Nitric Oxide Synthase (NOS2) ELISA Kit for Rabbit

Name: Rabbit NOS2 ELISA Kit; Nitric Oxide Synthase; MyBioSource
Brand: MyBioSource
SKU: MBS284669
Price: 545 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS284669

Intended Uses: For the quantitative detection of Rabbit Nitric oxide synthase, inducible (NOS2) concentration in serum, plasma and other biological fluids.Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate NOS2 in Rabbit serum,… More details

$545 / 48 Tests +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Request Current Manual

Product Overview

Online Datasheet in PDF

Product Name

Nitric oxide synthase (NOS2), ELISA Kit

Full Product Name

Rabbit Nitric oxide synthase, inducible (NOS2) ELISA Kit

Product Gene Name

NOS2 elisa kit

Product Synonym Names

HEP-NOS; INOS; NOS; NOS2A; NOS; type II|nitric oxide synthase 2A|nitric oxide synthase 2A (inducible; hepatocytes)|nitric oxide synthase; macrophage

Species Reactivity

Rabbit

Assay Type

Quantitative Sandwich

Samples

Serum, plasma and other biological fluids

Detection Range

0.156 ng/mL - 10 ng/mL

Sensitivity

0.1 ng/mL

Specificity

This assay has high sensitivity and excellent specificity for detection of Rabbit NOS2. No significant cross-reactivity or interference between Rabbit NOS2 and analogues was observed.

Preparation and Storage

Store at 2-8 degree C.

Inter-assay Precision

Inter-assay Precision (Precision between assays) Three samples of known concentration were tested in forty separate assays to assess inter-assay precision. CV (%) = SD/meanX100. Inter-Assay: CV<12%

Intra-assay Precision

Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision. Intra-Assay: CV<8%

Molecular Weight

67,876 Da

OMIM

163729

ISO Certification

Manufactured in an ISO 9001:2015 Certified Laboratory.

Other Notes

Small volumes of NOS2 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for NOS2 purchase

MBS284669 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Nitric oxide synthase (NOS2) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing NOS2. The ELISA analytical biochemical technique of the MBS284669 kit is based on NOS2 antibody-NOS2 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect NOS2 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, NOS2. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for NOS2. It may not necessarily be applicable to this product.

NCBI GI #

4262036

NCBI GeneID

4846

NCBI Accession #

AAD14336.1

UniProt Secondary Accession #

Q13662; Q14251; Q14434; Q548C1; Q6GSL5; Q9UDC6; A0S0A7; A0S0A8; A8KA63; B2RCQ1; E9PFR2

UniProt Related Accession #

P29474

NCBI Official Full Name

nitric oxide synthase, partial

NCBI Official Synonym Full Names

nitric oxide synthase 3

NCBI Official Symbol

NOS3

NCBI Official Synonym Symbols

eNOS; ECNOS

UniProt Protein Name

Nitric oxide synthase, endothelial

UniProt Synonym Protein Names

Constitutive NOS; cNOS; EC-NOS; Endothelial NOS; eNOS; NOS type III; NOSIII

Protein Family

Nitric oxide synthase

UniProt Gene Name

NOS3

UniProt Synonym Gene Names

cNOS; eNOS; NOSIII

UniProt Entry Name

NOS3_HUMAN

NCBI Protein Information

nitric oxide synthase, endothelial

NCBI Summary for NOS2

Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. Nitric oxide is synthesized from L-arginine by nitric oxide synthases. Variations in this gene are associated with susceptibility to coronary spasm. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2009]

UniProt Comments for NOS2

eNOS: endothelial constitutive nitric oxide synthase. Synthesizes nitric oxide (NO) from arginine and oxygen, which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway. NO mediates vascular endothelial growth factor (VEGF)-induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets. Induced in humans by caloric restriction. Stimulated by calcium/calmodulin. Inhibited by NOSIP and NOSTRIN.

Protein type: Oxidoreductase; EC 1.14.13.39; Amino Acid Metabolism - arginine and proline

Chromosomal Location of Human Ortholog: 7q36

Cellular Component: caveola; cytoplasm; cytoskeleton; cytosol; Golgi membrane; nucleus; plasma membrane

Molecular Function: actin monomer binding; cadmium ion binding; calmodulin binding; FAD binding; FMN binding; heme binding; iron ion binding; NADP binding; nitric-oxide synthase activity; protein binding

Biological Process: angiogenesis; arginine catabolic process; blood coagulation; blood vessel remodeling; cell redox homeostasis; endothelial cell migration; in utero embryonic development; lipopolysaccharide-mediated signaling pathway; lung development; mitochondrion organization and biogenesis; negative regulation of blood pressure; negative regulation of calcium ion transport; negative regulation of cell proliferation; negative regulation of hydrolase activity; negative regulation of muscle hyperplasia; negative regulation of potassium ion transport; nitric oxide biosynthetic process; nitric oxide mediated signal transduction; nitric oxide metabolic process; ovulation from ovarian follicle; positive regulation of angiogenesis; positive regulation of guanylate cyclase activity; positive regulation of vasodilation; regulation of blood pressure; regulation of blood vessel size; regulation of nitric-oxide synthase activity; regulation of sodium ion transport; regulation of systemic arterial blood pressure by endothelin; regulation of the force of heart contraction by chemical signal; removal of superoxide radicals; response to heat; smooth muscle hyperplasia; vascular endothelial growth factor receptor signaling pathway

Disease: Alzheimer Disease; Hypertension, Essential; Preeclampsia/eclampsia 1; Stroke, Ischemic

Research Articles on NOS2

1. eNOS intron 4 gene polymorphism is related to endothelial dysfunction and subclinical atherosclerosis and could be a possible genetic marker for prediction of increased susceptibility to cardiovascular complications.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with NOS2 elisa kit

Reference Product	PubMed Publications
JUN elisa kit	>580 publications with NOS2 and JUN
AKT1 elisa kit	>128 publications with NOS2 and AKT1
CAV1 elisa kit	>105 publications with NOS2 and CAV1
HIF1A elisa kit	>58 publications with NOS2 and HIF1A
ESR1 elisa kit	>11 publications with NOS2 and ESR1
NOSIP elisa kit	>6 publications with NOS2 and NOSIP
NOSTRIN elisa kit	>6 publications with NOS2 and NOSTRIN
HSP90AA1 elisa kit	>3 publications with NOS2 and HSP90AA1
KNG1 elisa kit	>2 publications with NOS2 and KNG1

Pathways associated with NOS2 elisa kit

Products by Pathway	Pathway Diagram
ACE Inhibitor Pathway antibodies	ACE Inhibitor Pathway Diagram
AGE-RAGE Signaling Pathway In Diabetic Complications antibodies	AGE-RAGE Signaling Pathway In Diabetic Complications Diagram
AGE-RAGE Signaling Pathway In Diabetic Complications antibodies	AGE-RAGE Signaling Pathway In Diabetic Complications Diagram
AGE/RAGE Pathway antibodies	AGE/RAGE Pathway Diagram
Angiogenesis Pathway antibodies	Angiogenesis Pathway Diagram
Angiopoietin Receptor Tie2-mediated Signaling Pathway antibodies	Angiopoietin Receptor Tie2-mediated Signaling Pathway Diagram
Arginine And Proline Metabolism Pathway antibodies	Arginine And Proline Metabolism Pathway Diagram
Arginine And Proline Metabolism Pathway antibodies	Arginine And Proline Metabolism Pathway Diagram
Arginine Biosynthesis Pathway antibodies	Arginine Biosynthesis Pathway Diagram
Arginine Biosynthesis Pathway antibodies	Arginine Biosynthesis Pathway Diagram

Diseases associated with NOS2 elisa kit

Disease Name	Pubmed Publications
Cardiovascular Diseases Antibodies	>2325 publications with NOS2 and Cardiovascular Diseases
Hypertension Antibodies	>1140 publications with NOS2 and Hypertension
Myocardial Ischemia Antibodies	>608 publications with NOS2 and Myocardial Ischemia
Atherosclerosis Antibodies	>489 publications with NOS2 and Atherosclerosis
Reperfusion Injury Antibodies	>313 publications with NOS2 and Reperfusion Injury
Myocardial Infarction Antibodies	>271 publications with NOS2 and Myocardial Infarction
Coronary Artery Disease Antibodies	>270 publications with NOS2 and Coronary Artery Disease
Diabetes Mellitus, Experimental Antibodies	>219 publications with NOS2 and Diabetes Mellitus, Experimental
Insulin Resistance Antibodies	>182 publications with NOS2 and Insulin Resistance
Heart Failure Antibodies	>178 publications with NOS2 and Heart Failure

Organs/Tissues associated with NOS2 elisa kit

Organ/Tissue Name	Pubmed Publications
Vascular Antibodies	>3867 publications with NOS2 and Vascular
Blood Antibodies	>2943 publications with NOS2 and Blood
Heart Antibodies	>1349 publications with NOS2 and Heart
Kidney Antibodies	>567 publications with NOS2 and Kidney
Brain Antibodies	>478 publications with NOS2 and Brain
Lung Antibodies	>422 publications with NOS2 and Lung
Liver Antibodies	>296 publications with NOS2 and Liver
Embryonic Tissue Antibodies	>218 publications with NOS2 and Embryonic Tissue
Bone Antibodies	>166 publications with NOS2 and Bone
Skin Antibodies	>107 publications with NOS2 and Skin