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DNA (Cytosine-5)-Methyltransferase 1 (DNMT1) ELISA Kit for Rat

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS283865

DNA (cytosine-5)-methyltransferases (DNMTs), such as DNMT1, maintain patterns of methylated cytosine residues in the mammalian genome. Genomic methylation patterns are reshaped during gametogenesis and early development and undergo programmed alterations… More details

$495 / 48 Tests +1 FREE 8GB USB

Product Documentation

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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
DNA (cytosine-5)-methyltransferase 1 (DNMT1), ELISA Kit
Full Product Name
Rat DNA (cytosine-5)-methyltransferase 1 (DNMT1) ELISA Kit
Product Gene Name
DNMT1 elisa kit
Product Synonym Names
AIM; CXXC9; DNMT; FLJ16293; MCMT; MGC104992; CXXC finger protein 9|DNA methyltransferase 1
Species Reactivity
Rat
Assay Type
Sandwich
Samples
Serum, Plasma, Other biological fluids
Specificity
This assay has high sensitivity and excellent specificity for detection of Rat DNMT1. No significant cross-reactivity or interference between Rat DNMT1 and analogues was observed.
Preparation and Storage
Store at 2-8 degree C.
Molecular Weight
169,645 Da
3D Structure
ModBase 3D Structure for Q9Z330
Sample Volume
1-200 uL
Precision
Intra-assay Precision (Precision within an assay)
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%) = SD/meanX100
Intra-Assay: CV
Inter-Assay: CV
Detection Wavelength
450 nm
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.

Other Notes

Small volumes of DNMT1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for DNMT1 purchase

MBS283865 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the DNA (cytosine-5)-methyltransferase 1 (DNMT1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing DNMT1. The ELISA analytical biochemical technique of the MBS283865 kit is based on DNMT1 antibody-DNMT1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect DNMT1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, DNMT1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for DNMT1. It may not necessarily be applicable to this product.

NCBI GI #
20137608
NCBI GeneID
84350
NCBI Accession #
Q9Z330.2
UniProt Primary Accession #
Q9Z330
UniProt Secondary Accession #
P70487; Q9R252; Q9WTX3; Q9WU57
UniProt Related Accession #
Q9Z330
NCBI Official Full Name
DNA (cytosine-5)-methyltransferase 1
NCBI Official Synonym Full Names
DNA methyltransferase 1
NCBI Official Symbol
Dnmt1
UniProt Protein Name
DNA (cytosine-5)-methyltransferase 1
UniProt Synonym Protein Names
DNA MTase RnoIP; M.RnoIP; DNA methyltransferase I; MCMT
Protein Family
DNA
UniProt Gene Name
Dnmt1
UniProt Synonym Gene Names
Dnmt1; M.RnoIP
NCBI Protein Information
DNA (cytosine-5)-methyltransferase 1

NCBI Summary for DNMT1

methylates genomic DNA; involved in processes of gene inactivation, chromatin organization, X chromosome inactivation and genomic imprinting [RGD, Feb 2006]

UniProt Comments for DNMT1

DNMT1: an ubiquitous DNA methyltransferase that methylates CpG residues. Preferentially methylates hemimethylated DNA. It is responsible for maintaining methylation patterns established in development, and may play an active role in DNA damage repair. Mediates transcriptional repression by direct binding to HDAC2. Its abundance is reduced to non detectable levels at the G0 phase of the cell cycle and is dramatically induced upon entrance into the S-phase of the cell cycle. Interacts with HDAC1 and with PCNA. Forms a complex with DMAP1 and HDAC2, with direct interaction. Forms also a stable complex with E2F1, BB1 and HDAC1. Binds MBD2 and MBD3. Three isoforms of the human protein produced by alternative splicing have been described.

Protein type: Amino Acid Metabolism - cysteine and methionine; Cell development/differentiation; EC 2.1.1.37; Methyltransferase; Methyltransferase, DNA; Transcription regulation

Chromosomal Location of Human Ortholog: 8q13

Cellular Component: cell soma; centric heterochromatin; cytoplasm; heterochromatin; nucleus; protein complex; replication fork

Molecular Function: chromatin binding; DNA (cytosine-5-)-methyltransferase activity; DNA binding; DNA-methyltransferase activity; estrogen receptor binding; histone deacetylase binding; methyl-CpG binding; methyltransferase activity; protein binding; protein domain specific binding; RNA binding; S-adenosylmethionine-dependent methyltransferase activity; zinc ion binding

Biological Process: aging; brain development; covalent chromatin modification; cytosine methylation within a CG sequence; DNA methylation; DNA methylation involved in embryo development; DNA methylation on cytosine; gene silencing; maintenance of DNA methylation; negative regulation of histone H3-K9 methylation; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; neuron differentiation; positive regulation of histone H3-K4 methylation; Ras protein signal transduction; regulation of cell proliferation; regulation of gene expression; response to activity; response to caffeine; response to drug; response to estradiol; response to ethanol; response to heat; response to ionizing radiation; response to lead ion; response to lipopolysaccharide; response to nutrient levels; response to organic substance; response to testosterone stimulus; response to toxin; response to vitamin A; S-adenosylmethionine metabolic process; transcription, DNA-dependent
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Research Articles on DNMT1

1. hypomethylation of FABP4 was regulated by DNMT1, which was a direct target of miR-152/148a, in pre-eclampsia model

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with DNMT1 elisa kit

 Reference Product  PubMed Publications
 Dnmt3b elisa kit  >583 publications with DNMT1 and Dnmt3b
 Hdac1 elisa kit  >124 publications with DNMT1 and Hdac1
 Pcna elisa kit  >68 publications with DNMT1 and Pcna
 Ezh2 elisa kit  >45 publications with DNMT1 and Ezh2
 Hdac2 elisa kit  >45 publications with DNMT1 and Hdac2
 Tp53 elisa kit  >16 publications with DNMT1 and Tp53
 Usp7 elisa kit  >7 publications with DNMT1 and Usp7

Pathways associated with DNMT1 elisa kit

 Products by Pathway  Pathway Diagram
 Cysteine And Methionine Metabolism Pathway antibodies  Cysteine And Methionine Metabolism Pathway Diagram
 Cysteine And Methionine Metabolism Pathway antibodies  Cysteine And Methionine Metabolism Pathway Diagram
 Epigenetic Regulation Of Gene Expression Pathway antibodies  Epigenetic Regulation Of Gene Expression Pathway Diagram
 Gene Expression Pathway antibodies  Gene Expression Pathway Diagram
 Metabolic Pathways antibodies  Metabolic Pathways Diagram
 Methionine Degradation Pathway antibodies  Methionine Degradation Pathway Diagram
 Methionine Degradation Pathway antibodies  Methionine Degradation Pathway Diagram
 MicroRNAs In Cancer Pathway antibodies  MicroRNAs In Cancer Pathway Diagram
 MicroRNAs In Cancer Pathway antibodies  MicroRNAs In Cancer Pathway Diagram
 One Carbon Metabolism Pathway antibodies  One Carbon Metabolism Pathway Diagram

Organs/Tissues associated with DNMT1 elisa kit

 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >166 publications with DNMT1 and Brain
 Liver Antibodies  >139 publications with DNMT1 and Liver
 Lung Antibodies  >117 publications with DNMT1 and Lung
 Embryonic Tissue Antibodies  >54 publications with DNMT1 and Embryonic Tissue
 Kidney Antibodies  >41 publications with DNMT1 and Kidney
 Placenta Antibodies  >26 publications with DNMT1 and Placenta
 Eye Antibodies  >22 publications with DNMT1 and Eye
 Ovary Antibodies  >21 publications with DNMT1 and Ovary
 Pancreas Antibodies  >19 publications with DNMT1 and Pancreas
 Spleen Antibodies  >8 publications with DNMT1 and Spleen
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