Interleukin-1 Receptor-Associated Kinase-Like 2 (IRAK2) ELISA Kit for Rat

Name: Rat IRAK2 ELISA Kit; Interleukin-1 Receptor-Associated Kinase-Like 2; MyBioSource
Brand: MyBioSource
SKU: MBS284399
Price: 495 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS284399

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate IRAK2 in samples. An antibody specific for IRAK2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyIRAK2 present is bound by the… More details

$495 / 48 Tests +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

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Product Overview

Online Datasheet in PDF

Product Name

Interleukin-1 receptor-associated kinase-like 2 (IRAK2), ELISA Kit

Full Product Name

Rat Interleukin-1 receptor-associated kinase-like 2 (IRAK2) ELISA Kit

Product Gene Name

IRAK2 elisa kit

Product Synonym Names

IRAK-2; MGC150550; interleukin-1 receptor associated kinase-2

Species Reactivity

Rat

Assay Type

Sandwich

Samples

Serum, Plasma, Other biological fluids

Specificity

This assay has high sensitivity and excellent specificity for detection of Rat IRAK2. No significant cross-reactivity or interference between Rat IRAK2 and analogues was observed.

Preparation and Storage

Store at 2-8 degree C.

Molecular Weight

69,237 Da

3D Structure

ModBase 3D Structure for Q4QQS0

Sample Volume

1-200 uL

Precision

Intra-assay Precision (Precision within an assay)
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%) = SD/meanX100
Intra-Assay: CV
Inter-Assay: CV

Detection Wavelength

450 nm

ISO Certification

Manufactured in an ISO 9001:2015 Certified Laboratory.

Other Notes

Small volumes of IRAK2 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for IRAK2 purchase

MBS284399 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Interleukin-1 receptor-associated kinase-like 2 (IRAK2) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing IRAK2. The ELISA analytical biochemical technique of the MBS284399 kit is based on IRAK2 antibody-IRAK2 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect IRAK2 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, IRAK2. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for IRAK2. It may not necessarily be applicable to this product.

NCBI GI #

70794740

NCBI GeneID

362418

NCBI Accession #

NP_001020593.1

UniProt Primary Accession #

Q4QQS0

UniProt Related Accession #

Q4QQS0

GenBank Nucleotide #

NM_001025422.1

NCBI Official Full Name

interleukin-1 receptor-associated kinase-like 2

NCBI Official Synonym Full Names

interleukin-1 receptor-associated kinase 2

NCBI Official Symbol

Irak2

UniProt Protein Name

Interleukin-1 receptor-associated kinase-like 2

Protein Family

Interleukin-1 receptor-associated kinase

UniProt Gene Name

Irak2

UniProt Synonym Gene Names

IRAK-2

NCBI Protein Information

interleukin-1 receptor-associated kinase-like 2

UniProt Comments for IRAK2

IRAK2: Binds to the IL-1 type I receptor following IL-1 engagement, triggering intracellular signaling cascades leading to transcriptional up-regulation and mRNA stabilization. Interacts with MYD88. IL-1 stimulation leads to the formation of a signaling complex which dissociates from the IL-1 receptor following the binding of PELI1. Expressed in spleen, thymus, prostate, lung, liver, skeletal muscle, kidney, pancreas and peripheral blood leukocytes. Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. Pelle subfamily.

Protein type: IRAK family; Kinase, protein; Protein kinase, Ser/Thr (non-receptor); Protein kinase, TKL; TKL group

Chromosomal Location of Human Ortholog: 4q42

Cellular Component: cytoplasm; cytosol; nucleus

Molecular Function: ATP binding; protein heterodimerization activity; protein homodimerization activity; protein serine/threonine kinase activity

Biological Process: activation of NF-kappaB-inducing kinase; I-kappaB kinase/NF-kappaB signaling; inflammatory response; lipopolysaccharide-mediated signaling pathway; MyD88-dependent toll-like receptor signaling pathway; negative regulation of apoptosis; negative regulation of NF-kappaB transcription factor activity; negative regulation of transcription factor activity; positive regulation of NF-kappaB transcription factor activity; protein amino acid phosphorylation; protein autophosphorylation; regulation of cytokine-mediated signaling pathway; toll-like receptor signaling pathway

Research Articles on IRAK2

1. Elevated circulating IL-1beta contributed to promote ER stress- induced myocytes apoptosis by affecting IRAK-2/CHOP pathway in diabetic cardiomyopathy.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with IRAK2 elisa kit

Reference Product	PubMed Publications
Irak1 elisa kit	>278 publications with IRAK2 and Irak1
Myd88 elisa kit	>261 publications with IRAK2 and Myd88
Traf6 elisa kit	>257 publications with IRAK2 and Traf6
Irak4 elisa kit	>176 publications with IRAK2 and Irak4
Tollip elisa kit	>35 publications with IRAK2 and Tollip
Il1b elisa kit	>7 publications with IRAK2 and Il1b
Il1r1 elisa kit	>3 publications with IRAK2 and Il1r1
Il1a elisa kit	>2 publications with IRAK2 and Il1a
Map3k7ip2 elisa kit	>1 publications with IRAK2 and Map3k7ip2

Pathways associated with IRAK2 elisa kit

Products by Pathway	Pathway Diagram
Activated TLR4 Signalling Pathway antibodies	Activated TLR4 Signalling Pathway Diagram
Apoptosis Pathway antibodies	Apoptosis Pathway Diagram
Apoptosis Pathway antibodies	Apoptosis Pathway Diagram
Cytokine Signaling In Immune System Pathway antibodies	Cytokine Signaling In Immune System Pathway Diagram
IL-1 Signaling Pathway antibodies	IL-1 Signaling Pathway Diagram
IRAK2 Mediated Activation Of TAK1 Complex Pathway antibodies	IRAK2 Mediated Activation Of TAK1 Complex Pathway Diagram
IRAK2 Mediated Activation Of TAK1 Complex Upon TLR7/8 Or 9 Stimulation Pathway antibodies	IRAK2 Mediated Activation Of TAK1 Complex Upon TLR7/8 Or 9 Stimulation Pathway Diagram
Immune System Pathway antibodies	Immune System Pathway Diagram
Innate Immune System Pathway antibodies	Innate Immune System Pathway Diagram
Interleukin-1 Signaling Pathway antibodies	Interleukin-1 Signaling Pathway Diagram

Diseases associated with IRAK2 elisa kit

Disease Name	Pubmed Publications
Necrosis Antibodies	>316 publications with IRAK2 and Necrosis
Inflammation Antibodies	>283 publications with IRAK2 and Inflammation
Disease Models, Animal Antibodies	>48 publications with IRAK2 and Disease Models, Animal
Lung Diseases Antibodies	>44 publications with IRAK2 and Lung Diseases
Nervous System Diseases Antibodies	>29 publications with IRAK2 and Nervous System Diseases
Skin Diseases Antibodies	>25 publications with IRAK2 and Skin Diseases
Atherosclerosis Antibodies	>25 publications with IRAK2 and Atherosclerosis
Lung Injury Antibodies	>20 publications with IRAK2 and Lung Injury
Liver Diseases Antibodies	>18 publications with IRAK2 and Liver Diseases
Pneumonia Antibodies	>15 publications with IRAK2 and Pneumonia

Organs/Tissues associated with IRAK2 elisa kit

Organ/Tissue Name	Pubmed Publications
Lung Antibodies	>51 publications with IRAK2 and Lung
Brain Antibodies	>24 publications with IRAK2 and Brain
Spleen Antibodies	>22 publications with IRAK2 and Spleen
Prostate Antibodies	>5 publications with IRAK2 and Prostate
Connective Tissue Antibodies	>4 publications with IRAK2 and Connective Tissue
Placenta Antibodies	>3 publications with IRAK2 and Placenta