Acetylcholine (CHRNB1) ELISA Kit for Mouse

Name: Mouse CHRNB1 ELISA Kit; Acetylcholine; MyBioSource
Brand: MyBioSource
SKU: MBS284906
Price: 495 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS284906

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate CHRNB1 in samples. An antibody specific for CHRNB1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyCHRNB1 present is bound by… More details

$495 / 48 Tests +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Request Current Manual

Product Overview

Online Datasheet in PDF

Product Name

Acetylcholine (CHRNB1), ELISA Kit

Full Product Name

Mouse Acetylcholine receptor subunit beta (CHRNB1) ELISA Kit

Product Gene Name

CHRNB1 elisa kit

Product Synonym Names

N-AChRB1; NAChRB1; CMS2A; SCCMS; ACHRB; CHRNB; CMS1D; N-AChR-B1; Neuronal Acetylcholine Receptor Beta 1; Acetylcholine receptor subunit beta

Species Reactivity

Mouse

Assay Type

Sandwich

Samples

Serum, Plasma, Other biological fluids

Specificity

This assay has high sensitivity and excellent specificity for detection of Mouse CHRNB1. No significant cross-reactivity or interference between Mouse CHRNB1 and analogues was observed.

Preparation and Storage

Store at 2-8 degree C.

Molecular Weight

56,931 Da

3D Structure

ModBase 3D Structure for P09690

Sample Volume

1-200 uL

Precision

Intra-assay Precision (Precision within an assay)
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%) = SD/meanX100
Intra-Assay: CV
Inter-Assay: CV

Detection Wavelength

450 nm

ISO Certification

Manufactured in an ISO 9001:2015 Certified Laboratory.

Other Notes

Small volumes of CHRNB1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CHRNB1 purchase

MBS284906 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Acetylcholine (CHRNB1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing CHRNB1. The ELISA analytical biochemical technique of the MBS284906 kit is based on CHRNB1 antibody-CHRNB1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect CHRNB1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, CHRNB1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for CHRNB1. It may not necessarily be applicable to this product.

NCBI GI #

160358782

NCBI GeneID

11443

NCBI Accession #

NP_033731.3

UniProt Primary Accession #

P09690

UniProt Secondary Accession #

Q5F292

UniProt Related Accession #

P09690

GenBank Nucleotide #

NM_009601.4

NCBI Official Full Name

acetylcholine receptor subunit beta

NCBI Official Synonym Full Names

cholinergic receptor, nicotinic, beta polypeptide 1 (muscle)

NCBI Official Symbol

Chrnb1

NCBI Official Synonym Symbols

Acrb; Achr-2

UniProt Protein Name

Acetylcholine receptor subunit beta

Protein Family

Acetylcholine receptor

UniProt Gene Name

Chrnb1

UniProt Synonym Gene Names

Acrb

NCBI Protein Information

acetylcholine receptor subunit beta

UniProt Comments for CHRNB1

nAChRB1: After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane. Defects in CHRNB1 are a cause of congenital myasthenic syndrome slow-channel type (SCCMS). SCCMS is the most common congenital myasthenic syndrome. Congenital myasthenic syndromes are characterized by muscle weakness affecting the axial and limb muscles (with hypotonia in early-onset forms), the ocular muscles (leading to ptosis and ophthalmoplegia), and the facial and bulbar musculature (affecting sucking and swallowing, and leading to dysphonia). The symptoms fluctuate and worsen with physical effort. SCCMS is caused by kinetic abnormalities of the AChR, resulting in prolonged endplate currents and prolonged AChR channel opening episodes. Defects in CHRNB1 are a cause of congenital myasthenic syndrome with acetylcholine receptor deficiency (CMS-ACHRD). CMS-ACHRD is a postsynaptic congenital myasthenic syndrome. Mutations underlying AChR deficiency cause a 'loss of function' and show recessive inheritance. Belongs to the ligand-gated ion channel (TC 1.A.9) family. Acetylcholine receptor (TC 1.A.9.1) subfamily. Beta- 1/CHRNB1 sub-subfamily.

Protein type: Channel, cation; Channel, ligand-gated; Membrane protein, integral; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 11 B3|11 42.87 cM

Cellular Component: acetylcholine-gated channel complex; cell junction; integral component of membrane; membrane; plasma membrane; postsynaptic membrane; synapse

Molecular Function: acetylcholine binding; acetylcholine receptor activity; acetylcholine-gated cation channel activity; channel activity; extracellular ligand-gated ion channel activity; ion channel activity; ligand-gated ion channel activity; protein binding

Biological Process: behavioral response to nicotine; cation transport; ion transport; muscle contraction; muscle fiber development; neurological system process; neuromuscular synaptic transmission; postsynaptic membrane organization; regulation of membrane potential; response to nicotine; signal transduction; skeletal muscle contraction; synaptic transmission, cholinergic; transport

Research Articles on CHRNB1

1. Conformational changes involved in channel gating propagate from the binding-site to the channel in the ACh-binding subunits and subsequently spread to the non-binding subunit.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with CHRNB1 elisa kit

Products by Pathway	Pathway Diagram
Neuroactive Ligand-receptor Interaction Pathway antibodies	Neuroactive Ligand-receptor Interaction Pathway Diagram
Neuroactive Ligand-receptor Interaction Pathway antibodies	Neuroactive Ligand-receptor Interaction Pathway Diagram

Diseases associated with CHRNB1 elisa kit

Disease Name	Pubmed Publications
Substance-Related Disorders Antibodies	>5 publications with CHRNB1 and Substance-Related Disorders
Neoplasms Antibodies	>3 publications with CHRNB1 and Neoplasms
Carcinoma Antibodies	>3 publications with CHRNB1 and Carcinoma
Congenital Abnormalities Antibodies	>3 publications with CHRNB1 and Congenital Abnormalities
Hypokinesia Antibodies	>1 publications with CHRNB1 and Hypokinesia
Carcinoma, Squamous Cell Antibodies	>1 publications with CHRNB1 and Carcinoma, Squamous Cell
Hyperplasia Antibodies	>1 publications with CHRNB1 and Hyperplasia
Lung Neoplasms Antibodies	>1 publications with CHRNB1 and Lung Neoplasms

Organs/Tissues associated with CHRNB1 elisa kit

Organ/Tissue Name	Pubmed Publications
Joint Antibodies	>2 publications with CHRNB1 and Joint
Lung Antibodies	>2 publications with CHRNB1 and Lung