Neurotrophin Receptor-Interacting Factor Homolog (ZNF274) ELISA Kit for Bovine

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate ZNF274 in samples. An antibody specific for ZNF274 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyZNF274 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ZNF274 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ZNF274 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Small volumes of ZNF274 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS284722 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Neurotrophin receptor-interacting factor homolog (ZNF274) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing ZNF274. The ELISA analytical biochemical technique of the MBS284722 kit is based on ZNF274 antibody-ZNF274 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect ZNF274 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, ZNF274. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).