible roles of MKRN2 in normal and malignant hematopoiesis.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Diseases associated with MKRN2 elisa kit

Disease Name	Pubmed Publications
Carcinoma Antibodies	>1 publications with MKRN2 and Carcinoma

Organs/Tissues associated with MKRN2 elisa kit

Organ/Tissue Name	Pubmed Publications
Nerve Antibodies	>2 publications with MKRN2 and Nerve
Thyroid Antibodies	>1 publications with MKRN2 and Thyroid
Blood Antibodies	>1 publications with MKRN2 and Blood
Skin Antibodies	>1 publications with MKRN2 and Skin
Umbilical Cord Antibodies	>1 publications with MKRN2 and Umbilical Cord
Testis Antibodies	>1 publications with MKRN2 and Testis
Brain Antibodies	>1 publications with MKRN2 and Brain