Interleukin 28a (IL-28A) ELISA Kit for Human

Name: Human IL-28A ELISA Kit; Interleukin 28a; MyBioSource
Brand: MyBioSource
SKU: MBS284190
Price: 370 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS284190

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate IL28A in samples. An antibody specific for IL28A has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyIL28A present is bound by the… More details

$370 / 48 Tests +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

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Product Overview

Online Datasheet in PDF

Product Name

Interleukin 28A (IL-28A), ELISA Kit

Full Product Name

Human Interleukin 28A (IL-28A/IFN-lambda2) ELISA Kit

Product Gene Name

IL-28A elisa kit

Product Synonym Names

IFNL2; IL-28A; interferon lambda 2|interferon-lambda 2|interleukin 28A

Species Reactivity

Human

Assay Type

Sandwich

Samples

Serum, Plasma, Other biological fluids

Specificity

This assay has high sensitivity and excellent specificity for detection of Human IL28A. No significant cross-reactivity or interference between Human IL28A and analogues was observed.

Preparation and Storage

Store at 2-8 degree C.

Molecular Weight

22,288 Da

OMIM

AY129148 mRNA

3D Structure

ModBase 3D Structure for Q8IZJ0

Sample Volume

1-200 uL

Precision

Intra-assay Precision (Precision within an assay)
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%) = SD/meanX100
Intra-Assay: CV
Inter-Assay: CV

Detection Wavelength

450 nm

ISO Certification

Manufactured in an ISO 9001:2015 Certified Laboratory.

Other Notes

Small volumes of IL-28A elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for IL-28A purchase

MBS284190 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Interleukin 28A (IL-28A) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing IL-28A. The ELISA analytical biochemical technique of the MBS284190 kit is based on IL-28A antibody-IL-28A antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect IL-28A antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, IL-28A. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for IL-28A. It may not necessarily be applicable to this product.

NCBI GI #

26024321

NCBI GeneID

282616

NCBI Accession #

NP_742150.1

UniProt Primary Accession #

Q8IZJ0

UniProt Secondary Accession #

Q45KQ8; Q6VN55; Q8IWL7

UniProt Related Accession #

Q8IZJ0

GenBank Nucleotide #

NM_172138.1

NCBI Official Full Name

interferon lambda-2

NCBI Official Synonym Full Names

interferon lambda 2

NCBI Official Symbol

IFNL2

NCBI Official Synonym Symbols

IL28A; IL-28A

UniProt Protein Name

Interferon lambda-2

UniProt Synonym Protein Names

Cytokine Zcyto20; Interleukin-28A; IL-28A

Protein Family

Interferon

UniProt Gene Name

IFNL2

UniProt Synonym Gene Names

IL28A; ZCYTO20; IFN-lambda-2; IL-28A

NCBI Protein Information

interferon lambda-2

NCBI Summary for IL-28A

This gene encodes a cytokine distantly related to type I interferons and the IL-10 family. This gene, interleukin 28B (IL28B), and interleukin 29 (IL29) are three closely related cytokine genes that form a cytokine gene cluster on a chromosomal region mapped to 19q13. Expression of the cytokines encoded by the three genes can be induced by viral infection. All three cytokines have been shown to interact with a heterodimeric class II cytokine receptor that consists of interleukin 10 receptor, beta (IL10RB) and interleukin 28 receptor, alpha (IL28RA). [provided by RefSeq, Jul 2008]

UniProt Comments for IL-28A

IL28A: Cytokine with immunomodulatory activity. Up-regulates MHC class I antigen expression. Displays potent antiviral activity. Also displays antitumor activity. Ligand for the heterodimeric class II cytokine receptor composed of IL10RB and IL28RA. The ligand/receptor complex seems to signal through the Jak-STAT pathway. Belongs to the IL-28/IL-29 family.

Protein type: Cytokine; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 19q13.2

Cellular Component: extracellular space; intracellular

Molecular Function: cytokine activity; receptor binding

Biological Process: defense response to virus; innate immune response; JAK-STAT cascade; mucosal immune response; positive regulation of immune response; regulation of T cell activation

Research Articles on IL-28A

1. NS of severe fever with thrombocytopenia syndrome virus inhibited the activity of IFN-alpha1, IFN-beta, IFN-lambda1 and IFN-lambda2 through inhibition of STAT1 phosphorylation.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with IL-28A elisa kit

Products by Pathway	Pathway Diagram
Cytokine-cytokine Receptor Interaction Pathway antibodies	Cytokine-cytokine Receptor Interaction Pathway Diagram
Cytokine-cytokine Receptor Interaction Pathway antibodies	Cytokine-cytokine Receptor Interaction Pathway Diagram
Jak-STAT Signaling Pathway antibodies	Jak-STAT Signaling Pathway Diagram
Jak-STAT Signaling Pathway antibodies	Jak-STAT Signaling Pathway Diagram
Type III Interferon Signaling Pathway antibodies	Type III Interferon Signaling Pathway Diagram