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Myogenin (MYOG) ELISA Kit for Mouse

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS283808

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate MYOG in samples. An antibody specific for MYOG has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMYOG present is bound by the… More details

$495 / 48 Tests +1 FREE 8GB USB

Product Documentation

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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Myogenin, ELISA Kit
Full Product Name
Mouse Myogenin (MYOG) ELISA Kit
Product Gene Name
MYOG elisa kit
Product Synonym Names
MYF4, MYOGENIN, bHLHc3, Myogenic factor-4; myogenin|myogenic factor 4|myogenin
Species Reactivity
Mouse
Assay Type
Sandwich
Samples
Serum, Plasma, Other biological fluids
Specificity
This assay has high sensitivity and excellent specificity for detection of Mouse MYOG. No significant cross-reactivity or interference between Mouse MYOG and analogues was observed.
Preparation and Storage
Store at 2-8 degree C.
Molecular Weight
25,203 Da
3D Structure
ModBase 3D Structure for P12979
Sample Volume
1-200 uL
Precision
Intra-assay Precision (Precision within an assay)
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%) = SD/meanX100
Intra-Assay: CV
Inter-Assay: CV
Detection Wavelength
450 nm
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.

Other Notes

Small volumes of MYOG elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for MYOG purchase

MBS283808 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Myogenin, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing MYOG. The ELISA analytical biochemical technique of the MBS283808 kit is based on MYOG antibody-MYOG antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect MYOG antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, MYOG. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for MYOG. It may not necessarily be applicable to this product.

NCBI GI #
13654247
NCBI GeneID
17928
NCBI Accession #
NP_112466.1
UniProt Primary Accession #
P12979
UniProt Related Accession #
P12979
GenBank Nucleotide #
NM_031189.2
NCBI Official Full Name
myogenin
NCBI Official Synonym Full Names
myogenin
NCBI Official Symbol
Myog
NCBI Official Synonym Symbols
myo; MYF4; bHLHc3
UniProt Protein Name
Myogenin
UniProt Synonym Protein Names
MYOD1-related protein
Protein Family
Myogenin
UniProt Gene Name
Myog
NCBI Protein Information
myogenin

UniProt Comments for MYOG

myogenin: Involved in muscle differentiation (myogenic factor). Induces fibroblasts to differentiate into myoblasts. Probable sequence specific DNA-binding protein.

Protein type: DNA-binding; Transcription factor

Chromosomal Location of Human Ortholog: 1 E4|1 58.18 cM

Cellular Component: nucleoplasm; nucleus

Molecular Function: chromatin DNA binding; DNA binding; DNA binding transcription factor activity; protein binding; protein dimerization activity; sequence-specific DNA binding; transcription factor activity, RNA polymerase II distal enhancer sequence-specific binding

Biological Process: cell cycle; cell differentiation; cellular response to estradiol stimulus; cellular response to growth factor stimulus; cellular response to lithium ion; cellular response to tumor necrosis factor; mRNA transcription from RNA polymerase II promoter; multicellular organism development; muscle organ development; myotube differentiation; negative regulation of cell proliferation; negative regulation of glycolytic process; ossification; positive regulation of cell cycle arrest; positive regulation of muscle atrophy; positive regulation of myoblast differentiation; positive regulation of myotube differentiation; positive regulation of oxidative phosphorylation; positive regulation of skeletal muscle fiber development; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-templated; regulation of skeletal muscle satellite cell proliferation; regulation of transcription, DNA-templated; response to denervation involved in regulation of muscle adaptation; response to electrical stimulus involved in regulation of muscle adaptation; response to muscle activity involved in regulation of muscle adaptation; skeletal muscle fiber development; skeletal muscle tissue development; striated muscle atrophy; transcription, DNA-dependent
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Research Articles on MYOG

1. Data show that MyoD and myogenin associate with distinct chromatin states.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with MYOG elisa kit

 Reference Product  PubMed Publications
 Myf5 elisa kit  >198 publications with MYOG and Myf5
 Myod1 elisa kit  >189 publications with MYOG and Myod1
 Myf6 elisa kit  >32 publications with MYOG and Myf6
 Mef2c elisa kit  >21 publications with MYOG and Mef2c
 Mef2a elisa kit  >17 publications with MYOG and Mef2a
 Mef2d elisa kit  >4 publications with MYOG and Mef2d
 Mapk14 elisa kit  >2 publications with MYOG and Mapk14
 Tcf3 elisa kit  >2 publications with MYOG and Tcf3

Pathways associated with MYOG elisa kit

 Products by Pathway  Pathway Diagram
 CDO In Myogenesis Pathway antibodies  CDO In Myogenesis Pathway Diagram
 Developmental Biology Pathway antibodies  Developmental Biology Pathway Diagram
 Hypertrophy Model Pathway antibodies  Hypertrophy Model Pathway Diagram
 Id Signaling Pathway antibodies  Id Signaling Pathway Diagram
 Myogenesis Pathway antibodies  Myogenesis Pathway Diagram

Diseases associated with MYOG elisa kit

 Disease Name  Pubmed Publications
 Atrophy Antibodies  >41 publications with MYOG and Atrophy
 Hypertrophy Antibodies  >35 publications with MYOG and Hypertrophy
 Necrosis Antibodies  >22 publications with MYOG and Necrosis
 Inflammation Antibodies  >17 publications with MYOG and Inflammation
 Fibrosis Antibodies  >17 publications with MYOG and Fibrosis
 Insulin Resistance Antibodies  >12 publications with MYOG and Insulin Resistance
 Hyperplasia Antibodies  >11 publications with MYOG and Hyperplasia
 Embryo Loss Antibodies  >9 publications with MYOG and Embryo Loss
 Cell Transformation, Neoplastic Antibodies  >9 publications with MYOG and Cell Transformation, Neoplastic
 Weight Loss Antibodies  >8 publications with MYOG and Weight Loss

Organs/Tissues associated with MYOG elisa kit

 Organ/Tissue Name  Pubmed Publications
 Muscle Antibodies  >875 publications with MYOG and Muscle
 Embryonic Tissue Antibodies  >151 publications with MYOG and Embryonic Tissue
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