Human PGI Recombinant Protein – Cat# MBS283684

Prostacyclin (PGI) Human Recombinant Protein

Name: PGI Recombinant Protein; Prostacyclin; Human; MyBioSource
Brand: MyBioSource
SKU: MBS283684
Price: 530 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS283684

Prostacyclin is produced in endothelial cells from prostaglandin H2 (PGH2) by the action of the enzyme prostacyclin synthase. Although prostacyclin is considered an independent mediator, it is called PGI2 in eicosanoid nomenclature, and is a member of the… More details

$530 / 0.1 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

Prostacyclin (PGI), Recombinant Protein

Full Product Name

Recombinant Human Prostacyclin (PGI)

Product Gene Name

PGI recombinant protein

Product Synonym Names

Prostaglandin I2; PGI2.

Sequence

AIFLMERIFD VQLPHYSPSD EKARMKLTLL HRELQALTEA MYTNLHAVLL GDATEAGSGW HEMGLLDFS YSFLLRAGYL TLYGIEALPR THESQAQDRV HSADVFHTFR QLDRLLPKLA RGSLSVGDKD HMCSVKSRL WKLLSPARLA RRAHRSKWLE SYLLHLEEMG VSEEMQARAL VLQLWATQ

Host

E.coli AA 100-285.

Form/Format

Lyophilized Powder; The purified protein was Lyophilized from sterile PBS (58 mM Na2HPO4,17 mM NaH2PO4, 68 mM NaCl, pH 7.4). 5% trehalose and 5% mannitol are added as protectant before lyophilization. The elution buffer contain 300 mM imidazole.

Purity/Purification

>90 % as determined by SDS-PAGE

Preparation and Storage

Store it under sterile conditions at -20°C upon receiving. Recommend to aliquot the protein into smaller quantities for optimal storage. **Avoid repeated freeze-thaw cycles.**
Stability: The recombinant protein is stable for up to 6-12 months from date of receipt at -80°C.

Molecular Weight

Predicted MW: 24 kDa
Observed MW: 24 kDa

OMIM

600022

Source

Human

Protein Residue

N-terminal 6*His-tagged.

Usage

PGI Protein - Reconstitute at 0.25 ug/uL in 200 uL sterile water for short-term storage. Reconstitution with 200 uL 50% glycerol solution is recommended for longer term storage. If a different concentration is needed for your purposes please adjust the reconstitution volume as required (please note: the ion concentration of the final solution will vary according to the volume used).
Note: Centrifuge vial before opening. When reconstituting, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution.

ISO Certification

Manufactured in an ISO 9001:2015 Certified Laboratory.

Applications Tested/Suitable for PGI recombinant protein

Western Blot (WB), ELISA (EIA)

Application Notes for PGI recombinant protein

Western blotting, ELISA, Albumin-bound fluorescence was used in serum of patients with chronic renal failure.

Other Notes

Small volumes of PGI recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for PGI purchase

MBS283684 is a recombinant protein targeting PGI. Also known as Recombinant Human Prostacyclin (PGI). Host: E.coli AA 100-285.

Additional Details

NCBI/Uniprot data below describe general gene information for PGI. It may not necessarily be applicable to this product.

NCBI GI #

4506263

NCBI GeneID

5739

NCBI Accession #

NP_000951.1

UniProt Related Accession #

P43119

GenBank Nucleotide #

NM_000960.3

NCBI Official Full Name

prostacyclin receptor

NCBI Official Synonym Full Names

prostaglandin I2 (prostacyclin) receptor (IP)

NCBI Official Symbol

PTGIR

NCBI Official Synonym Symbols

IP; PRIPR

UniProt Protein Name

Prostacyclin receptor

UniProt Synonym Protein Names

Prostaglandin I2 receptor; PGI receptor; PGI2 receptor; Prostanoid IP receptor

Protein Family

Glucose-6-phosphate isomerase

UniProt Gene Name

PTGIR

UniProt Synonym Gene Names

PRIPR; PGI receptor; PGI2 receptor

UniProt Entry Name

PI2R_HUMAN

NCBI Protein Information

prostacyclin receptor; PGI receptor; PGI2 receptor; prostanoid IP receptor; prostaglandin I2 receptor

NCBI Summary for PGI

The protein encoded by this gene is a member of the G-protein coupled receptor family 1 and has been shown to be a receptor for prostacyclin. Prostacyclin, the major product of cyclooxygenase in macrovascular endothelium, elicits a potent vasodilation and inhibition of platelet aggregation through binding to this receptor. [provided by RefSeq, Jul 2008]

UniProt Comments for PGI

PTGIR: Receptor for prostacyclin (prostaglandin I2 or PGI2). The activity of this receptor is mediated by G(s) proteins which activate adenylate cyclase. Belongs to the G-protein coupled receptor 1 family.

Protein type: Receptor, GPCR; GPCR, family 1; Membrane protein, integral; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 19q13.3

Cellular Component: integral to plasma membrane; plasma membrane; cytosol

Molecular Function: G-protein coupled receptor activity; guanyl-nucleotide exchange factor activity

Biological Process: G-protein signaling, coupled to cyclic nucleotide second messenger; cell-cell signaling; negative regulation of smooth muscle cell proliferation; positive regulation of cAMP biosynthetic process; response to lipopolysaccharide; blood coagulation; G-protein signaling, adenylate cyclase activating pathway; positive regulation of GTPase activity

Research Articles on PGI

1. Prostaglandin I2- Prostaglandin I2 receptor signaling regulates human Th17 and Treg cell differentiation.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with PGI recombinant protein

Reference Product	PubMed Publications
ADRB2 recombinant protein	>3 publications with PGI and ADRB2

Pathways associated with PGI recombinant protein

Products by Pathway	Pathway Diagram
Class A/1 (Rhodopsin-like Receptors) Pathway antibodies	Class A/1 (Rhodopsin-like Receptors) Pathway Diagram
Eicosanoid Ligand-binding Receptors Pathway antibodies	Eicosanoid Ligand-binding Receptors Pathway Diagram
Endothelin Pathway antibodies	Endothelin Pathway Diagram
G Alpha (s) Signalling Events Pathway antibodies	G Alpha (s) Signalling Events Pathway Diagram
GPCR Downstream Signaling Pathway antibodies	GPCR Downstream Signaling Pathway Diagram
GPCR Ligand Binding Pathway antibodies	GPCR Ligand Binding Pathway Diagram
GPCRs, Class A Rhodopsin-like Pathway antibodies	GPCRs, Class A Rhodopsin-like Pathway Diagram
Hemostasis Pathway antibodies	Hemostasis Pathway Diagram
Neuroactive Ligand-receptor Interaction Pathway antibodies	Neuroactive Ligand-receptor Interaction Pathway Diagram
Neuroactive Ligand-receptor Interaction Pathway antibodies	Neuroactive Ligand-receptor Interaction Pathway Diagram

Diseases associated with PGI recombinant protein

Disease Name	Pubmed Publications
Cardiovascular Diseases Antibodies	>17 publications with PGI and Cardiovascular Diseases
Vascular Diseases Antibodies	>14 publications with PGI and Vascular Diseases
Inflammation Antibodies	>13 publications with PGI and Inflammation
Hypertension Antibodies	>11 publications with PGI and Hypertension
Disease Models, Animal Antibodies	>7 publications with PGI and Disease Models, Animal
Atherosclerosis Antibodies	>6 publications with PGI and Atherosclerosis
Pain Antibodies	>5 publications with PGI and Pain
Lung Diseases Antibodies	>4 publications with PGI and Lung Diseases
Heart Diseases Antibodies	>4 publications with PGI and Heart Diseases
Necrosis Antibodies	>3 publications with PGI and Necrosis

Organs/Tissues associated with PGI recombinant protein

Organ/Tissue Name	Pubmed Publications
Vascular Antibodies	>35 publications with PGI and Vascular
Blood Antibodies	>23 publications with PGI and Blood
Kidney Antibodies	>8 publications with PGI and Kidney
Lung Antibodies	>5 publications with PGI and Lung
Skin Antibodies	>2 publications with PGI and Skin
Embryonic Tissue Antibodies	>2 publications with PGI and Embryonic Tissue
Brain Antibodies	>1 publications with PGI and Brain
Connective Tissue Antibodies	>1 publications with PGI and Connective Tissue
Spleen Antibodies	>1 publications with PGI and Spleen
Eye Antibodies	>1 publications with PGI and Eye