Dermatopontin (DPT) ELISA Kit for Human

Dermatopontin is an extracellular matrix protein with possible functions in cell-matrix interactions and matrix assembly. Dermatopontin is postulated to modify the behavior of TGF-beta through interaction with decorin. Seems to mediate adhesion by cell surface integrin binding. May serve as a communication link between the dermal fibroblast cell surface and its extracellular matrix environment. Enhances TGFB1 activity. Inhibits cell proliferation. Expressed in fibroblasts, heart, skeletal muscle, brain and pancreas. Expressed at an intermediate level in lung and kidney, and at a low level in liver and placenta. Expressed at a lower level in fibroblasts from hypertrophic scar lesional skin and in fibroblasts from patients with systemic sclerosis than in normal skin fibroblasts.

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate DPT in samples. An antibody specific for DPT has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyDPT present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DPT is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DPT bound in the initial step. The color development is stopped and the intensity of the color is measured.

Small volumes of DPT elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS285107 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Dermatopontin (DPT) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing DPT. The ELISA analytical biochemical technique of the MBS285107 kit is based on DPT antibody-DPT antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect DPT antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, DPT. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).