Erythroid Transcription Factor (GATA1) ELISA Kit for Rat

Name: Rat GATA1 ELISA Kit; Erythroid Transcription Factor; MyBioSource
Brand: MyBioSource
SKU: MBS283972
Price: 495 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS283972

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate GATA1 in samples. An antibody specific for GATA1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyGATA1 present is bound by the… More details

$495 / 48 Tests +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

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Product Overview

Online Datasheet in PDF

Product Name

Erythroid transcription factor (GATA1), ELISA Kit

Full Product Name

Rat Erythroid transcription factor (GATA1) ELISA Kit

Product Gene Name

GATA1 elisa kit

Product Synonym Names

ERYF1; GATA-1; GF-1; GF1; NFE1; XLTT; GATA binding protein 1|NF-E1 DNA-binding protein|erythroid transcription factor 1|globin transcription factor 1|transcription factor GATA1

Species Reactivity

Rat

Assay Type

Sandwich

Samples

Serum, Plasma, Other biological fluids

Specificity

This assay has high sensitivity and excellent specificity for detection of Rat GATA1. No significant cross-reactivity or interference between Rat GATA1 and analogues was observed.

Preparation and Storage

Store at 2-8 degree C.

Molecular Weight

42,871 Da

3D Structure

ModBase 3D Structure for P43429

Sample Volume

1-200 uL

Precision

Intra-assay Precision (Precision within an assay)
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%) = SD/meanX100
Intra-Assay: CV
Inter-Assay: CV

Detection Wavelength

450 nm

ISO Certification

Manufactured in an ISO 9001:2015 Certified Laboratory.

Other Notes

Small volumes of GATA1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for GATA1 purchase

MBS283972 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Erythroid transcription factor (GATA1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing GATA1. The ELISA analytical biochemical technique of the MBS283972 kit is based on GATA1 antibody-GATA1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect GATA1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, GATA1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for GATA1. It may not necessarily be applicable to this product.

NCBI GI #

6978877

NCBI GeneID

25172

NCBI Accession #

NP_036896.1

UniProt Primary Accession #

P43429

UniProt Related Accession #

P43429

GenBank Nucleotide #

NM_012764.1

NCBI Official Full Name

erythroid transcription factor

NCBI Official Synonym Full Names

GATA binding protein 1

NCBI Official Symbol

Gata1

NCBI Official Synonym Symbols

Eryf1

UniProt Protein Name

Erythroid transcription factor

UniProt Synonym Protein Names

Eryf1; GATA-binding factor 1; GATA-1; GF-1; NF-E1 DNA-binding protein

Protein Family

Erythroid transcription factor

UniProt Gene Name

Gata1

UniProt Synonym Gene Names

GATA-1; GF-1

NCBI Protein Information

erythroid transcription factor

NCBI Summary for GATA1

transcription factor that may regulate transcription of serine dehydratase [RGD, Feb 2006]

UniProt Comments for GATA1

GATA1: Transcriptional activator which probably serves as a general switch factor for erythroid development. It binds to DNA sites with the consensus sequence [AT]GATA[AG] within regulatory regions of globin genes and of other genes expressed in erythroid cells. May form homodimers or heterodimers with other isoforms. Interacts (via the N-terminal zinc finger) with ZFPM1. Interacts with GFI1B. Interacts with PIAS4; the interaction enhances sumoylation and represses the transactivational activity in a sumoylation-independent manner. Interacts with LMCD1. Interacts with CREBBP; the interaction stimulates acetylation and transcriptional activity in vivo. Interacts with EP300. Erythrocytes. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: DNA-binding; Transcription factor

Chromosomal Location of Human Ortholog: Xq12

Cellular Component: nucleus; transcription factor complex; transcriptional repressor complex

Molecular Function: chromatin binding; chromatin DNA binding; DNA binding; DNA binding transcription factor activity; DNA binding, bending; p53 binding; sequence-specific DNA binding; zinc ion binding

Biological Process: basophil differentiation; cell development; cell-cell signaling; elevation of cytosolic calcium ion concentration; embryonic hemopoiesis; eosinophil differentiation; erythrocyte development; erythrocyte differentiation; homeostasis of number of cells within a tissue; in utero embryonic development; male gonad development; megakaryocyte differentiation; myeloid cell differentiation; negative regulation of apoptosis; negative regulation of bone mineralization; negative regulation of cell proliferation; negative regulation of transcription from RNA polymerase II promoter; organ regeneration; platelet formation; positive regulation of erythrocyte differentiation; positive regulation of mast cell degranulation; positive regulation of osteoblast proliferation; positive regulation of peptidyl-tyrosine phosphorylation; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-templated; regulation of transcription, DNA-templated; Sertoli cell development; transcription from RNA polymerase II promoter

Research Articles on GATA1

1. suggest that the chromatin environment in the vicinity of GATA1 and NF-E2 plays a critical role for alpha2B-AR expression during fetal life

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with GATA1 elisa kit

Products by Pathway	Pathway Diagram
Delta-Notch Signaling Pathway antibodies	Delta-Notch Signaling Pathway Diagram
IL-3 Signaling Pathway antibodies	IL-3 Signaling Pathway Diagram

Diseases associated with GATA1 elisa kit

Disease Name	Pubmed Publications
Leukemia Antibodies	>339 publications with GATA1 and Leukemia
Neoplasms Antibodies	>292 publications with GATA1 and Neoplasms
Hematologic Diseases Antibodies	>268 publications with GATA1 and Hematologic Diseases
Leukemia, Myeloid, Acute Antibodies	>216 publications with GATA1 and Leukemia, Myeloid, Acute
Bone Marrow Diseases Antibodies	>183 publications with GATA1 and Bone Marrow Diseases
Down Syndrome Antibodies	>139 publications with GATA1 and Down Syndrome
Nervous System Diseases Antibodies	>125 publications with GATA1 and Nervous System Diseases
Anemia Antibodies	>113 publications with GATA1 and Anemia
Thrombocytopenia Antibodies	>58 publications with GATA1 and Thrombocytopenia
Disease Models, Animal Antibodies	>33 publications with GATA1 and Disease Models, Animal

Organs/Tissues associated with GATA1 elisa kit

Organ/Tissue Name	Pubmed Publications
Liver Antibodies	>81 publications with GATA1 and Liver
Embryonic Tissue Antibodies	>74 publications with GATA1 and Embryonic Tissue
Heart Antibodies	>34 publications with GATA1 and Heart
Placenta Antibodies	>5 publications with GATA1 and Placenta