Potassium/Sodium Hyperpolarization-Activated Cyclic Nucleotide-Gated Channel 2 (HCN2) ELISA Kit for Human

Name: Human HCN2 ELISA Kit; MyBioSource
Brand: MyBioSource
SKU: MBS283560
Price: 495 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS283560

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate HCN2 in samples. An antibody specific for HCN2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyHCN2 present is bound by the… More details

$495 / 48 Tests +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

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Product Overview

Online Datasheet in PDF

Product Name

Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 2 (HCN2), ELISA Kit

Full Product Name

Human Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 2 (HCN2) ELISA Kit

Product Gene Name

HCN2 elisa kit

Species Reactivity

Human

Assay Type

Sandwich

Samples

Serum, Plasma, Other biological fluids

Specificity

This assay has high sensitivity and excellent specificity for detection of Human HCN2. No significant cross-reactivity or interference between Human HCN2 and analogues was observed.

Preparation and Storage

Store at 2-8 degree C.

Molecular Weight

96,950 Da

OMIM

AF065164 mRNA

3D Structure

ModBase 3D Structure for Q9UL51

Sample Volume

1-200 uL

Precision

Intra-assay Precision (Precision within an assay)
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%) = SD/meanX100
Intra-Assay: CV
Inter-Assay: CV

Detection Wavelength

450 nm

ISO Certification

Manufactured in an ISO 9001:2015 Certified Laboratory.

Other Notes

Small volumes of HCN2 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for HCN2 purchase

MBS283560 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 2 (HCN2) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing HCN2. The ELISA analytical biochemical technique of the MBS283560 kit is based on HCN2 antibody-HCN2 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect HCN2 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, HCN2. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for HCN2. It may not necessarily be applicable to this product.

NCBI GI #

156071470

NCBI GeneID

610

NCBI Accession #

NP_001185.3

UniProt Primary Accession #

Q9UL51

UniProt Secondary Accession #

O60742; O60743; O75267; Q9UBS2

UniProt Related Accession #

Q9UL51

GenBank Nucleotide #

NM_001194.3

NCBI Official Full Name

potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 2

NCBI Official Synonym Full Names

hyperpolarization activated cyclic nucleotide gated potassium and sodium channel 2

NCBI Official Symbol

HCN2

NCBI Official Synonym Symbols

BCNG2; HAC-1; BCNG-2

UniProt Protein Name

Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 2

UniProt Synonym Protein Names

Brain cyclic nucleotide-gated channel 2; BCNG-2

Protein Family

Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel

UniProt Gene Name

HCN2

UniProt Synonym Gene Names

BCNG2; BCNG-2

NCBI Protein Information

potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 2

NCBI Summary for HCN2

The protein encoded by this gene is a hyperpolarization-activated cation channel involved in the generation of native pacemaker activity in the heart and in the brain. The encoded protein is activated by cAMP and can produce a fast, large current. Defects in this gene were noted as a possible cause of some forms of epilepsy. [provided by RefSeq, Jan 2017]

UniProt Comments for HCN2

HCN2: Hyperpolarization-activated ion channel exhibiting weak selectivity for potassium over sodium ions. Contributes to the native pacemaker currents in heart (If) and in neurons (Ih). Produces a large instantaneous current. Activated by cAMP. Modulated by intracellular chloride ions and pH; acidic pH shifts the activation to more negative voltages. Belongs to the potassium channel HCN family.

Protein type: Channel, cation; Membrane protein, integral; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 19p13.3

Cellular Component: HCN channel complex; integral component of plasma membrane; plasma membrane; voltage-gated potassium channel complex

Molecular Function: cAMP binding; identical protein binding; intracellular cAMP activated cation channel activity; protein binding; voltage-gated potassium channel activity; voltage-gated sodium channel activity

Biological Process: cell-cell signaling; cellular response to cAMP; cellular response to cGMP; membrane depolarization during cardiac muscle cell action potential; potassium ion import across plasma membrane; potassium ion transmembrane transport; regulation of ion transmembrane transport; regulation of membrane potential; sodium ion import across plasma membrane

Research Articles on HCN2

1. beta2-adrenoceptors alone strongly enhanced the activity of HCN2 channels, and co-expression of odorant receptors further diversified the HCN2 channel activity.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with HCN2 elisa kit

Reference Product	PubMed Publications
HCN1 elisa kit	>191 publications with HCN2 and HCN1
HCN4 elisa kit	>160 publications with HCN2 and HCN4

Pathways associated with HCN2 elisa kit

Products by Pathway	Pathway Diagram
HCN Channels Pathway antibodies	HCN Channels Pathway Diagram
Neuronal System Pathway antibodies	Neuronal System Pathway Diagram
Potassium Channels Pathway antibodies	Potassium Channels Pathway Diagram
CAMP Signaling Pathway antibodies	CAMP Signaling Pathway Diagram
CAMP Signaling Pathway antibodies	CAMP Signaling Pathway Diagram

Diseases associated with HCN2 elisa kit

Disease Name	Pubmed Publications
Heart Diseases Antibodies	>47 publications with HCN2 and Heart Diseases
Cardiovascular Diseases Antibodies	>44 publications with HCN2 and Cardiovascular Diseases
Brain Diseases Antibodies	>36 publications with HCN2 and Brain Diseases
Arrhythmias, Cardiac Antibodies	>35 publications with HCN2 and Arrhythmias, Cardiac
Hypertrophy Antibodies	>11 publications with HCN2 and Hypertrophy
Bradycardia Antibodies	>9 publications with HCN2 and Bradycardia
Inflammation Antibodies	>9 publications with HCN2 and Inflammation
Congenital Abnormalities Antibodies	>5 publications with HCN2 and Congenital Abnormalities
Myocardial Infarction Antibodies	>4 publications with HCN2 and Myocardial Infarction
Learning Disorders Antibodies	>2 publications with HCN2 and Learning Disorders

Organs/Tissues associated with HCN2 elisa kit

Organ/Tissue Name	Pubmed Publications
Brain Antibodies	>108 publications with HCN2 and Brain
Embryonic Tissue Antibodies	>12 publications with HCN2 and Embryonic Tissue
Eye Antibodies	>6 publications with HCN2 and Eye
Pancreas Antibodies	>2 publications with HCN2 and Pancreas
Skin Antibodies	>1 publications with HCN2 and Skin