Interleukin-1 (IRAK-4) ELISA Kit for Human

Name: Human IRAK-4 ELISA Kit; Interleukin-1; MyBioSource
Brand: MyBioSource
SKU: MBS284732
Price: 495 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS284732

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate IRAK4 in samples. An antibody specific for IRAK4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyIRAK4 present is bound by the… More details

$495 / 48 Tests +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Request Current Manual

Product Overview

Online Datasheet in PDF

Product Name

Interleukin-1 (IRAK-4), ELISA Kit

Full Product Name

Human Interleukin-1 receptor-associated kinase 4 (IRAK-4) ELISA Kit

Product Gene Name

IRAK-4 elisa kit

Product Synonym Names

IPD1; NY-REN-64; REN64;

Species Reactivity

Human

Assay Type

Sandwich

Samples

Serum, Plasma, Other biological fluids

Specificity

This assay has high sensitivity and excellent specificity for detection of Human IRAK4. No significant cross-reactivity or interference between Human IRAK4 and analogues was observed.

Preparation and Storage

Store at 2-8 degree C.

Molecular Weight

37,674 Da

OMIM

AF445802 mRNA

3D Structure

ModBase 3D Structure for Q9NWZ3

Sample Volume

1-200 uL

Precision

Intra-assay Precision (Precision within an assay)
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%) = SD/meanX100
Intra-Assay: CV
Inter-Assay: CV

Detection Wavelength

450 nm

ISO Certification

Manufactured in an ISO 9001:2015 Certified Laboratory.

Other Notes

Small volumes of IRAK-4 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for IRAK-4 purchase

MBS284732 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Interleukin-1 (IRAK-4) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing IRAK-4. The ELISA analytical biochemical technique of the MBS284732 kit is based on IRAK-4 antibody-IRAK-4 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect IRAK-4 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, IRAK-4. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for IRAK-4. It may not necessarily be applicable to this product.

NCBI GI #

166795295

NCBI GeneID

51135

NCBI Accession #

NP_001107654.1

UniProt Primary Accession #

Q9NWZ3

UniProt Secondary Accession #

Q69FE1; Q8TDF7; Q9Y589

UniProt Related Accession #

Q9NWZ3

GenBank Nucleotide #

NM_001114182.2

NCBI Official Full Name

interleukin-1 receptor-associated kinase 4 isoform a

NCBI Official Synonym Full Names

interleukin 1 receptor associated kinase 4

NCBI Official Symbol

IRAK4

NCBI Official Synonym Symbols

IPD1; REN64; IRAK-4; NY-REN-64

UniProt Protein Name

Interleukin-1 receptor-associated kinase 4

UniProt Synonym Protein Names

Renal carcinoma antigen NY-REN-64

Protein Family

Interleukin-1-binding protein

UniProt Gene Name

IRAK4

UniProt Synonym Gene Names

IRAK-4

NCBI Protein Information

interleukin-1 receptor-associated kinase 4

NCBI Summary for IRAK-4

This gene encodes a kinase that activates NF-kappaB in both the Toll-like receptor (TLR) and T-cell receptor (TCR) signaling pathways. The protein is essential for most innate immune responses. Mutations in this gene result in IRAK4 deficiency and recurrent invasive pneumococcal disease. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2011]

UniProt Comments for IRAK-4

IRAK4: a TKL kinase of the IRAK family. The interleukin-1 receptor-associated kinases are important mediators in the signal transduction of Toll-like receptor and IL1R family members, collectively referred to as TIRs.

Protein type: EC 2.7.11.1; IRAK family; Kinase, protein; Protein kinase, Ser/Thr (non-receptor); Protein kinase, TKL; TKL group

Chromosomal Location of Human Ortholog: 12q12

Cellular Component: cytoplasm; cytosol; endosome membrane; extracellular space; nucleus; plasma membrane

Molecular Function: ATP binding; interleukin-1 receptor binding; magnesium ion binding; protein binding; protein kinase activity; protein serine/threonine kinase activity

Biological Process: cytokine production; innate immune response; JNK cascade; MyD88-dependent toll-like receptor signaling pathway; neutrophil mediated immunity; neutrophil migration; positive regulation of I-kappaB kinase/NF-kappaB signaling; positive regulation of NF-kappaB transcription factor activity; positive regulation of smooth muscle cell proliferation; toll-like receptor 9 signaling pathway; toll-like receptor signaling pathway

Disease: Invasive Pneumococcal Disease, Recurrent Isolated, 1; Irak4 Deficiency

Research Articles on IRAK-4

1. Data suggest that IRAK4 activity regulates activation of IRF5, TAK1, and IKKB in monocytes; IRAK4 activation of TAK1-IKKB-IRF5 axis leads to induction of cytokines and interferons following TLR7/TLR8 stimulation. (IRAK4 = interleukin-1 receptor-associated kinase 4; IRF5 = interferon regulatory factor-5; TAK1 = MAPK kinase kinase 7; IKKB = I-kappa B kinase; TLR = toll-like receptor)

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with IRAK-4 elisa kit

Reference Product	PubMed Publications
MYD88 elisa kit	>58 publications with IRAK-4 and MYD88
IRAK1 elisa kit	>37 publications with IRAK-4 and IRAK1
TRAF6 elisa kit	>26 publications with IRAK-4 and TRAF6
PELI1 elisa kit	>4 publications with IRAK-4 and PELI1
PELI2 elisa kit	>1 publications with IRAK-4 and PELI2

Pathways associated with IRAK-4 elisa kit

Products by Pathway	Pathway Diagram
Activated TLR4 Signalling Pathway antibodies	Activated TLR4 Signalling Pathway Diagram
Apoptosis Pathway antibodies	Apoptosis Pathway Diagram
Apoptosis Pathway antibodies	Apoptosis Pathway Diagram
Chagas Disease (American Trypanosomiasis) Pathway antibodies	Chagas Disease (American Trypanosomiasis) Pathway Diagram
Chagas Disease (American Trypanosomiasis) Pathway antibodies	Chagas Disease (American Trypanosomiasis) Pathway Diagram
Cytokine Signaling In Immune System Pathway antibodies	Cytokine Signaling In Immune System Pathway Diagram
IL-1 Signaling Pathway antibodies	IL-1 Signaling Pathway Diagram
IL1-mediated Signaling Events Pathway antibodies	IL1-mediated Signaling Events Pathway Diagram
Immune System Pathway antibodies	Immune System Pathway Diagram
Influenza A Pathway antibodies	Influenza A Pathway Diagram

Diseases associated with IRAK-4 elisa kit

Disease Name	Pubmed Publications
IRAK4 DEFICIENCY Antibodies	>42 publications with IRAK-4 and IRAK4 DEFICIENCY
Inflammation Antibodies	>30 publications with IRAK-4 and Inflammation
Necrosis Antibodies	>25 publications with IRAK-4 and Necrosis
Death Antibodies	>21 publications with IRAK-4 and Death
Lymphoma, B-Cell Antibodies	>3 publications with IRAK-4 and Lymphoma, B-Cell
Breast Neoplasms Antibodies	>1 publications with IRAK-4 and Breast Neoplasms
Cell Transformation, Neoplastic Antibodies	>1 publications with IRAK-4 and Cell Transformation, Neoplastic
Encephalomyelitis, Autoimmune, Experimental Antibodies	>1 publications with IRAK-4 and Encephalomyelitis, Autoimmune, Experimental
Ovarian Neoplasms Antibodies	>1 publications with IRAK-4 and Ovarian Neoplasms
Prostatic Neoplasms Antibodies	>1 publications with IRAK-4 and Prostatic Neoplasms

Organs/Tissues associated with IRAK-4 elisa kit

Organ/Tissue Name	Pubmed Publications
Blood Antibodies	>45 publications with IRAK-4 and Blood
Bone Antibodies	>10 publications with IRAK-4 and Bone
Bone Marrow Antibodies	>9 publications with IRAK-4 and Bone Marrow
Vascular Antibodies	>6 publications with IRAK-4 and Vascular
Lung Antibodies	>6 publications with IRAK-4 and Lung
Muscle Antibodies	>5 publications with IRAK-4 and Muscle
Brain Antibodies	>4 publications with IRAK-4 and Brain
Liver Antibodies	>4 publications with IRAK-4 and Liver
Skin Antibodies	>4 publications with IRAK-4 and Skin
Spleen Antibodies	>3 publications with IRAK-4 and Spleen