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UTP--Glucose-1-Phosphate Uridylyltransferase (UGP2) ELISA Kit for Mouse

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS285168

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate UGP2 in samples. An antibody specific for UGP2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyUGP2 present is bound by the… More details

$495 / 48 Tests +1 FREE 8GB USB

Product Documentation

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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
UTP--glucose-1-phosphate uridylyltransferase (UGP2), ELISA Kit
Full Product Name
Mouse UTP--glucose-1-phosphate uridylyltransferase (UGP2) ELISA Kit
Product Gene Name
UGP2 elisa kit
Product Synonym Names
UDPG; UDPGP2; UGPP2; pHC379; UDP-glucose diphosphorylase|UGPase 2|UTP--glucose-1-phosphate uridylyltransferase 2|UTP-glucose-1-phosphate uridyltransferase|uridyl diphosphate glucose pyrophosphorylas
Species Reactivity
Mouse
Assay Type
Sandwich
Samples
Serum, Plasma, Other biological fluids
Specificity
This assay has high sensitivity and excellent specificity for detection of Mouse UGP2. No significant cross-reactivity or interference between Mouse UGP2 and analogues was observed.
Preparation and Storage
Store at 2-8 degree C.
Molecular Weight
55,716 Da
3D Structure
ModBase 3D Structure for Q91ZJ5
Sample Volume
1-200 uL
Precision
Intra-assay Precision (Precision within an assay)
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%) = SD/meanX100
Intra-Assay: CV
Inter-Assay: CV
Detection Wavelength
450 nm
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.

Other Notes

Small volumes of UGP2 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for UGP2 purchase

MBS285168 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the UTP--glucose-1-phosphate uridylyltransferase (UGP2) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing UGP2. The ELISA analytical biochemical technique of the MBS285168 kit is based on UGP2 antibody-UGP2 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect UGP2 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, UGP2. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for UGP2. It may not necessarily be applicable to this product.

NCBI GI #
594591591
NCBI GeneID
216558
NCBI Accession #
NP_001277563.1
UniProt Primary Accession #
Q91ZJ5
UniProt Secondary Accession #
Q8R3D2
UniProt Related Accession #
Q91ZJ5
GenBank Nucleotide #
NM_001290634.1
NCBI Official Full Name
UTP--glucose-1-phosphate uridylyltransferase isoform 2
NCBI Official Synonym Full Names
UDP-glucose pyrophosphorylase 2
NCBI Official Symbol
Ugp2
NCBI Official Synonym Symbols
UDPGP; UGPase
UniProt Protein Name
UTP--glucose-1-phosphate uridylyltransferase
UniProt Synonym Protein Names
UDP-glucose pyrophosphorylase; UDPGP; UGPase
Protein Family
UTP--glucose-1-phosphate uridylyltransferase
UniProt Gene Name
Ugp2
UniProt Synonym Gene Names
UDPGP; UGPase
NCBI Protein Information
UTP--glucose-1-phosphate uridylyltransferase

UniProt Comments for UGP2

UGP2: Plays a central role as a glucosyl donor in cellular metabolic pathways. Belongs to the UDPGP type 1 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Carbohydrate Metabolism - amino sugar and nucleotide sugar; Carbohydrate Metabolism - galactose; Carbohydrate Metabolism - pentose and glucuronate interconversions; Carbohydrate Metabolism - starch and sucrose; EC 2.7.7.9; Transferase

Chromosomal Location of Human Ortholog: 11 A3.1|11 13.89 cM

Cellular Component: cytoplasm; extracellular exosome; nucleus

Molecular Function: glucose binding; identical protein binding; metal ion binding; nucleotidyltransferase activity; pyrimidine ribonucleotide binding; transferase activity; UTP:glucose-1-phosphate uridylyltransferase activity

Biological Process: glucose 1-phosphate metabolic process; glycogen metabolic process; metabolic process; UDP-glucose metabolic process
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Research Articles on UGP2

1. GYS1 regulation by HIF plays a central role in the hypoxic accumulation of glycogen, and hypoxia also upregulates the expression of UTP:glucose-1-phosphate urydylyltransferase (UGP2) and 1,4-alpha glucan branching enzyme (GBE1)

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with UGP2 elisa kit

 Products by Pathway  Pathway Diagram
 Amino Sugar And Nucleotide Sugar Metabolism Pathway antibodies  Amino Sugar And Nucleotide Sugar Metabolism Pathway Diagram
 Amino Sugar And Nucleotide Sugar Metabolism Pathway antibodies  Amino Sugar And Nucleotide Sugar Metabolism Pathway Diagram
 Ascorbate Biosynthesis, Animals, Glucose-1P => Ascorbate Pathway antibodies  Ascorbate Biosynthesis, Animals, Glucose-1P => Ascorbate Pathway Diagram
 Ascorbate Biosynthesis, Animals, Glucose-1P => Ascorbate Pathway antibodies  Ascorbate Biosynthesis, Animals, Glucose-1P => Ascorbate Pathway Diagram
 Biological Oxidations Pathway antibodies  Biological Oxidations Pathway Diagram
 Circadian Exercise Pathway antibodies  Circadian Exercise Pathway Diagram
 Diurnally Regulated Genes With Circadian Orthologs Pathway antibodies  Diurnally Regulated Genes With Circadian Orthologs Pathway Diagram
 Formation Of The Active Cofactor, UDP-glucuronate Pathway antibodies  Formation Of The Active Cofactor, UDP-glucuronate Pathway Diagram
 Galactose Metabolism Pathway antibodies  Galactose Metabolism Pathway Diagram
 Galactose Metabolism Pathway antibodies  Galactose Metabolism Pathway Diagram

Diseases associated with UGP2 elisa kit

 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >2 publications with UGP2 and Neoplasms
 Adenoma Antibodies  >1 publications with UGP2 and Adenoma
 Lung Neoplasms Antibodies  >1 publications with UGP2 and Lung Neoplasms
 Adenoma, Liver Cell Antibodies  >1 publications with UGP2 and Adenoma, Liver Cell
 Liver Neoplasms Antibodies  >1 publications with UGP2 and Liver Neoplasms

Organs/Tissues associated with UGP2 elisa kit

 Organ/Tissue Name  Pubmed Publications
 Muscle Antibodies  >2 publications with UGP2 and Muscle
 Liver Antibodies  >2 publications with UGP2 and Liver
 Bone Antibodies  >1 publications with UGP2 and Bone
 Prostate Antibodies  >1 publications with UGP2 and Prostate
 Eye Antibodies  >1 publications with UGP2 and Eye
 Lung Antibodies  >1 publications with UGP2 and Lung
 Blood Antibodies  >1 publications with UGP2 and Blood
 Bone Marrow Antibodies  >1 publications with UGP2 and Bone Marrow
 Brain Antibodies  >1 publications with UGP2 and Brain
 Bladder Antibodies  >1 publications with UGP2 and Bladder
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