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GRINL1A ELISA Kit for Rat

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS284628

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate GRINL1A in samples. An antibody specific for GRINL1A has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyGRINL1A present is bound by… More details

$495 / 48 Tests +1 FREE 8GB USB

Product Documentation

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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
GRINL1A, ELISA Kit
Full Product Name
Rat Protein GRINL1A, isoforms 4/5 (GRINL1A) ELISA Kit
Product Gene Name
GRINL1A elisa kit
Product Synonym Names
POLR2M; DNA-directed RNA polymerase II subunit M; Glutamate receptor-like protein 1A
Species Reactivity
Rat
Assay Type
Sandwich
Samples
Serum, Plasma, Other biological fluids
Specificity
This assay has high sensitivity and excellent specificity for detection of Rat GRINL1A. No significant cross-reactivity or interference between Rat GRINL1A and analogues was observed.
Preparation and Storage
Store at 2-8 degree C.
Molecular Weight
40,874 Da
3D Structure
ModBase 3D Structure for Q91XQ4
Sample Volume
1-200 uL
Precision
Intra-assay Precision (Precision within an assay)
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%) = SD/meanX100
Intra-Assay: CV
Inter-Assay: CV
Detection Wavelength
450 nm
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.

Other Notes

Small volumes of GRINL1A elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for GRINL1A purchase

MBS284628 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the GRINL1A, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing GRINL1A. The ELISA analytical biochemical technique of the MBS284628 kit is based on GRINL1A antibody-GRINL1A antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect GRINL1A antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, GRINL1A. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for GRINL1A. It may not necessarily be applicable to this product.

NCBI GI #
34447223
NCBI GeneID
192147
NCBI Accession #
NP_899652.1
UniProt Primary Accession #
Q91XQ4
UniProt Related Accession #
Q91XQ4
GenBank Nucleotide #
NM_183402.1
NCBI Official Full Name
DNA-directed RNA polymerase II subunit GRINL1A
NCBI Official Synonym Full Names
RNA polymerase II subunit M
NCBI Official Symbol
Polr2m
NCBI Official Synonym Symbols
Glurr; Grinl1a
UniProt Protein Name
DNA-directed RNA polymerase II subunit GRINL1A
UniProt Synonym Protein Names
DNA-directed RNA polymerase II subunit M; Glutamate receptor-like protein 1A
Protein Family
DNA-directed RNA polymerase
UniProt Gene Name
Polr2m
UniProt Synonym Gene Names
Grinl1a
NCBI Protein Information
DNA-directed RNA polymerase II subunit GRINL1A

NCBI Summary for GRINL1A

putative glutamate receptor that may have a role in susceptibility to autism [RGD, Feb 2006]

UniProt Comments for GRINL1A

GRINL1A: Isoform 1 appears to be a stable component of the Pol II(G) complex form of RNA polymerase II (Pol II). Pol II synthesizes mRNA precursors and many functional non-coding RNAs and is the central component of the basal RNA polymerase II transcription machinery. Isoform 1 may play a role in the Mediator complex-dependent regulation of transcription activation. Isoform 1 acts in vitro as a negative regulator of transcriptional activation; this repression is relieved by the Mediator complex, which restores Pol II(G) activator-dependent transcription to a level equivalent to that of Pol II. Belongs to the GRINL1 family. 6 isoforms of the human protein are produced by alternative splicing.

Protein type: Channel, ligand-gated; Nuclear envelope; Transferase

Chromosomal Location of Human Ortholog: 8q24

Cellular Component: cell soma; DNA-directed RNA polymerase II, holoenzyme; nuclear envelope

Molecular Function: DNA-directed RNA polymerase activity

Biological Process: maintenance of ER location; RNA biosynthetic process
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with GRINL1A elisa kit

 Reference Product  PubMed Publications
 Rpap2 elisa kit  >1 publications with GRINL1A and Rpap2
 Polr2e elisa kit  >1 publications with GRINL1A and Polr2e

Diseases associated with GRINL1A elisa kit

 Disease Name  Pubmed Publications
 Carcinoma Antibodies  >1 publications with GRINL1A and Carcinoma

Organs/Tissues associated with GRINL1A elisa kit

 Organ/Tissue Name  Pubmed Publications
 Heart Antibodies  >1 publications with GRINL1A and Heart
 Brain Antibodies  >1 publications with GRINL1A and Brain
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