Guinea Pig HTR2A ELISA Kit – Cat# MBS284059

5-Hydroxytryptamine Receptor 2a (HTR2A) ELISA Kit for Guinea Pig

Name: Guinea Pig HTR2A ELISA Kit; 5-Hydroxytryptamine Receptor 2a; MyBioSource
Brand: MyBioSource
SKU: MBS284059
Price: 545 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS284059

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate HTR2A in samples. An antibody specific for HTR2A has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyHTR2A present is bound by the… More details

$545 / 48 Tests +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Request Current Manual

Product Overview

Online Datasheet in PDF

Product Name

5-hydroxytryptamine receptor 2A (HTR2A), ELISA Kit

Full Product Name

Guinea pig 5-hydroxytryptamine receptor 2A (HTR2A) ELISA Kit

Product Gene Name

HTR2A elisa kit

Product Synonym Names

RP11-147L20.2; 5-HT2A; HTR2; 5-HT2 receptor|5-hydroxytryptamine receptor 2A|serotonin 5-HT-2A receptor

Species Reactivity

Guinea pig

Assay Type

Sandwich

Samples

Serum, Plasma, Other biological fluids

Specificity

This assay has high sensitivity and excellent specificity for detection of Guinea pig HTR2A. No significant cross-reactivity or interference between Guinea pig HTR2A and analogues was observed.

Preparation and Storage

Store at 2-8 degree C.

Molecular Weight

27,698 Da

3D Structure

ModBase 3D Structure for P35382

Sample Volume

1-200 uL

Precision

Intra-assay Precision (Precision within an assay)
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%) = SD/meanX100
Intra-Assay: CV
Inter-Assay: CV

Detection Wavelength

450 nm

ISO Certification

Manufactured in an ISO 9001:2015 Certified Laboratory.

Other Notes

Small volumes of HTR2A elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for HTR2A purchase

MBS284059 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the 5-hydroxytryptamine receptor 2A (HTR2A) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing HTR2A. The ELISA analytical biochemical technique of the MBS284059 kit is based on HTR2A antibody-HTR2A antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect HTR2A antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, HTR2A. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for HTR2A. It may not necessarily be applicable to this product.

NCBI GI #

543726

NCBI Accession #

P35382.1

UniProt Primary Accession #

P35382

NCBI Official Full Name

5-hydroxytryptamine receptor 2A

UniProt Protein Name

5-hydroxytryptamine receptor 2A

UniProt Synonym Protein Names

Serotonin receptor 2A

Protein Family

5-hydroxytryptamine receptor

UniProt Gene Name

HTR2A

UniProt Synonym Gene Names

5-HT-2; 5-HT-2A

UniProt Comments for HTR2A

G-protein coupled receptor for 5-hydroxytryptamine (serotonin). Also functions as a receptor for various drugs and psychoactive substances, including mescaline, psilocybin, 1-(2,5-dimethoxy-4-iodophenyl)-2-aminopropane (DOI) and lysergic acid diethylamide (LSD). Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors. Beta-arrestin family members inhibit signaling via G proteins and mediate activation of alternative signaling pathways. Signaling activates phospholipase C and a phosphatidylinositol-calcium second messenger system that modulates the activity of phosphatidylinositol 3-kinase and promotes the release of Ca2+ ions from intracellular stores. Affects neural activity, perception, cognition and mood. Plays a role in the regulation of behavior, including responses to anxiogenic situations and psychoactive substances. Plays a role in intestinal smooth muscle contraction, and may play a role in arterial vasoconstriction.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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