Af-10 (MLLT10) ELISA Kit for Human

Name: Human MLLT10 ELISA Kit; Af-10; MyBioSource
Brand: MyBioSource
SKU: MBS284779
Price: 495 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS284779

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate MLLT10 in Human serum, plasma. An antibody specific for MLLT10 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MLLT10 present… More details

$495 / 48 Tests +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Request Current Manual

Product Overview

Online Datasheet in PDF

Product Name

AF-10 (MLLT10), ELISA Kit

Full Product Name

Human Protein AF-10 (MLLT10) ELISA Kit

Product Gene Name

MLLT10 elisa kit

Product Synonym Names

RP11-399C16.2; AF10; DKFZp686E10210; MGC75086; ALL-1 fused gene from chromosome 10|myeloid/lymphoid or mixed-lineage leukemia (trithorax homolog); translocated to; 10|myeloid/lymphoid or mixed-linea

Species Reactivity

Human

Assay Type

Quantitative Sandwich

Samples

Serum, plasma and other biological fluids

Detection Range

0.31 ng/mL - 20 ng/mL

Sensitivity

0.15 ng/mL (mean of 6 independent assays)

Specificity

This assay has high sensitivity and excellent specificity for detection of Human MLLT10. No significant cross-reactivity or interference between Human MLLT10 and analogues was observed.

Preparation and Storage

Store at 2-8 degree C.

Inter-assay Precision

Inter-assay Precision (Precision between assays) Three samples of known concentration were tested in forty separate assays to assess inter-assay precision. CV (%) = SD/meanX100. Inter-Assay: CV<12%

Intra-assay Precision

Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision. Intra-Assay: CV<10%

Molecular Weight

20,047 Da

OMIM

U13948 mRNA

3D Structure

ModBase 3D Structure for P55197

ISO Certification

Manufactured in an ISO 9001:2015 Certified Laboratory.

Other Notes

Small volumes of MLLT10 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for MLLT10 purchase

MBS284779 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the AF-10 (MLLT10) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing MLLT10. The ELISA analytical biochemical technique of the MBS284779 kit is based on MLLT10 antibody-MLLT10 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect MLLT10 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, MLLT10. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for MLLT10. It may not necessarily be applicable to this product.

NCBI GI #

307548832

NCBI GeneID

8028

NCBI Accession #

NP_001182555.1

UniProt Primary Accession #

P55197

UniProt Secondary Accession #

Q5JT37; Q5VX90; Q66K63; B1ANA8

UniProt Related Accession #

P55197

GenBank Nucleotide #

NM_001195626.1

NCBI Official Full Name

protein AF-10 isoform c

NCBI Official Synonym Full Names

MLLT10, histone lysine methyltransferase DOT1L cofactor

NCBI Official Symbol

MLLT10

NCBI Official Synonym Symbols

AF10

UniProt Protein Name

Protein AF-10

UniProt Synonym Protein Names

ALL1-fused gene from chromosome 10 protein

Protein Family

Protein

UniProt Gene Name

MLLT10

UniProt Synonym Gene Names

AF10

NCBI Protein Information

protein AF-10

NCBI Summary for MLLT10

This gene encodes a transcription factor and has been identified as a partner gene involved in several chromosomal rearrangements resulting in various leukemias. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2010]

UniProt Comments for MLLT10

MLLT10: Probably involved in transcriptional regulation. In vitro or as fusion protein with MLL has transactivation activity. Binds to cruciform DNA. A chromosomal aberration involving MLLT10 is associated with acute leukemias. Translocation t(10;11)(p12;q23) with MLL/HRX. The result is a rogue activator protein. A chromosomal aberration involving MLLT10 is associated with diffuse histiocytic lymphomas. Translocation t(10;11)(p13;q14) with PICALM. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Oncoprotein; Transcription factor

Chromosomal Location of Human Ortholog: 10p12.31

Cellular Component: cytosol; nucleoplasm; nucleus; protein complex

Molecular Function: DNA binding; DNA binding transcription factor activity; metal ion binding; protein binding

Biological Process: positive regulation of transcription from RNA polymerase II promoter; transcription, DNA-dependent

Disease: Leukemia, Acute Myeloid

Research Articles on MLLT10

1. In a retroviral transduction/transplantation mouse model, mice transplanted with MLL/AF10(OM-LZ) cells harboring PTPN11(wt) developed myelomonocytic leukemia. Those transplanted with cells harboring PTPN11(G503A) -induced monocytic leukemia in a shorter latency. Adding PTPN11(G503A) to MLL/AF10 affected cell proliferation, chemo-resistance, differentiation, in vivo BM recruitment/clonal expansion and faster progression.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with MLLT10 elisa kit

Reference Product	PubMed Publications
MLLT3 elisa kit	>7 publications with MLLT10 and MLLT3
DOT1L elisa kit	>7 publications with MLLT10 and DOT1L
MLLT1 elisa kit	>5 publications with MLLT10 and MLLT1
YEATS4 elisa kit	>2 publications with MLLT10 and YEATS4

Diseases associated with MLLT10 elisa kit

Disease Name	Pubmed Publications
Leukemia, Myeloid, Acute Antibodies	>50 publications with MLLT10 and Leukemia, Myeloid, Acute
Central Nervous System Diseases Antibodies	>2 publications with MLLT10 and Central Nervous System Diseases
Meningioma Antibodies	>2 publications with MLLT10 and Meningioma
Recurrence Antibodies	>1 publications with MLLT10 and Recurrence
Brain Diseases Antibodies	>1 publications with MLLT10 and Brain Diseases
Neoplasm Metastasis Antibodies	>1 publications with MLLT10 and Neoplasm Metastasis

Organs/Tissues associated with MLLT10 elisa kit

Organ/Tissue Name	Pubmed Publications
Bone Antibodies	>18 publications with MLLT10 and Bone
Blood Antibodies	>16 publications with MLLT10 and Blood
Nerve Antibodies	>5 publications with MLLT10 and Nerve
Testis Antibodies	>5 publications with MLLT10 and Testis
Muscle Antibodies	>3 publications with MLLT10 and Muscle
Brain Antibodies	>3 publications with MLLT10 and Brain
Prostate Antibodies	>1 publications with MLLT10 and Prostate
Thymus Antibodies	>1 publications with MLLT10 and Thymus
Eye Antibodies	>1 publications with MLLT10 and Eye
Placenta Antibodies	>1 publications with MLLT10 and Placenta