DNA Damage-Inducible Transcript 4 (DDIT4) ELISA Kit for Rat

The 232-amino acid human protein has a calculated molecular mass of 25 kD and shares 90% amino acid conservation with the rat protein. In an in vitro translation assay, both rat and human RTP801 cDNAs gave rise to 35-kD proteins. Northern blot analysis of human tissues detected low-level ubiquitous expression of a 1.8-kb transcript. Lowest expression was in brain, skeletal muscle, and intestine.The predicted human REDD1 protein is acidic and rich in serine. It has orthologs in mouse, Xenopus, and Drosophila, with strongest evolutionarily sequence conservation at the C terminus. A related human transcript encodes a protein with 50% overall identity to REDD1, which the authors termed REDD2.

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate DDIT4 in samples. An antibody specific for DDIT4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyDDIT4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DDIT4 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DDIT4 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Small volumes of DDIT4 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS283811 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the DNA damage-inducible transcript 4 protein (DDIT4) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing DDIT4. The ELISA analytical biochemical technique of the MBS283811 kit is based on DDIT4 antibody-DDIT4 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect DDIT4 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, DDIT4. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).