Br Serine/Threonine-Protein Kinase 2 (BRSK2) ELISA Kit for Mouse

Name: Mouse BRSK2 ELISA Kit; Br Serine/Threonine-Protein Kinase 2; MyBioSource
Brand: MyBioSource
SKU: MBS283971
Price: 495 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS283971

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate BRSK2 in samples. An antibody specific for BRSK2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyBRSK2 present is bound by the… More details

$495 / 48 Tests +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

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Product Overview

Online Datasheet in PDF

Product Name

BR serine/threonine-protein kinase 2 (BRSK2), ELISA Kit

Full Product Name

Mouse BR serine/threonine-protein kinase 2 (BRSK2) ELISA Kit

Product Gene Name

BRSK2 elisa kit

Product Synonym Names

STK29; C11orf7; PEN11B; SADA; PEN11B; HUSSY-12; BR Serine/Threonine Kinase 2; Serine/Threonine Kinase 29; Brain-specific serine/threonine-protein kinase 2

Species Reactivity

Mouse

Assay Type

Sandwich

Samples

Serum, Plasma, Other biological fluids

Specificity

This assay has high sensitivity and excellent specificity for detection of Mouse BRSK2. No significant cross-reactivity or interference between Mouse BRSK2 and analogues was observed.

Preparation and Storage

Store at 2-8 degree C.

Molecular Weight

73,150 Da

3D Structure

ModBase 3D Structure for Q69Z98

Sample Volume

1-200 uL

Precision

Intra-assay Precision (Precision within an assay)
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%) = SD/meanX100
Intra-Assay: CV
Inter-Assay: CV

Detection Wavelength

450 nm

ISO Certification

Manufactured in an ISO 9001:2015 Certified Laboratory.

Other Notes

Small volumes of BRSK2 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for BRSK2 purchase

MBS283971 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the BR serine/threonine-protein kinase 2 (BRSK2) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing BRSK2. The ELISA analytical biochemical technique of the MBS283971 kit is based on BRSK2 antibody-BRSK2 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect BRSK2 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, BRSK2. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for BRSK2. It may not necessarily be applicable to this product.

NCBI GI #

58036483

NCBI GeneID

75770

NCBI Accession #

NP_001009929.1

UniProt Primary Accession #

Q69Z98

UniProt Secondary Accession #

Q699J3; Q699J4; Q6DMN7; Q6PHM0

UniProt Related Accession #

Q69Z98

GenBank Nucleotide #

NM_001009929.3

NCBI Official Full Name

serine/threonine-protein kinase BRSK2 isoform beta

NCBI Official Synonym Full Names

BR serine/threonine kinase 2

NCBI Official Symbol

Brsk2

NCBI Official Synonym Symbols

SADA; SAD-A; 4833424K13Rik

UniProt Protein Name

Serine/threonine-protein kinase BRSK2

UniProt Synonym Protein Names

Brain-specific serine/threonine-protein kinase 2; BR serine/threonine-protein kinase 2; Serine/threonine-protein kinase SAD-A

Protein Family

Serine/threonine-protein kinase

UniProt Gene Name

Brsk2

UniProt Synonym Gene Names

Kiaa4256; Sada; BR serine/threonine-protein kinase 2

NCBI Protein Information

serine/threonine-protein kinase BRSK2

UniProt Comments for BRSK2

BRSK2: a serine/threonine protein kinase of the CAMK group. Closely related to AMPK. Activated by the kinase LKB1. BRSK1 and BRSK2 are required for nueronal polarization. Expressed in the brain and at low levels in the testis. Four alternatively spliced isoforms have been described.

Protein type: BRSK subfamily; CAMK group; CAMKL family; EC 2.7.11.1; EC 2.7.11.26; Kinase, protein; Protein kinase, CAMK; Protein kinase, Ser/Thr (non-receptor)

Chromosomal Location of Human Ortholog: 7|7 F5

Cellular Component: centrosome; cytoplasm; cytoskeleton; endoplasmic reticulum; intracellular; nucleus

Molecular Function: ATP binding; ATPase binding; kinase activity; magnesium ion binding; metal ion binding; nucleotide binding; protein binding; protein kinase activity; protein kinase binding; protein serine/threonine kinase activity; tau-protein kinase activity; transferase activity

Biological Process: actin cytoskeleton reorganization; apoptosis; axonogenesis; cell cycle; cell division; establishment of cell polarity; exocytosis; G2/M transition of mitotic cell cycle; nervous system development; neuron differentiation; neuron projection morphogenesis; peptidyl-serine phosphorylation; phosphorylation; protein amino acid phosphorylation

Research Articles on BRSK2

1. Taken together, these data suggest a model for synergistic autoinhibition and membrane activation of SAD kinases.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Diseases associated with BRSK2 elisa kit

Disease Name	Pubmed Publications
Neoplasms Antibodies	>2 publications with BRSK2 and Neoplasms

Organs/Tissues associated with BRSK2 elisa kit

Organ/Tissue Name	Pubmed Publications
Brain Antibodies	>11 publications with BRSK2 and Brain
Testis Antibodies	>1 publications with BRSK2 and Testis
Spinal Cord Antibodies	>1 publications with BRSK2 and Spinal Cord