Interleukin-2 Inducible T-Cell Kinase (ITK) ELISA Kit for Human

Name: Human ITK ELISA Kit; Interleukin-2 Inducible T-Cell Kinase; MyBioSource
Brand: MyBioSource
SKU: MBS284985
Price: 495 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS284985

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate ITK in Human serum, plasma. An antibody specific for ITK has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ITK present is bound… More details

$495 / 48 Tests +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

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Product Overview

Online Datasheet in PDF

Product Name

Interleukin-2 inducible T-cell kinase (ITK), ELISA Kit

Full Product Name

Human Interleukin-2 inducible T-cell kinase (ITK) ELISA Kit

Product Gene Name

ITK elisa kit

Product Synonym Names

EMT; LYK; MGC126257; MGC126258; PSCTK2; homolog of mouse T-cell itk/tsk|tyrosine-protein kinase ITK/TSK|tyrosine-protein kinase LYK

Species Reactivity

Human

Assay Type

Quantitative Sandwich

Samples

Serum, plasma and other biological fluids

Detection Range

0.78 ng/mL - 50 ng/mL

Sensitivity

0.39 ng/mL (mean of 6 independent assays)

Specificity

This assay has high sensitivity and excellent specificity for detection of Human ITK. No significant cross-reactivity or interference between Human ITK and analogues was observed.

Preparation and Storage

Store at 2-8 degree C.

Inter-assay Precision

Inter-assay Precision (Precision between assays) Three samples of known concentration were tested in forty separate assays to assess inter-assay precision. CV(%) = SD/meanX100. Inter-Assay: CV<12%

Intra-assay Precision

Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision. Intra-Assay: CV<8%

Molecular Weight

71,831 Da

OMIM

D13720 mRNA

3D Structure

ModBase 3D Structure for Q08881

ISO Certification

Manufactured in an ISO 9001:2015 Certified Laboratory.

Other Notes

Small volumes of ITK elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for ITK purchase

MBS284985 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Interleukin-2 inducible T-cell kinase (ITK) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing ITK. The ELISA analytical biochemical technique of the MBS284985 kit is based on ITK antibody-ITK antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect ITK antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, ITK. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for ITK. It may not necessarily be applicable to this product.

NCBI GI #

15718680

NCBI GeneID

3702

NCBI Accession #

NP_005537.3

UniProt Primary Accession #

Q08881

UniProt Secondary Accession #

Q32ML7; B2R752

UniProt Related Accession #

Q08881

GenBank Nucleotide #

NM_005546.3

NCBI Official Full Name

tyrosine-protein kinase ITK/TSK

NCBI Official Synonym Full Names

IL2 inducible T cell kinase

NCBI Official Symbol

ITK

NCBI Official Synonym Symbols

EMT; LYK; LPFS1; PSCTK2

UniProt Protein Name

Tyrosine-protein kinase ITK/TSK

UniProt Synonym Protein Names

Interleukin-2-inducible T-cell kinase; IL-2-inducible T-cell kinase; Kinase EMT; T-cell-specific kinase; Tyrosine-protein kinase Lyk

Protein Family

Tyrosine-protein kinase

UniProt Gene Name

ITK

UniProt Synonym Gene Names

EMT; LYK; IL-2-inducible T-cell kinase

NCBI Protein Information

tyrosine-protein kinase ITK/TSK

NCBI Summary for ITK

This gene encodes an intracellular tyrosine kinase expressed in T-cells. The protein contains both SH2 and SH3 domains which are often found in intracellular kinases. It is thought to play a role in T-cell proliferation and differentiation. [provided by RefSeq, Jul 2008]

UniProt Comments for ITK

ITK: an intracellular tyrosine kinase of the Tec family expressed in T-cells. Plays an essential role in regulation of the adaptive immune response. Contains both SH2 and SH3 domains and plays a role in T-cell proliferation and differentiation. Regulates the development, function and differentiation of conventional T-cells and nonconventional NKT-cells. Is recruited to the cell membrane following activation of the T-cell receptor (TCR). It is phosphorylated by LCK, followed by autophosphorylation leading to its full activation. Phosphorylates PLCG1. Itk-deficient murine T cells display defects in TCR/CD3-induced actin polymerization.

Protein type: EC 2.7.10.2; Kinase, protein; Protein kinase, TK; Protein kinase, tyrosine (non-receptor); TK group; Tec family

Chromosomal Location of Human Ortholog: 5q33.3

Cellular Component: cytosol; extrinsic component of cytoplasmic side of plasma membrane; intercellular junction; nucleus

Molecular Function: ATP binding; metal ion binding; non-membrane spanning protein tyrosine kinase activity; protein binding; receptor binding

Biological Process: activation of phospholipase C activity; adaptive immune response; cellular defense response; cytokine production; Fc-epsilon receptor signaling pathway; innate immune response; interferon-gamma production; interleukin-4 production; NK T cell differentiation; peptidyl-tyrosine autophosphorylation; regulation of cell proliferation; signal transduction; T cell activation; T cell receptor signaling pathway; transmembrane receptor protein tyrosine kinase signaling pathway

Disease: Lymphoproliferative Syndrome 1

Research Articles on ITK

1. Our results provide new insight into the effect of ITK and suboptimal T-cell receptor signaling on CD8(+) T cell function, and how these may contribute to phenotypes associated with ITK deficiency

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with ITK elisa kit

Reference Product	PubMed Publications
BTK elisa kit	>72 publications with ITK and BTK
LCK elisa kit	>43 publications with ITK and LCK
CD28 elisa kit	>41 publications with ITK and CD28

Pathways associated with ITK elisa kit

Products by Pathway	Pathway Diagram
Adaptive Immunity Signaling Pathway antibodies	Adaptive Immunity Signaling Pathway Diagram
B Cell Receptor Signaling Pathway antibodies	B Cell Receptor Signaling Pathway Diagram
Chemokine Signaling Pathway antibodies	Chemokine Signaling Pathway Diagram
Chemokine Signaling Pathway antibodies	Chemokine Signaling Pathway Diagram
Class I PI3K Signaling Events Pathway antibodies	Class I PI3K Signaling Events Pathway Diagram
Fc-epsilon Receptor I Signaling In Mast Cells Pathway antibodies	Fc-epsilon Receptor I Signaling In Mast Cells Pathway Diagram
Generation Of Second Messenger Molecules Pathway antibodies	Generation Of Second Messenger Molecules Pathway Diagram
Immune System Pathway antibodies	Immune System Pathway Diagram
Leukocyte Transendothelial Migration Pathway antibodies	Leukocyte Transendothelial Migration Pathway Diagram
Leukocyte Transendothelial Migration Pathway antibodies	Leukocyte Transendothelial Migration Pathway Diagram

Diseases associated with ITK elisa kit

Disease Name	Pubmed Publications
Neoplasms Antibodies	>30 publications with ITK and Neoplasms
Pain Antibodies	>5 publications with ITK and Pain
Prostatic Neoplasms Antibodies	>3 publications with ITK and Prostatic Neoplasms
Liver Neoplasms Antibodies	>3 publications with ITK and Liver Neoplasms
Skin Neoplasms Antibodies	>2 publications with ITK and Skin Neoplasms
Lymphoma, B-Cell Antibodies	>2 publications with ITK and Lymphoma, B-Cell
Carcinoma, Squamous Cell Antibodies	>1 publications with ITK and Carcinoma, Squamous Cell
Adenoma Antibodies	>1 publications with ITK and Adenoma
Heart Defects, Congenital Antibodies	>1 publications with ITK and Heart Defects, Congenital
Neurodegenerative Diseases Antibodies	>1 publications with ITK and Neurodegenerative Diseases

Organs/Tissues associated with ITK elisa kit

Organ/Tissue Name	Pubmed Publications
Blood Antibodies	>189 publications with ITK and Blood
Thymus Antibodies	>26 publications with ITK and Thymus
Lung Antibodies	>17 publications with ITK and Lung
Brain Antibodies	>14 publications with ITK and Brain
Spleen Antibodies	>13 publications with ITK and Spleen
Intestine Antibodies	>8 publications with ITK and Intestine
Prostate Antibodies	>6 publications with ITK and Prostate
Lymph Node Antibodies	>6 publications with ITK and Lymph Node
Heart Antibodies	>5 publications with ITK and Heart
Trachea Antibodies	>2 publications with ITK and Trachea