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Cytokeratin 17 (CK-17) ELISA Kit for Human

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS284545

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate KRT17 in samples. An antibody specific for KRT17 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyKRT17 present is bound by the… More details

$495 / 48 Tests +1 FREE 8GB USB

Product Documentation

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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Cytokeratin 17 (CK-17), ELISA Kit
Full Product Name
Human Cytokeratin 17 (CK-17) ELISA Kit
Product Gene Name
CK-17 elisa kit
Product Synonym Names
K17; PC; PC2; PCHC1; cytokeratin-17
Species Reactivity
Human
Assay Type
Sandwich
Samples
Serum, Plasma, Other biological fluids
Specificity
This assay has high sensitivity and excellent specificity for detection of Human KRT17. No significant cross-reactivity or interference between Human KRT17 and analogues was observed.
Preparation and Storage
Store at 2-8 degree C.
Molecular Weight
48,106 Da
OMIM
Z19574 Genomic DNA
3D Structure
ModBase 3D Structure for Q04695
Sample Volume
1-200 uL
Precision
Intra-assay Precision (Precision within an assay)
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%) = SD/meanX100
Intra-Assay: CV
Inter-Assay: CV
Detection Wavelength
450 nm
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.

Other Notes

Small volumes of CK-17 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CK-17 purchase

MBS284545 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Cytokeratin 17 (CK-17) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing CK-17. The ELISA analytical biochemical technique of the MBS284545 kit is based on CK-17 antibody-CK-17 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect CK-17 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, CK-17. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for CK-17. It may not necessarily be applicable to this product.

NCBI GI #
4557701
NCBI GeneID
3872
NCBI Accession #
NP_000413.1
UniProt Primary Accession #
Q04695
UniProt Secondary Accession #
Q6IP98; Q8N1P6; A5Z1M9; A5Z1N0; A5Z1N1; A5Z1N2; A6NDV6; A6NKQ2
UniProt Related Accession #
Q04695
GenBank Nucleotide #
NM_000422.2
NCBI Official Full Name
keratin, type I cytoskeletal 17
NCBI Official Synonym Full Names
keratin 17
NCBI Official Symbol
KRT17
NCBI Official Synonym Symbols
PC; K17; PC2; 39.1; CK-17; PCHC1
UniProt Protein Name
Keratin, type I cytoskeletal 17
UniProt Synonym Protein Names
39.1; Cytokeratin-17; CK-17; Keratin-17; K17
Protein Family
Keratin
UniProt Gene Name
KRT17
UniProt Synonym Gene Names
CK-17; K17
NCBI Protein Information
keratin, type I cytoskeletal 17

NCBI Summary for CK-17

This gene encodes the type I intermediate filament chain keratin 17, expressed in nail bed, hair follicle, sebaceous glands, and other epidermal appendages. Mutations in this gene lead to Jackson-Lawler type pachyonychia congenita and steatocystoma multiplex. [provided by RefSeq, Aug 2008]

UniProt Comments for CK-17

K17: a type I cytoskeletal keratin. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. There are two types of cytoskeletal and microfibrillar keratin: type I (acidic; 40-55 kDa) [K9 to K20] and type II (neutral to basic; 56-70 kDa) [K1 to K8]. Both a basic and an acidic keratin are required for filament assembly. Generally associates with K6.

Protein type: Cytoskeletal

Chromosomal Location of Human Ortholog: 17q21.2

Cellular Component: cell periphery; cytosol; extracellular exosome; intermediate filament; intermediate filament cytoskeleton

Molecular Function: MHC class II protein binding; MHC class II receptor activity; protein binding; structural constituent of cytoskeleton

Biological Process: cornification; epidermis development; hair follicle morphogenesis; intermediate filament organization; keratinization; positive regulation of cell growth; positive regulation of hair follicle development; positive regulation of translation; signal transduction

Disease: Pachyonychia Congenita 2; Steatocystoma Multiplex
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Research Articles on CK-17

1. Krt17 was demonstrated to contribute to areca nut-induced oral malignancy

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with CK-17 elisa kit

 Reference Product  PubMed Publications
 KRT6A elisa kit  >17 publications with CK-17 and KRT6A
 KRT7 elisa kit  >1 publications with CK-17 and KRT7

Pathways associated with CK-17 elisa kit

 Products by Pathway  Pathway Diagram
 EGFR1 Signaling Pathway antibodies  EGFR1 Signaling Pathway Diagram
 Glucocorticoid Receptor Regulatory Network Pathway antibodies  Glucocorticoid Receptor Regulatory Network Pathway Diagram

Diseases associated with CK-17 elisa kit

 Disease Name  Pubmed Publications
 Pachyonychia congenita Jackson Lawler type Antibodies  >20 publications with CK-17 and Pachyonychia congenita Jackson Lawler type
 Neoplasms Antibodies  >19 publications with CK-17 and Neoplasms
 Adenocarcinoma Antibodies  >7 publications with CK-17 and Adenocarcinoma
 Carcinoma, Squamous Cell Antibodies  >7 publications with CK-17 and Carcinoma, Squamous Cell
 Breast Neoplasms Antibodies  >5 publications with CK-17 and Breast Neoplasms
 Pain Antibodies  >4 publications with CK-17 and Pain
 Skin Neoplasms Antibodies  >3 publications with CK-17 and Skin Neoplasms
 Carcinoma, Basal Cell Antibodies  >2 publications with CK-17 and Carcinoma, Basal Cell
 Esophageal Squamous Cell Carcinoma Antibodies  >2 publications with CK-17 and Esophageal Squamous Cell Carcinoma
 Precancerous Conditions Antibodies  >1 publications with CK-17 and Precancerous Conditions

Organs/Tissues associated with CK-17 elisa kit

 Organ/Tissue Name  Pubmed Publications
 Skin Antibodies  >16 publications with CK-17 and Skin
 Mouth Antibodies  >3 publications with CK-17 and Mouth
 Lymph Antibodies  >2 publications with CK-17 and Lymph
 Mammary Gland Antibodies  >2 publications with CK-17 and Mammary Gland
 Lymph Node Antibodies  >2 publications with CK-17 and Lymph Node
 Intestine Antibodies  >1 publications with CK-17 and Intestine
 Bladder Antibodies  >1 publications with CK-17 and Bladder
 Prostate Antibodies  >1 publications with CK-17 and Prostate
 Muscle Antibodies  >1 publications with CK-17 and Muscle
 Pancreas Antibodies  >1 publications with CK-17 and Pancreas
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