Xaa-Pro Dipeptidase (PEPD) ELISA Kit for Mouse

Name: Mouse PEPD ELISA Kit; Xaa-Pro Dipeptidase; MyBioSource
Brand: MyBioSource
SKU: MBS283559
Price: 495 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS283559

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate PEPD in samples. An antibody specific for PEPD has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPEPD present is bound by the… More details

$495 / 48 Tests +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Request Current Manual

Product Overview

Online Datasheet in PDF

Product Name

Xaa-Pro dipeptidase (PEPD), ELISA Kit

Full Product Name

Mouse Xaa-Pro dipeptidase (PEPD) ELISA Kit

Product Gene Name

PEPD elisa kit

Species Reactivity

Mouse

Assay Type

Sandwich

Samples

Serum, Plasma, Other biological fluids

Specificity

This assay has high sensitivity and excellent specificity for detection of Mouse PEPD. No significant cross-reactivity or interference between Mouse PEPD and analogues was observed.

Preparation and Storage

Store at 2-8 degree C.

Molecular Weight

55,029 Da

3D Structure

ModBase 3D Structure for Q11136

Sample Volume

1-200 uL

Precision

Intra-assay Precision (Precision within an assay)
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%) = SD/meanX100
Intra-Assay: CV
Inter-Assay: CV

Detection Wavelength

450 nm

ISO Certification

Manufactured in an ISO 9001:2015 Certified Laboratory.

Other Notes

Small volumes of PEPD elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for PEPD purchase

MBS283559 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Xaa-Pro dipeptidase (PEPD) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing PEPD. The ELISA analytical biochemical technique of the MBS283559 kit is based on PEPD antibody-PEPD antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect PEPD antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, PEPD. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for PEPD. It may not necessarily be applicable to this product.

NCBI GI #

170650724

NCBI GeneID

18624

NCBI Accession #

NP_032846.2

UniProt Primary Accession #

Q11136

UniProt Secondary Accession #

P97735; Q3TH86; Q9CWK4

UniProt Related Accession #

Q11136

GenBank Nucleotide #

NM_008820.2

NCBI Official Full Name

xaa-Pro dipeptidase

NCBI Official Synonym Full Names

peptidase D

NCBI Official Symbol

Pepd

NCBI Official Synonym Symbols

dal; Pep4; Pep-4

UniProt Protein Name

Xaa-Pro dipeptidase

UniProt Synonym Protein Names

Imidodipeptidase; Peptidase 4; Peptidase D; Proline dipeptidase; Prolidase

Protein Family

Cytosol non-specific dipeptidase

UniProt Gene Name

Pepd

UniProt Synonym Gene Names

Pep4; X-Pro dipeptidase; Prolidase

NCBI Protein Information

xaa-Pro dipeptidase

UniProt Comments for PEPD

peptidase D: Splits dipeptides with a prolyl or hydroxyprolyl residue in the C-terminal position. Plays an important role in collagen metabolism because the high level of iminoacids in collagen. Defects in PEPD are a cause of prolidase deficiency (PD). Prolidase deficiency is an autosomal recessive disorder associated with iminodipeptiduria. The clinical phenotype includes skin ulcers, mental retardation, recurrent infections, and a characteristic facies. These features, however are incompletely penetrant and highly variable in both age of onset and severity. There is a tight linkage between the polymorphisms of prolidase and the myotonic dystrophy trait. Belongs to the peptidase M24B family. Eukaryotic-type prolidase subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.4.13.9; Protease

Chromosomal Location of Human Ortholog: 7 B2|7 20.7 cM

Cellular Component: extracellular exosome

Molecular Function: aminopeptidase activity; dipeptidase activity; hydrolase activity; manganese ion binding; metal ion binding; metallopeptidase activity; peptidase activity

Biological Process: collagen catabolic process; proteolysis

Research Articles on PEPD

1. The findings identify peptidase D, an ubiquitously expressed intracellular peptidase, as a potential novel marker of colitis.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Diseases associated with PEPD elisa kit

Disease Name	Pubmed Publications
Prolidase Deficiency Antibodies	>19 publications with PEPD and Prolidase Deficiency
Vascular Diseases Antibodies	>8 publications with PEPD and Vascular Diseases
Infection Antibodies	>6 publications with PEPD and Infection
Splenomegaly Antibodies	>4 publications with PEPD and Splenomegaly
Hypertrophy Antibodies	>3 publications with PEPD and Hypertrophy
Skin Ulcer Antibodies	>3 publications with PEPD and Skin Ulcer
Respiratory Tract Diseases Antibodies	>3 publications with PEPD and Respiratory Tract Diseases
Diabetes Mellitus, Type 2 Antibodies	>3 publications with PEPD and Diabetes Mellitus, Type 2
Liver Diseases Antibodies	>2 publications with PEPD and Liver Diseases
Kidney Diseases Antibodies	>1 publications with PEPD and Kidney Diseases

Organs/Tissues associated with PEPD elisa kit

Organ/Tissue Name	Pubmed Publications
Skin Antibodies	>14 publications with PEPD and Skin
Blood Antibodies	>9 publications with PEPD and Blood
Muscle Antibodies	>5 publications with PEPD and Muscle
Kidney Antibodies	>3 publications with PEPD and Kidney
Ovary Antibodies	>1 publications with PEPD and Ovary
Heart Antibodies	>1 publications with PEPD and Heart
Lung Antibodies	>1 publications with PEPD and Lung
Pancreas Antibodies	>1 publications with PEPD and Pancreas
Embryonic Tissue Antibodies	>1 publications with PEPD and Embryonic Tissue