Carbonic Anhydrase 9 (CA9) ELISA Kit for Cat

Name: Cat CA9 ELISA Kit; Carbonic Anhydrase 9; MyBioSource
Brand: MyBioSource
SKU: MBS284342
Price: 545 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS284342

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate CA9 in samples. An antibody specific for CA9 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyCA9 present is bound by the… More details

$545 / 48 Tests +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

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Product Overview

Online Datasheet in PDF

Product Name

Carbonic Anhydrase 9 (CA9), ELISA Kit

Full Product Name

Cat Carbonic Anhydrase 9 (CA9) ELISA Kit

Product Gene Name

CA9 elisa kit

Product Synonym Names

CA-IX; CAIX; MN; pMW1; P54/58N; Carbonic Dehydratase; Renal cell carcinoma-associated antigen G250; RCC-Associated Protein G250; Carbonate dehydratase IX

Species Reactivity

Cat

Assay Type

Sandwich

Samples

Serum, Plasma, Other biological fluids

Specificity

This assay has high sensitivity and excellent specificity for detection of Cat CA9. No significant cross-reactivity or interference between Cat CA9 and analogues was observed.

Preparation and Storage

Store at 2-8 degree C.

Molecular Weight

49,698 Da

OMIM

603179

Sample Volume

1-200 uL

Precision

Intra-assay Precision (Precision within an assay)
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%) = SD/meanX100
Intra-Assay: CV
Inter-Assay: CV

Detection Wavelength

450 nm

ISO Certification

Manufactured in an ISO 9001:2015 Certified Laboratory.

Other Notes

Small volumes of CA9 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CA9 purchase

MBS284342 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Carbonic Anhydrase 9 (CA9) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing CA9. The ELISA analytical biochemical technique of the MBS284342 kit is based on CA9 antibody-CA9 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect CA9 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, CA9. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for CA9. It may not necessarily be applicable to this product.

NCBI GI #

169636420

NCBI GeneID

768

NCBI Accession #

NP_001207.2

UniProt Secondary Accession #

Q5T4R1

UniProt Related Accession #

Q16790

GenBank Nucleotide #

NM_001216.2

NCBI Official Full Name

carbonic anhydrase 9

NCBI Official Synonym Full Names

carbonic anhydrase IX

NCBI Official Symbol

CA9

NCBI Official Synonym Symbols

MN; CAIX

UniProt Protein Name

Carbonic anhydrase 9

UniProt Synonym Protein Names

Carbonate dehydratase IX; Carbonic anhydrase IX; CA-IX; CAIX; Membrane antigen MN; P54/58N; Renal cell carcinoma-associated antigen G250; RCC-associated antigen G250; pMW1

Protein Family

Carbonic anhydrase

UniProt Gene Name

CA9

UniProt Synonym Gene Names

G250; MN; CA-IX; CAIX; RCC-associated antigen G250

UniProt Entry Name

CAH9_HUMAN

NCBI Protein Information

carbonic anhydrase 9

NCBI Summary for CA9

Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. They show extensive diversity in tissue distribution and in their subcellular localization. CA IX is a transmembrane protein and is one of only two tumor-associated carbonic anhydrase isoenzymes known. It is expressed in all clear-cell renal cell carcinoma, but is not detected in normal kidney or most other normal tissues. It may be involved in cell proliferation and transformation. This gene was mapped to 17q21.2 by fluorescence in situ hybridization, however, radiation hybrid mapping localized it to 9p13-p12. [provided by RefSeq, Jun 2014]

UniProt Comments for CA9

CA9: Reversible hydration of carbon dioxide. Participates in pH regulation. May be involved in the control of cell proliferation and transformation. Appears to be a novel specific biomarker for a cervical neoplasia. Forms oligomers linked by disulfide bonds. By hypoxia. Expressed primarily in carcinoma cells lines. Expression is restricted to very few normal tissues and the most abundant expression is found in the epithelial cells of gastric mucosa. Inhibited by coumarins, saccharin, sulfonamide derivatives such as acetazolamide (AZA) and Foscarnet (phosphonoformate trisodium salt). Belongs to the alpha-carbonic anhydrase family.

Protein type: EC 4.2.1.1; Lyase; Nucleolus; Energy Metabolism - nitrogen; Membrane protein, integral

Chromosomal Location of Human Ortholog: 9p13.3

Cellular Component: basolateral plasma membrane; integral to membrane; microvillus membrane; nucleolus; plasma membrane

Molecular Function: carbonate dehydratase activity; zinc ion binding

Biological Process: bicarbonate transport; morphogenesis of an epithelium; one-carbon compound metabolic process; response to drug; response to testosterone stimulus; secretion

Research Articles on CA9

1. A strong positive correlation between the mRNA expression levels of HIF-2alpha, CA9, VEGF, GLUT-1 and OPN suggests a specific hypoxia-associated profile of mRNA expression in glioblastoma multiforme

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with CA9 elisa kit

Reference Product	PubMed Publications
HIF1A elisa kit	>169 publications with CA9 and HIF1A
EGFR elisa kit	>29 publications with CA9 and EGFR
CTNNB1 elisa kit	>5 publications with CA9 and CTNNB1
TP53 elisa kit	>3 publications with CA9 and TP53
EP300 elisa kit	>3 publications with CA9 and EP300
ARNT elisa kit	>3 publications with CA9 and ARNT
SLC4A4 elisa kit	>2 publications with CA9 and SLC4A4
HIF3A elisa kit	>2 publications with CA9 and HIF3A

Pathways associated with CA9 elisa kit

Products by Pathway	Pathway Diagram
Cellular Response To Hypoxia Pathway antibodies	Cellular Response To Hypoxia Pathway Diagram
Cellular Responses To Stress Pathway antibodies	Cellular Responses To Stress Pathway Diagram
HIF-1-alpha Transcription Factor Network Pathway antibodies	HIF-1-alpha Transcription Factor Network Pathway Diagram
Metabolism Pathway antibodies	Metabolism Pathway Diagram
Nitrogen Metabolism Pathway antibodies	Nitrogen Metabolism Pathway Diagram
Nitrogen Metabolism Pathway antibodies	Nitrogen Metabolism Pathway Diagram
Regulation Of Hypoxia-inducible Factor (HIF) By Oxygen Pathway antibodies	Regulation Of Hypoxia-inducible Factor (HIF) By Oxygen Pathway Diagram
Regulation Of Gene Expression By Hypoxia-inducible Factor Pathway antibodies	Regulation Of Gene Expression By Hypoxia-inducible Factor Pathway Diagram
Reversible Hydration Of Carbon Dioxide Pathway antibodies	Reversible Hydration Of Carbon Dioxide Pathway Diagram

Diseases associated with CA9 elisa kit

Disease Name	Pubmed Publications
Adenocarcinoma Antibodies	>264 publications with CA9 and Adenocarcinoma
Kidney Diseases Antibodies	>197 publications with CA9 and Kidney Diseases
Neoplasm Metastasis Antibodies	>146 publications with CA9 and Neoplasm Metastasis
Breast Neoplasms Antibodies	>88 publications with CA9 and Breast Neoplasms
Anoxia Antibodies	>81 publications with CA9 and Anoxia
Neoplasms, Experimental Antibodies	>74 publications with CA9 and Neoplasms, Experimental
Necrosis Antibodies	>56 publications with CA9 and Necrosis
Neoplasm Invasiveness Antibodies	>54 publications with CA9 and Neoplasm Invasiveness
Lung Neoplasms Antibodies	>53 publications with CA9 and Lung Neoplasms
Nervous System Diseases Antibodies	>42 publications with CA9 and Nervous System Diseases

Organs/Tissues associated with CA9 elisa kit

Organ/Tissue Name	Pubmed Publications
Kidney Antibodies	>205 publications with CA9 and Kidney
Blood Antibodies	>159 publications with CA9 and Blood
Lung Antibodies	>64 publications with CA9 and Lung
Brain Antibodies	>36 publications with CA9 and Brain
Cervix Antibodies	>30 publications with CA9 and Cervix
Bone Antibodies	>25 publications with CA9 and Bone
Pancreas Antibodies	>14 publications with CA9 and Pancreas
Stomach Antibodies	>13 publications with CA9 and Stomach
Uterus Antibodies	>10 publications with CA9 and Uterus
Intestine Antibodies	>10 publications with CA9 and Intestine