Kaiso (ZBTB33) ELISA Kit for Human

ZBTB33 encodes a transcriptional regulator with bimodal DNA-binding specificity, which binds to methylated CGCG and also to the non-methylated consensus KAISO-binding site TCCTGCNA.
The protein contains an N-terminal POZ/BTB domain and 3 C-terminal zinc finger motifs. It recruits the N-CoR repressor complex to promote histone deacetylation and the formation of repressive chromatin structures in target gene promoters. It may contribute to the repression of target genes of the Wnt signaling pathway, and may also activate transcription of a subset of target genes by the recruitment of catenin delta-2 (CTNND2). Its interaction with catenin delta-1 (CTNND1) inhibits binding to both methylated and non-methylated DNA.

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate ZBTB33 in samples. An antibody specific for ZBTB33 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyZBTB33 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ZBTB33 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ZBTB33 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Small volumes of ZBTB33 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS284180 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Kaiso (ZBTB33) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing ZBTB33. The ELISA analytical biochemical technique of the MBS284180 kit is based on ZBTB33 antibody-ZBTB33 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect ZBTB33 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, ZBTB33. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).