Bloom Syndrome (BLM) ELISA Kit for Mouse

Name: Mouse BLM ELISA Kit; Bloom Syndrome; MyBioSource
Brand: MyBioSource
SKU: MBS284562
Price: 495 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS284562

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate BLM in samples. An antibody specific for BLM has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyBLM present is bound by the… More details

$495 / 48 Tests +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Request Current Manual

Product Overview

Online Datasheet in PDF

Product Name

Bloom syndrome (BLM), ELISA Kit

Full Product Name

Mouse Bloom syndrome protein (BLM) ELISA Kit

Product Gene Name

BLM elisa kit

Product Synonym Names

BS; RECQ2; RECQL2; RECQL3; RecQ Helicase-Like; DNA helicase, RecQ-like type 2

Species Reactivity

Mouse

Assay Type

Sandwich

Samples

Serum, Plasma, Other biological fluids

Specificity

This assay has high sensitivity and excellent specificity for detection of Mouse BLM. No significant cross-reactivity or interference between Mouse BLM and analogues was observed.

Preparation and Storage

Store at 2-8 degree C.

Molecular Weight

158,366 Da

3D Structure

ModBase 3D Structure for O88700

Sample Volume

1-200 uL

Precision

Intra-assay Precision (Precision within an assay)
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%) = SD/meanX100
Intra-Assay: CV
Inter-Assay: CV

Detection Wavelength

450 nm

ISO Certification

Manufactured in an ISO 9001:2015 Certified Laboratory.

Other Notes

Small volumes of BLM elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for BLM purchase

MBS284562 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Bloom syndrome (BLM) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing BLM. The ELISA analytical biochemical technique of the MBS284562 kit is based on BLM antibody-BLM antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect BLM antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, BLM. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for BLM. It may not necessarily be applicable to this product.

NCBI GI #

110225360

NCBI GeneID

12144

NCBI Accession #

NP_001035992.1

UniProt Primary Accession #

O88700

UniProt Secondary Accession #

O88198

UniProt Related Accession #

O88700

GenBank Nucleotide #

NM_001042527.2

NCBI Official Full Name

Bloom syndrome protein homolog isoform 2

NCBI Official Synonym Full Names

Bloom syndrome, RecQ like helicase

NCBI Official Symbol

Blm

UniProt Protein Name

Bloom syndrome protein homolog

UniProt Synonym Protein Names

RecQ helicase homolog

Protein Family

Bloom syndrome protein

UniProt Gene Name

Blm

UniProt Synonym Gene Names

mBLM

NCBI Protein Information

Bloom syndrome protein homolog

UniProt Comments for BLM

BLM: a magnesium-dependent ATP-dependent DNA-helicase that unwinds single- and double-stranded DNA in a 3'-5' direction. A member of the RecQ helicase family that is required for genome stability. Participates in DNA replication, recombination and repair. Part of the BRCA1-associated genome surveillance complex (BASC), which contains BRCA1, MSH2, MSH6, MLH1, ATM, BLM, PMS2 and the RAD50-MRE11-NBS1 protein complex which is a dynamic process changing throughout the cell cycle and within subnuclear domains. Interacts with ubiquitinated FANCD2.

Protein type: DNA repair, damage; DNA replication; EC 3.6.4.12; Helicase

Chromosomal Location of Human Ortholog: 7 D2|7 45.65 cM

Cellular Component: chromosome, telomeric region; cytoplasm; cytosol; intracellular; lateral element; male germ cell nucleus; nuclear chromosome; nuclear matrix; nucleolus; nucleus; PML body; pronucleus; replication fork

Molecular Function: ATP binding; ATP-dependent 3'-5' DNA helicase activity; ATP-dependent DNA helicase activity; ATP-dependent helicase activity; ATPase activity; bubble DNA binding; catalytic activity; DNA binding; DNA helicase activity; DNA-dependent ATPase activity; forked DNA-dependent helicase activity; four-way junction DNA binding; four-way junction helicase activity; G-quadruplex DNA binding; helicase activity; hydrolase activity; hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides; metal ion binding; nucleic acid binding; nucleotide binding; p53 binding; protein binding; protein homodimerization activity; single-stranded DNA binding; telomeric D-loop binding; Y-form DNA binding; zinc ion binding

Biological Process: alpha-beta T cell differentiation; cellular metabolic process; cellular response to DNA damage stimulus; cellular response to ionizing radiation; chromosome organization and biogenesis; DNA double-strand break processing; DNA duplex unwinding; DNA recombination; DNA repair; DNA replication; G-quadruplex DNA unwinding; mitotic cell cycle G2/M transition DNA damage checkpoint; negative regulation of cell division; negative regulation of DNA recombination; negative regulation of mitotic recombination; positive regulation of alpha-beta T cell proliferation; positive regulation of double-strand break repair via homologous recombination; positive regulation of transcription, DNA-templated; protein homooligomerization; protein oligomerization; regulation of binding; regulation of cyclin-dependent protein kinase activity; regulation of DNA-dependent DNA replication; replication fork processing; replication-born double-strand break repair via sister chromatid exchange; response to X-ray; telomere maintenance; telomeric D-loop disassembly

Research Articles on BLM

1. The anti-recombinase activity of BLM is of general importance for normal retention of RAD51 at DNA double strand break sites and regulation of homologous recombination.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with BLM elisa kit

Reference Product	PubMed Publications
Rad51 elisa kit	>67 publications with BLM and Rad51

Pathways associated with BLM elisa kit

Products by Pathway	Pathway Diagram
BRCA1-associated Genome Surveillance Complex (BASC) Pathway antibodies	BRCA1-associated Genome Surveillance Complex (BASC) Pathway Diagram
Bloom's Syndrome Complex Pathway antibodies	Bloom's Syndrome Complex Pathway Diagram
Fanconi Anemia Pathway antibodies	Fanconi Anemia Pathway Diagram
Fanconi Anemia Pathway antibodies	Fanconi Anemia Pathway Diagram
Homologous Recombination Pathway antibodies	Homologous Recombination Pathway Diagram
Homologous Recombination Pathway antibodies	Homologous Recombination Pathway Diagram

Diseases associated with BLM elisa kit

Disease Name	Pubmed Publications
Neoplasms Antibodies	>581 publications with BLM and Neoplasms
Bloom Syndrome Antibodies	>393 publications with BLM and Bloom Syndrome
Drug Toxicity Antibodies	>363 publications with BLM and Drug Toxicity
Disease Models, Animal Antibodies	>170 publications with BLM and Disease Models, Animal
Neoplasms, Experimental Antibodies	>139 publications with BLM and Neoplasms, Experimental
Head and Neck Neoplasms Antibodies	>131 publications with BLM and Head and Neck Neoplasms
Chromosome Aberrations Antibodies	>123 publications with BLM and Chromosome Aberrations
Carcinoma, Squamous Cell Antibodies	>114 publications with BLM and Carcinoma, Squamous Cell
Inflammation Antibodies	>90 publications with BLM and Inflammation
Skin Diseases Antibodies	>78 publications with BLM and Skin Diseases

Organs/Tissues associated with BLM elisa kit

Organ/Tissue Name	Pubmed Publications
Lung Antibodies	>376 publications with BLM and Lung
Intestine Antibodies	>91 publications with BLM and Intestine
Bone Antibodies	>58 publications with BLM and Bone
Brain Antibodies	>52 publications with BLM and Brain
Spleen Antibodies	>36 publications with BLM and Spleen
Bone Marrow Antibodies	>33 publications with BLM and Bone Marrow
Ovary Antibodies	>33 publications with BLM and Ovary
Thymus Antibodies	>25 publications with BLM and Thymus
Heart Antibodies	>25 publications with BLM and Heart
Stomach Antibodies	>19 publications with BLM and Stomach