Human MARCH10 ELISA Kit – Cat# MBS283516

Probable E3 Ubiquitin-Protein Ligase MARCH10 (MARCH10) ELISA Kit for Human

Name: Human MARCH10 ELISA Kit; Probable E3 Ubiquitin-Protein Ligase MARCH10; MyBioSource
Brand: MyBioSource
SKU: MBS283516
Price: 495 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS283516

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate MARCH10 in samples. An antibody specific for MARCH10 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMARCH10 present is bound by… More details

$495 / 48 Tests +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

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Product Overview

Online Datasheet in PDF

Product Name

Probable E3 ubiquitin-protein ligase MARCH10, ELISA Kit

Full Product Name

Human Probable E3 ubiquitin-protein ligase MARCH10 (MARCH10) ELISA Kit

Product Gene Name

MARCH10 elisa kit

Species Reactivity

Human

Assay Type

Sandwich

Samples

Serum, Plasma, Other biological fluids

Specificity

This assay has high sensitivity and excellent specificity for detection of Human MARCH10. No significant cross-reactivity or interference between Human MARCH10 and analogues was observed.

Preparation and Storage

Store at 2-8 degree C.

Molecular Weight

90,511 Da

OMIM

AK093076 mRNA

3D Structure

ModBase 3D Structure for Q8NA82

Sample Volume

1-200 uL

Precision

Intra-assay Precision (Precision within an assay)
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%) = SD/meanX100
Intra-Assay: CV
Inter-Assay: CV

Detection Wavelength

450 nm

ISO Certification

Manufactured in an ISO 9001:2015 Certified Laboratory.

Other Notes

Small volumes of MARCH10 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for MARCH10 purchase

MBS283516 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Probable E3 ubiquitin-protein ligase MARCH10, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing MARCH10. The ELISA analytical biochemical technique of the MBS283516 kit is based on MARCH10 antibody-MARCH10 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect MARCH10 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, MARCH10. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for MARCH10. It may not necessarily be applicable to this product.

NCBI GI #

154937342

NCBI GeneID

162333

NCBI Accession #

NP_001094345.1

UniProt Primary Accession #

Q8NA82

UniProt Secondary Accession #

Q8IYS7; Q8N7Z7; D3DU09

UniProt Related Accession #

Q8NA82

GenBank Nucleotide #

NM_001100875.2

NCBI Official Full Name

probable E3 ubiquitin-protein ligase MARCH10 isoform 1

NCBI Official Synonym Full Names

membrane associated ring-CH-type finger 10

NCBI Official Symbol

MARCH10

NCBI Official Synonym Symbols

RNF190; MARCH-X

UniProt Protein Name

Probable E3 ubiquitin-protein ligase MARCH10

UniProt Synonym Protein Names

Membrane-associated RING finger protein 10; Membrane-associated RING-CH protein X; MARCH-X; RING finger protein 190; RING-type E3 ubiquitin transferase MARCH10Curated

Protein Family

Probable E3 ubiquitin-protein ligase

UniProt Gene Name

MARCH10

UniProt Synonym Gene Names

RNF190; MARCH-X

NCBI Protein Information

probable E3 ubiquitin-protein ligase MARCH10

NCBI Summary for MARCH10

MARCH10 is a member of the MARCH family of membrane-bound E3 ubiquitin ligases (EC 6.3.2.19). MARCH enzymes add ubiquitin (see MIM 191339) to target lysines in substrate proteins, thereby signaling their vesicular transport between membrane compartments (Morokuma et al., 2007 [PubMed 17604280]).[supplied by OMIM, Apr 2010]

UniProt Comments for MARCH10

MARCH10: E3 ubiquitin-protein ligase (Probable). E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfer the ubiquitin to targeted substrates.

Protein type: EC 6.3.2.-; EC 6.3.2.19; Ligase; Ubiquitin conjugating system; Ubiquitin ligase

Chromosomal Location of Human Ortholog: 17q23.2

Molecular Function: protein binding; transferase activity; zinc ion binding

Biological Process: protein ubiquitination

Research Articles on MARCH10

1. Observational study of gene-disease association. (HuGE Navigator)

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Organs/Tissues associated with MARCH10 elisa kit

Organ/Tissue Name	Pubmed Publications
Testis Antibodies	>1 publications with MARCH10 and Testis