Interleukin 1 Receptor Antagonist (IL-1ra) ELISA Kit for Mouse

Name: Mouse IL-1ra ELISA Kit; Interleukin 1 Receptor Antagonist; MyBioSource
Brand: MyBioSource
SKU: MBS284542
Price: 545 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS284542

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate IL1RA in samples. An antibody specific for IL1RA has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyIL1RA present is bound by the… More details

$545 / 48 Tests +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

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Product Overview

Online Datasheet in PDF

Product Name

Interleukin 1 receptor antagonist (IL-1ra), ELISA Kit

Full Product Name

Mouse Interleukin 1 receptor antagonist (IL-1ra) ELISA Kit

Product Gene Name

IL-1ra elisa kit

Product Synonym Names

DIRA; ICIL-1RA; IL-1ra3; IL1F3; IL1RA; IRAP; MGC10430; MVCD4; IL1RN (IL1F3)|OTTHUMP00000203730|intracellular IL-1 receptor antagonist type II|intracellular interleukin-1 receptor antagonist (icIL-1r

Species Reactivity

Mouse

Assay Type

Sandwich

Samples

Serum, Plasma, Other biological fluids

Specificity

This assay has high sensitivity and excellent specificity for detection of Mouse IL1RA. No significant cross-reactivity or interference between Mouse IL1RA and analogues was observed.

Preparation and Storage

Store at 2-8 degree C.

Molecular Weight

17,996 Da

3D Structure

ModBase 3D Structure for P25085

Sample Volume

1-200 uL

Precision

Intra-assay Precision (Precision within an assay)
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%) = SD/meanX100
Intra-Assay: CV
Inter-Assay: CV

Detection Wavelength

450 nm

ISO Certification

Manufactured in an ISO 9001:2015 Certified Laboratory.

Other Notes

Small volumes of IL-1ra elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for IL-1ra purchase

MBS284542 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Interleukin 1 receptor antagonist (IL-1ra) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing IL-1ra. The ELISA analytical biochemical technique of the MBS284542 kit is based on IL-1ra antibody-IL-1ra antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect IL-1ra antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, IL-1ra. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for IL-1ra. It may not necessarily be applicable to this product.

NCBI GI #

89257344

NCBI GeneID

16181

NCBI Accession #

NP_001034790.1

UniProt Primary Accession #

P25085

UniProt Secondary Accession #

O70207

UniProt Related Accession #

P25085

GenBank Nucleotide #

NM_001039701.3

NCBI Official Full Name

interleukin-1 receptor antagonist protein isoform 2

NCBI Official Synonym Full Names

interleukin 1 receptor antagonist

NCBI Official Symbol

Il1rn

NCBI Official Synonym Symbols

IL-1ra; F630041P17Rik

UniProt Protein Name

Interleukin-1 receptor antagonist protein

UniProt Synonym Protein Names

IL1 inhibitor

Protein Family

Interleukin-1 receptor antagonist protein

UniProt Gene Name

Il1rn

UniProt Synonym Gene Names

Il-1ra; IL-1RN; IL-1ra; IRAP

NCBI Protein Information

interleukin-1 receptor antagonist protein

UniProt Comments for IL-1ra

IL1RN: Inhibits the activity of interleukin-1 by binding to receptor IL1R1 and preventing its association with the coreceptor IL1RAP for signaling. Has no interleukin-1 like activity. Binds functional interleukin-1 receptor IL1R1 with greater affinity than decoy receptor IL1R2; however, the physiological relevance of the latter association is unsure. The intracellular form of IL1RN is predominantly expressed in epithelial cells. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Cytokine; Secreted; Secreted, signal peptide; Vesicle

Chromosomal Location of Human Ortholog: 2 A3|2 16.36 cM

Cellular Component: cytoplasm; extracellular region; extracellular space; vesicle

Molecular Function: cytokine activity; interleukin-1 receptor antagonist activity; interleukin-1 receptor binding; interleukin-1 Type I receptor antagonist activity; interleukin-1 Type II receptor antagonist activity; interleukin-1, Type I receptor binding; interleukin-1, Type II receptor binding

Biological Process: acute-phase response; cytokine-mediated signaling pathway; fever; immune response; inflammatory response; insulin secretion; lipid metabolic process; memory; negative regulation of apoptosis; negative regulation of cell migration; negative regulation of glutamate secretion; negative regulation of heterotypic cell-cell adhesion; negative regulation of membrane potential; positive regulation of JUN kinase activity; response to glucocorticoid stimulus; sensory perception of pain

Research Articles on IL-1ra

1. Following vasectomy, IL1alpha, IL1beta, IL1ra, IL10, and TNF-alpha may mediate immune reaction in whole epididymis, whereas IL6 and TGF-beta1 may mediate regionally different immune response primarily in the lower part of epididymis.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with IL-1ra elisa kit

Products by Pathway	Pathway Diagram
Cytokine Signaling In Immune System Pathway antibodies	Cytokine Signaling In Immune System Pathway Diagram
IL-1 Signaling Pathway antibodies	IL-1 Signaling Pathway Diagram
Immune System Pathway antibodies	Immune System Pathway Diagram
Interleukin-1 Signaling Pathway antibodies	Interleukin-1 Signaling Pathway Diagram
Signaling By Interleukins Pathway antibodies	Signaling By Interleukins Pathway Diagram

Diseases associated with IL-1ra elisa kit

Disease Name	Pubmed Publications
Necrosis Antibodies	>778 publications with IL-1ra and Necrosis
Inflammation Antibodies	>697 publications with IL-1ra and Inflammation
Arthritis, Rheumatoid Antibodies	>302 publications with IL-1ra and Arthritis, Rheumatoid
Cardiovascular Diseases Antibodies	>190 publications with IL-1ra and Cardiovascular Diseases
Disease Models, Animal Antibodies	>165 publications with IL-1ra and Disease Models, Animal
Skin Diseases Antibodies	>126 publications with IL-1ra and Skin Diseases
Lung Diseases Antibodies	>111 publications with IL-1ra and Lung Diseases
Pain Antibodies	>82 publications with IL-1ra and Pain
Kidney Diseases Antibodies	>80 publications with IL-1ra and Kidney Diseases
Heart Diseases Antibodies	>76 publications with IL-1ra and Heart Diseases

Organs/Tissues associated with IL-1ra elisa kit

Organ/Tissue Name	Pubmed Publications
Blood Antibodies	>1072 publications with IL-1ra and Blood
Skin Antibodies	>127 publications with IL-1ra and Skin
Bone Marrow Antibodies	>67 publications with IL-1ra and Bone Marrow
Stomach Antibodies	>51 publications with IL-1ra and Stomach
Connective Tissue Antibodies	>50 publications with IL-1ra and Connective Tissue
Spleen Antibodies	>43 publications with IL-1ra and Spleen
Intestine Antibodies	>40 publications with IL-1ra and Intestine
Eye Antibodies	>38 publications with IL-1ra and Eye
Vagina Antibodies	>11 publications with IL-1ra and Vagina
Thymus Antibodies	>7 publications with IL-1ra and Thymus