Interleukin-10 (IL10RA) ELISA Kit for Human

Name: Human IL10RA ELISA Kit; Interleukin-10; MyBioSource
Brand: MyBioSource
SKU: MBS283827
Price: 495 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS283827

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate IL10RA in samples. An antibody specific for IL10RA has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyIL10RA present is bound by… More details

$495 / 48 Tests +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

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Product Overview

Online Datasheet in PDF

Product Name

Interleukin-10 (IL10RA), ELISA Kit

Full Product Name

Human Interleukin-10 receptor subunit alpha (IL10RA) ELISA Kit

Product Gene Name

IL10RA elisa kit

Product Synonym Names

CDW210A; HIL-10R; IL-10R1; IL10R; interleukin-10 receptor alpha chain

Species Reactivity

Human

Assay Type

Sandwich

Samples

Serum, Plasma, Other biological fluids

Specificity

This assay has high sensitivity and excellent specificity for detection of Human IL10RA. No significant cross-reactivity or interference between Human IL10RA and analogues was observed.

Preparation and Storage

Store at 2-8 degree C.

Molecular Weight

63,003 Da

OMIM

U00672 mRNA

3D Structure

ModBase 3D Structure for Q13651

Sample Volume

1-200 uL

Precision

Intra-assay Precision (Precision within an assay)
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%) = SD/meanX100
Intra-Assay: CV
Inter-Assay: CV

Detection Wavelength

450 nm

ISO Certification

Manufactured in an ISO 9001:2015 Certified Laboratory.

Other Notes

Small volumes of IL10RA elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for IL10RA purchase

MBS283827 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Interleukin-10 (IL10RA) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing IL10RA. The ELISA analytical biochemical technique of the MBS283827 kit is based on IL10RA antibody-IL10RA antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect IL10RA antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, IL10RA. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for IL10RA. It may not necessarily be applicable to this product.

NCBI GI #

222136575

NCBI GeneID

3587

NCBI Accession #

NP_001549.2

UniProt Primary Accession #

Q13651

UniProt Secondary Accession #

A8K6I0; B0YJ27

UniProt Related Accession #

Q13651

GenBank Nucleotide #

NM_001558.3

NCBI Official Full Name

interleukin-10 receptor subunit alpha

NCBI Official Synonym Full Names

interleukin 10 receptor subunit alpha

NCBI Official Symbol

IL10RA

NCBI Official Synonym Symbols

CD210; IL10R; CD210a; CDW210A; HIL-10R; IL-10R1

UniProt Protein Name

Interleukin-10 receptor subunit alpha

UniProt Synonym Protein Names

CDw210a; Interleukin-10 receptor subunit 1; IL-10R subunit 1; IL-10R1; CD_antigen: CD210

Protein Family

Interleukin-10 receptor

UniProt Gene Name

IL10RA

UniProt Synonym Gene Names

IL10R; IL-10 receptor subunit alpha; IL-10R subunit alpha; IL-10RA; IL-10R subunit 1; IL-10R1

NCBI Protein Information

interleukin-10 receptor subunit alpha

NCBI Summary for IL10RA

The protein encoded by this gene is a receptor for interleukin 10. This protein is structurally related to interferon receptors. It has been shown to mediate the immunosuppressive signal of interleukin 10, and thus inhibits the synthesis of proinflammatory cytokines. This receptor is reported to promote survival of progenitor myeloid cells through the insulin receptor substrate-2/PI 3-kinase/AKT pathway. Activation of this receptor leads to tyrosine phosphorylation of JAK1 and TYK2 kinases. Two transcript variants, one protein-coding and the other not protein-coding, have been found for this gene. [provided by RefSeq, Jan 2009]

UniProt Comments for IL10RA

IL10RA: a receptor for interleukin 10 structurally related to interferon receptors. Mediates the immunosuppressive signal of interleukin 10, and thus inhibits the synthesis of proinflammatory cytokines. This receptor is reported to promote survival of progenitor myeloid cells through the insulin receptor substrate-2/PI 3-kinase/AKT pathway. Activation of this receptor leads to tyrosine phosphorylation of JAK1 and TYK2 kinases.

Protein type: Membrane protein, integral; Receptor, cytokine

Chromosomal Location of Human Ortholog: 11q23.3

Cellular Component: integral component of membrane; plasma membrane

Molecular Function: interleukin-10 binding; interleukin-10 receptor activity; protein binding; receptor activity; signal transducer activity

Biological Process: cytokine-mediated signaling pathway; regulation of synapse organization; response to lipopolysaccharide

Disease: Inflammatory Bowel Disease 28, Autosomal Recessive

Research Articles on IL10RA

1. these findings provide a novel mechanism by which microbial-derived butyrate promotes barrier through IL-10RA-dependent repression of claudin-2

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with IL10RA elisa kit

Reference Product	PubMed Publications
IL10 elisa kit	>19 publications with IL10RA and IL10
IL10RB elisa kit	>10 publications with IL10RA and IL10RB
JAK1 elisa kit	>1 publications with IL10RA and JAK1

Pathways associated with IL10RA elisa kit

Products by Pathway	Pathway Diagram
Cytokine-cytokine Receptor Interaction Pathway antibodies	Cytokine-cytokine Receptor Interaction Pathway Diagram
Cytokine-cytokine Receptor Interaction Pathway antibodies	Cytokine-cytokine Receptor Interaction Pathway Diagram
Epstein-Barr Virus Infection Pathway antibodies	Epstein-Barr Virus Infection Pathway Diagram
Epstein-Barr Virus Infection Pathway antibodies	Epstein-Barr Virus Infection Pathway Diagram
Jak-STAT Signaling Pathway antibodies	Jak-STAT Signaling Pathway Diagram
Jak-STAT Signaling Pathway antibodies	Jak-STAT Signaling Pathway Diagram
Toxoplasmosis Pathway antibodies	Toxoplasmosis Pathway Diagram
Toxoplasmosis Pathway antibodies	Toxoplasmosis Pathway Diagram
Tuberculosis Pathway antibodies	Tuberculosis Pathway Diagram
Tuberculosis Pathway antibodies	Tuberculosis Pathway Diagram

Diseases associated with IL10RA elisa kit

Disease Name	Pubmed Publications
Inflammation Antibodies	>10 publications with IL10RA and Inflammation
Cardiovascular Diseases Antibodies	>4 publications with IL10RA and Cardiovascular Diseases
Neoplasms Antibodies	>4 publications with IL10RA and Neoplasms
Necrosis Antibodies	>4 publications with IL10RA and Necrosis
Brain Diseases Antibodies	>2 publications with IL10RA and Brain Diseases
Liver Diseases Antibodies	>2 publications with IL10RA and Liver Diseases
Skin Diseases Antibodies	>2 publications with IL10RA and Skin Diseases
Disease Models, Animal Antibodies	>1 publications with IL10RA and Disease Models, Animal
Lung Diseases Antibodies	>1 publications with IL10RA and Lung Diseases
Kidney Diseases Antibodies	>1 publications with IL10RA and Kidney Diseases

Organs/Tissues associated with IL10RA elisa kit

Organ/Tissue Name	Pubmed Publications
Blood Antibodies	>15 publications with IL10RA and Blood
Brain Antibodies	>3 publications with IL10RA and Brain
Bone Antibodies	>3 publications with IL10RA and Bone
Skin Antibodies	>2 publications with IL10RA and Skin
Vascular Antibodies	>2 publications with IL10RA and Vascular
Tonsil Antibodies	>1 publications with IL10RA and Tonsil
Pharynx Antibodies	>1 publications with IL10RA and Pharynx
Lung Antibodies	>1 publications with IL10RA and Lung
Spleen Antibodies	>1 publications with IL10RA and Spleen
Thymus Antibodies	>1 publications with IL10RA and Thymus