Tyrosine-Protein Kinase Yes (YES1) ELISA Kit for Canine

YES1 is the cellular homolog of the Yamaguchi sarcoma virus oncogene. The encoded protein has tyrosine kinase activity and belongs to the src family of proteins. This gene lies in close proximity to thymidylate synthase gene on chromosome 18, and a corresponding pseudogene has been found on chromosome 22.
The other 5 fragments could not be mapped either because hybridization signals were too weak or differentiation from mouse YES fragments was impossible. There was evidence for multiple copies of YES-related genes in the human genome, but only a single RNA species, 4.8 kb long, was found. At least 3 of the human YES gene copies had both introns and exons and 1 gene copy appeared to be a pseudogene.

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate YES1 in samples. An antibody specific for YES1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyYES1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for YES1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of YES1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Small volumes of YES1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS284726 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Tyrosine-protein kinase Yes (YES1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing YES1. The ELISA analytical biochemical technique of the MBS284726 kit is based on YES1 antibody-YES1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect YES1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, YES1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).