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Alpha-2-Antiplasmin (SERPINF2) ELISA Kit for Mouse

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS284982

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate SERPINF2 in samples. An antibody specific for SERPINF2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySERPINF2 present is bound… More details

$545 / 48 Tests +1 FREE 8GB USB

Product Documentation

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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
alpha-2-Antiplasmin (SERPINF2), ELISA Kit
Full Product Name
Mouse alpha-2-Antiplasmin (alpha-2-AP) ELISA Kit
Product Gene Name
SERPINF2 elisa kit
Product Synonym Names
alpha-2-AP; A2AP; AAP; ALPHA-2-PI; API; PLI; OTTHUMP00000115656|alpha-2-antiplasmin|alpha-2-plasmin inhibitor|serine (or cysteine) proteinase inhibitor; clade F (alpha-2 antiplasmin; pigment epithelium derived
Species Reactivity
Mouse
Assay Type
Sandwich
Samples
Serum, Plasma, Other biological fluids
Specificity
This assay has high sensitivity and excellent specificity for detection of Mouse SERPINF2. No significant cross-reactivity or interference between Mouse SERPINF2 and analogues was observed.
Preparation and Storage
Store at 2-8 degree C.
Molecular Weight
54,972 Da
3D Structure
ModBase 3D Structure for Q61247
Sample Volume
1-200 uL
Precision
Intra-assay Precision (Precision within an assay)
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%) = SD/meanX100
Intra-Assay: CV
Inter-Assay: CV
Detection Wavelength
450 nm
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.

Other Notes

Small volumes of SERPINF2 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for SERPINF2 purchase

MBS284982 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the alpha-2-Antiplasmin (SERPINF2) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing SERPINF2. The ELISA analytical biochemical technique of the MBS284982 kit is based on SERPINF2 antibody-SERPINF2 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect SERPINF2 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, SERPINF2. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for SERPINF2. It may not necessarily be applicable to this product.

NCBI GI #
6679383
NCBI GeneID
18816
NCBI Accession #
NP_032904.1
UniProt Primary Accession #
Q61247
UniProt Related Accession #
Q61247
GenBank Nucleotide #
NM_008878.2
NCBI Official Full Name
alpha-2-antiplasmin
NCBI Official Synonym Full Names
serine (or cysteine) peptidase inhibitor, clade F, member 2
NCBI Official Symbol
Serpinf2
NCBI Official Synonym Symbols
Pli; AI747498; Serpimf2
UniProt Protein Name
Alpha-2-antiplasmin
UniProt Synonym Protein Names
Alpha-2-plasmin inhibitor; Alpha-2-PI; Serpin F2
Protein Family
Alpha-2-antiplasmin
UniProt Gene Name
Serpinf2
UniProt Synonym Gene Names
Pli; Alpha-2-AP; Alpha-2-PI
NCBI Protein Information
alpha-2-antiplasmin

UniProt Comments for SERPINF2

SERPINF2: Serine protease inhibitor. The major targets of this inhibitor are plasmin and trypsin, but it also inactivates matriptase-3/TMPRSS7 and chymotrypsin. Defects in SERPINF2 are the cause of alpha-2-plasmin inhibitor deficiency (APLID). APLID is an autosomal recessive disorder resulting in severe hemorrhagic diathesis. Belongs to the serpin family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 11|11 B5

Cellular Component: cell surface; extracellular region; extracellular space; fibrinogen complex

Molecular Function: peptidase inhibitor activity; protease binding; protein homodimerization activity; serine-type endopeptidase inhibitor activity

Biological Process: acute-phase response; blood vessel morphogenesis; collagen fibril organization; negative regulation of fibrinolysis; negative regulation of peptidase activity; positive regulation of cell differentiation; positive regulation of collagen biosynthetic process; positive regulation of JNK cascade; positive regulation of smooth muscle cell proliferation; positive regulation of stress fiber formation; positive regulation of transcription from RNA polymerase II promoter; regulation of blood vessel diameter by renin-angiotensin

Disease: Alpha-2-plasmin Inhibitor Deficiency
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Research Articles on SERPINF2

1. alpha2AP has a profibrotic effect probably not by the action as a plasmin inhibitor, and the blocking of alpha2AP exerts an antifibrotic effect in humans and mice with systemic sclerosis

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with SERPINF2 elisa kit

 Products by Pathway  Pathway Diagram
 Blood Clotting Cascade Pathway antibodies  Blood Clotting Cascade Pathway Diagram
 Complement And Coagulation Cascades Pathway antibodies  Complement And Coagulation Cascades Pathway Diagram
 Complement And Coagulation Cascades Pathway antibodies  Complement And Coagulation Cascades Pathway Diagram
 Complement And Coagulation Cascades Pathway antibodies  Complement And Coagulation Cascades Pathway Diagram
 Dissolution Of Fibrin Clot Pathway antibodies  Dissolution Of Fibrin Clot Pathway Diagram
 Hemostasis Pathway antibodies  Hemostasis Pathway Diagram
 Platelet Activation, Signaling And Aggregation Pathway antibodies  Platelet Activation, Signaling And Aggregation Pathway Diagram
 Platelet Degranulation Pathway antibodies  Platelet Degranulation Pathway Diagram
 Response To Elevated Platelet Cytosolic Ca2+ Pathway antibodies  Response To Elevated Platelet Cytosolic Ca2+ Pathway Diagram

Diseases associated with SERPINF2 elisa kit

 Disease Name  Pubmed Publications
 Liver Diseases Antibodies  >3 publications with SERPINF2 and Liver Diseases
 Cardiovascular Diseases Antibodies  >2 publications with SERPINF2 and Cardiovascular Diseases
 Kidney Diseases Antibodies  >1 publications with SERPINF2 and Kidney Diseases
 Fibrosis Antibodies  >1 publications with SERPINF2 and Fibrosis
 Neoplasms Antibodies  >1 publications with SERPINF2 and Neoplasms
 Liver Neoplasms Antibodies  >1 publications with SERPINF2 and Liver Neoplasms
 Inflammation Antibodies  >1 publications with SERPINF2 and Inflammation
 Carcinoma, Hepatocellular Antibodies  >1 publications with SERPINF2 and Carcinoma, Hepatocellular
 Blood Coagulation Disorders Antibodies  >1 publications with SERPINF2 and Blood Coagulation Disorders

Organs/Tissues associated with SERPINF2 elisa kit

 Organ/Tissue Name  Pubmed Publications
 Liver Antibodies  >4 publications with SERPINF2 and Liver
 Heart Antibodies  >1 publications with SERPINF2 and Heart
 Kidney Antibodies  >1 publications with SERPINF2 and Kidney
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