Urocortin (UCN) ELISA Kit for Mouse

Name: Mouse UCN ELISA Kit; Urocortin; MyBioSource
Brand: MyBioSource
SKU: MBS284784
Price: 495 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS284784

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate UCN in samples. An antibody specific for UCN has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyUCN present is bound by the… More details

$495 / 48 Tests +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

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Product Overview

Online Datasheet in PDF

Product Name

Urocortin (UCN), ELISA Kit

Full Product Name

Mouse Urocortin (UCN) ELISA Kit

Product Gene Name

UCN elisa kit

Product Synonym Names

MGC129974; MGC129975; UI; UROC; urocortin; preproprotein

Species Reactivity

Mouse

Assay Type

Sandwich

Samples

Serum, Plasma, Other biological fluids

Specificity

This assay has high sensitivity and excellent specificity for detection of Mouse UCN. No significant cross-reactivity or interference between Mouse UCN and analogues was observed.

Preparation and Storage

Store at 2-8 degree C.

Molecular Weight

13,558 Da

3D Structure

ModBase 3D Structure for P81615

Sample Volume

1-200 uL

Precision

Intra-assay Precision (Precision within an assay)
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%) = SD/meanX100
Intra-Assay: CV
Inter-Assay: CV

Detection Wavelength

450 nm

ISO Certification

Manufactured in an ISO 9001:2015 Certified Laboratory.

Other Notes

Small volumes of UCN elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for UCN purchase

MBS284784 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Urocortin (UCN) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing UCN. The ELISA analytical biochemical technique of the MBS284784 kit is based on UCN antibody-UCN antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect UCN antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, UCN. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for UCN. It may not necessarily be applicable to this product.

NCBI GI #

1068937250

NCBI GeneID

22226

NCBI Accession #

NP_001332939.1

UniProt Primary Accession #

P81615

UniProt Secondary Accession #

O88390; Q14A76

UniProt Related Accession #

P81615

GenBank Nucleotide #

NM_001346010.1

NCBI Official Full Name

urocortin preproprotein

NCBI Official Synonym Full Names

urocortin

NCBI Official Symbol

Ucn

NCBI Official Synonym Symbols

Ucn1; Mpv17

UniProt Protein Name

Urocortin

Protein Family

Urocortin

UniProt Gene Name

Ucn

NCBI Protein Information

urocortin

NCBI Summary for UCN

This gene encodes a member of the corticotropin-releasing hormone peptide family that participates in coordinating autonomic, endocrine, and behavioral responses to stress. The encoded preproprotein undergoes proteolytic processing to generate a mature, functional hormone. Mice lacking the encoded protein exhibit a heightened anxiety-like behaviors and a significantly reduced acoustic startle response. [provided by RefSeq, Sep 2016]

UniProt Comments for UCN

UCN: Acts in vitro to stimulate the secretion of adrenocorticotropic hormone (ACTH). Binds with high affinity to CRF receptor types 1, 2-alpha, and 2-beta. Belongs to the sauvagine/corticotropin-releasing factor/urotensin I family.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 5 B1|5 17.11 cM

Cellular Component: axon; axon terminus; cell soma; dendrite; extracellular region; perikaryon

Molecular Function: corticotropin-releasing hormone receptor 1 binding; corticotropin-releasing hormone receptor 2 binding; G-protein coupled receptor binding; histone deacetylase inhibitor activity; hormone activity

Biological Process: activation of protein kinase A activity; aerobic respiration; associative learning; drinking behavior; feeding behavior; learning and/or memory; negative regulation of apoptosis; negative regulation of appetite; negative regulation of blood pressure; negative regulation of cell size; negative regulation of hormone secretion; neuron projection development; neuropeptide signaling pathway; pancreatic juice secretion; positive regulation of cAMP biosynthetic process; positive regulation of cell growth; positive regulation of collagen biosynthetic process; positive regulation of corticotropin secretion; positive regulation of DNA replication; positive regulation of interleukin-6 production; positive regulation of peptidyl-serine phosphorylation; positive regulation of protein phosphorylation; positive regulation of transcription from RNA polymerase II promoter; positive regulation of translation; positive regulation of vascular permeability; prepulse inhibition; regulation of synaptic transmission, glutamatergic; response to oxidative stress; response to starvation; sensory perception of sound; social behavior; startle response

Research Articles on UCN

1. Genetic deletion of Ucn1 reduced high levels of intake and preference across 2 weeks of continuous access drinking when alcohol concentrations were increased from 10 to 20 to 40%. Ucn1 deletion did not alter low-level drinking when the alcohol concentration remained at 10%.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with UCN elisa kit

Reference Product	PubMed Publications
Ucn2 elisa kit	>57 publications with UCN and Ucn2
Ucn3 elisa kit	>47 publications with UCN and Ucn3
Crhr2 elisa kit	>25 publications with UCN and Crhr2
Fos elisa kit	>23 publications with UCN and Fos
Crhr1 elisa kit	>21 publications with UCN and Crhr1
Crhbp elisa kit	>8 publications with UCN and Crhbp
Nps elisa kit	>3 publications with UCN and Nps

Pathways associated with UCN elisa kit

Products by Pathway	Pathway Diagram
Class B/2 (Secretin Family Receptors) Pathway antibodies	Class B/2 (Secretin Family Receptors) Pathway Diagram
GPCR Ligand Binding Pathway antibodies	GPCR Ligand Binding Pathway Diagram
Signal Transduction Pathway antibodies	Signal Transduction Pathway Diagram
Signaling By GPCR Pathway antibodies	Signaling By GPCR Pathway Diagram

Diseases associated with UCN elisa kit

Disease Name	Pubmed Publications
Death Antibodies	>105 publications with UCN and Death
Inflammation Antibodies	>42 publications with UCN and Inflammation
Heart Diseases Antibodies	>40 publications with UCN and Heart Diseases
Breast Neoplasms Antibodies	>39 publications with UCN and Breast Neoplasms
Lung Diseases Antibodies	>31 publications with UCN and Lung Diseases
Necrosis Antibodies	>25 publications with UCN and Necrosis
Congenital, Hereditary, and Neonatal Diseases and Abnormalities Antibodies	>22 publications with UCN and Congenital, Hereditary, and Neonatal Diseases and Abnormalities
Pain Antibodies	>21 publications with UCN and Pain
Neoplasms, Experimental Antibodies	>20 publications with UCN and Neoplasms, Experimental
Hypertension Antibodies	>17 publications with UCN and Hypertension