Major Facilitator Superfamily Domain-Containing Protein 1 (MFSD1) ELISA Kit for Chicken

MFSD1, Belongs to the major facilitator superfamily. The major facilitator superfamily (MFS) is one of the two largest families of membrane transporters found on Earth. It is present ubiquitously in bacteria, archaea, and eukarya and includes members that can function by solute uniport, solute/cation symport, solute/cation antiport and/or solute/solute antiport with inwardly and/or outwardly directed polarity. Phylogenetic analyses revealed the occurrence of 17 distinct families within the MFS, each of which generally transports a single class of compounds. Compounds transported by MFS permeases include simple sugars, oligosaccharides, inositols, drugs, amino acids, nucleosides, organophosphate esters, Krebs cycle metabolites, and a large variety of organic and inorganic anions and cations.

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate MFSD1 in samples. An antibody specific for MFSD1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMFSD1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MFSD1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MFSD1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Small volumes of MFSD1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS283977 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Major facilitator superfamily domain-containing protein 1 (MFSD1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing MFSD1. The ELISA analytical biochemical technique of the MBS283977 kit is based on MFSD1 antibody-MFSD1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect MFSD1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, MFSD1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).