Cystic Fibrosis Transmembrane Conductance Regulator (CFTR) ELISA Kit for Rabbit

Name: Rabbit CFTR ELISA Kit; MyBioSource
Brand: MyBioSource
SKU: MBS284684
Price: 545 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS284684

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate CFTR in samples. An antibody specific for CFTR has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyCFTR present is bound by the… More details

$545 / 48 Tests +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Request Current Manual

Product Overview

Online Datasheet in PDF

Product Name

Cystic fibrosis transmembrane conductance regulator (CFTR), ELISA Kit

Full Product Name

Rabbit Cystic fibrosis transmembrane conductance regulator (CFTR) ELISA Kit

Product Gene Name

CFTR elisa kit

Product Synonym Names

TP-Binding Cassette Subfamily C,Member 7; Channel conductance-controlling ATPase; cAMP-dependent chloride channel

Species Reactivity

Rabbit

Assay Type

Sandwich

Samples

Serum, Plasma, Other biological fluids

Specificity

This assay has high sensitivity and excellent specificity for detection of Rabbit CFTR. No significant cross-reactivity or interference between Rabbit CFTR and analogues was observed.

Preparation and Storage

Store at 2-8 degree C.

Molecular Weight

164,701 Da

3D Structure

ModBase 3D Structure for Q00554

Sample Volume

1-200 uL

Precision

Intra-assay Precision (Precision within an assay)
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%) = SD/meanX100
Intra-Assay: CV
Inter-Assay: CV

Detection Wavelength

450 nm

ISO Certification

Manufactured in an ISO 9001:2015 Certified Laboratory.

Other Notes

Small volumes of CFTR elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CFTR purchase

MBS284684 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Cystic fibrosis transmembrane conductance regulator (CFTR) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing CFTR. The ELISA analytical biochemical technique of the MBS284684 kit is based on CFTR antibody-CFTR antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect CFTR antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, CFTR. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for CFTR. It may not necessarily be applicable to this product.

NCBI GI #

130501009

NCBI GeneID

100009471

NCBI Accession #

NP_001076185.1

UniProt Primary Accession #

Q00554

UniProt Secondary Accession #

Q09YM9; Q9TSD4; Q9TTX9; Q9TTY9

UniProt Related Accession #

Q00554

GenBank Nucleotide #

NM_001082716.1

NCBI Official Full Name

cystic fibrosis transmembrane conductance regulator

NCBI Official Symbol

CFTR

UniProt Protein Name

Cystic fibrosis transmembrane conductance regulator

UniProt Synonym Protein Names

ATP-binding cassette sub-family C member 7; Channel conductance-controlling ATPase (EC:3.6.3.49

Protein Family

Cystic fibrosis transmembrane conductance regulator

UniProt Gene Name

CFTR

UniProt Synonym Gene Names

ABCC7; CFTR

NCBI Protein Information

cystic fibrosis transmembrane conductance regulator

UniProt Comments for CFTR

Epithelial ion channel that plays an important role in the regulation of epithelial ion and water transport and fluid homeostasis. Mediates the transport of chloride ions across the cell membrane (). Channel activity is coupled to ATP hydrolysis. The ion channel is also permeable to HCO3-; selectivity depends on the extracellular chloride concentration. Exerts its function also by modulating the activity of other ion channels and transporters. Contributes to the regulation of the pH and the ion content of the epithelial fluid layer. Modulates the activity of the epithelial sodium channel (ENaC) complex, in part by regulating the cell surface expression of the ENaC complex. May regulate bicarbonate secretion and salvage in epithelial cells by regulating the transporter SLC4A7. Can inhibit the chloride channel activity of ANO1 (). Plays a role in the chloride and bicarbonate homeostasis during sperm epididymal maturation and capacitation ().

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Diseases associated with CFTR elisa kit

Disease Name	Pubmed Publications
Fibrosis Antibodies	>6846 publications with CFTR and Fibrosis
Cystic Fibrosis Antibodies	>6841 publications with CFTR and Cystic Fibrosis
Inflammation Antibodies	>428 publications with CFTR and Inflammation
Pancreatitis Antibodies	>291 publications with CFTR and Pancreatitis
Infertility, Male Antibodies	>255 publications with CFTR and Infertility, Male
Pancreatitis, Chronic Antibodies	>215 publications with CFTR and Pancreatitis, Chronic
Neoplasms Antibodies	>191 publications with CFTR and Neoplasms
BRONCHIECTASIS WITH OR WITHOUT ELEVATED SWEAT CHLORIDE 1 Antibodies	>155 publications with CFTR and BRONCHIECTASIS WITH OR WITHOUT ELEVATED SWEAT CHLORIDE 1
Liver Diseases Antibodies	>115 publications with CFTR and Liver Diseases
Azoospermia Antibodies	>98 publications with CFTR and Azoospermia