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Drebrin (DBN1) ELISA Kit for Mouse

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS285207

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate DBN1 in samples. An antibody specific for DBN1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyDBN1 present is bound by the… More details

$495 / 48 Tests +1 FREE 8GB USB

Product Documentation

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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Drebrin (DBN1), ELISA Kit
Full Product Name
Mouse Drebrin (DBN1) ELISA Kit
Product Gene Name
DBN1 elisa kit
Product Synonym Names
D0S117E; DKFZp434D064; drebrin E|drebrin E2|drebrin-1
Species Reactivity
Mouse
Assay Type
Sandwich
Samples
Serum, Plasma, Other biological fluids
Specificity
This assay has high sensitivity and excellent specificity for detection of Mouse DBN1. No significant cross-reactivity or interference between Mouse DBN1 and analogues was observed.
Preparation and Storage
Store at 2-8 degree C.
Molecular Weight
72,416 Da
3D Structure
ModBase 3D Structure for Q9QXS6
Sample Volume
1-200 uL
Precision
Intra-assay Precision (Precision within an assay)
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%) = SD/meanX100
Intra-Assay: CV
Inter-Assay: CV
Detection Wavelength
450 nm
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.

Other Notes

Small volumes of DBN1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for DBN1 purchase

MBS285207 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Drebrin (DBN1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing DBN1. The ELISA analytical biochemical technique of the MBS285207 kit is based on DBN1 antibody-DBN1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect DBN1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, DBN1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for DBN1. It may not necessarily be applicable to this product.

NCBI GI #
293651556
NCBI GeneID
56320
NCBI Accession #
NP_001170842.1
UniProt Primary Accession #
Q9QXS6
UniProt Secondary Accession #
Q3V234; Q922X1; Q9QXS5; A2CG16
UniProt Related Accession #
Q9QXS6
GenBank Nucleotide #
NM_001177371.1
NCBI Official Full Name
drebrin isoform 1
NCBI Official Synonym Full Names
drebrin 1
NCBI Official Symbol
Dbn1
UniProt Protein Name
Drebrin
UniProt Synonym Protein Names
Developmentally-regulated brain protein
Protein Family
Drebrin
UniProt Gene Name
Dbn1
UniProt Synonym Gene Names
Drba
NCBI Protein Information
drebrin

UniProt Comments for DBN1

DBN1: a cytoplasmic actin-binding protein thought to play a role in the process of neuronal growth. It is a member of the drebrin family of proteins that are developmentally regulated in the brain. A decrease in the amount of this protein in the brain has been implicated as a possible contributing factor in the pathogenesis of memory disturbance in Alzheimer's disease. Expressed in brain neurons, heart, placenta, skeletal muscle, kidney and pancreas. Two alternatively spliced isoforms have been described.

Protein type: Actin-binding; Cytoskeletal; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 13 B1|13 30.06 cM

Cellular Component: actin cytoskeleton; actin filament; asymmetric synapse; axonal growth cone; axonal spine; cell junction; cell projection; cell soma; cytoplasm; dendritic filopodium; dendritic shaft; dendritic spine; excitatory synapse; filopodium; gap junction; growth cone; intercellular junction; intracellular; lamellipodium; membrane; neuron spine; perinuclear region of cytoplasm; plasma membrane; postsynaptic density; postsynaptic membrane

Molecular Function: actin binding; actin filament binding; cadherin binding; cytoskeletal protein binding; profilin binding; protein binding

Biological Process: actin filament organization; cell communication by chemical coupling; cell communication by electrical coupling; cell differentiation; cellular response to transforming growth factor beta stimulus; cellular response to tumor necrosis factor; generation of neurons; maintenance of cellular protein localization; modulation of inhibitory postsynaptic potential; multicellular organism development; negative regulation of actin filament bundle formation; negative regulation of actin-dependent ATPase activity; negative regulation of locomotion involved in locomotory behavior; negative regulation of muscle filament sliding; nervous system development; positive regulation of axon extension; positive regulation of cell-substrate adhesion; positive regulation of excitatory postsynaptic potential; positive regulation of neuron projection development; positive regulation of substrate adhesion-dependent cell spreading; prepulse inhibition; regulation of actin filament polymerization; regulation of filopodium formation
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Research Articles on DBN1

1. these findings suggest that during cell migration drebrin is involved in retraction processes but not in lamellipodia formation. The novel, sizable juxtanuclear drebrin-enriched zone remains to be characterized in detail with respect to its molecular assembly and functions.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Diseases associated with DBN1 elisa kit

 Disease Name  Pubmed Publications
 Lung Diseases Antibodies  >2 publications with DBN1 and Lung Diseases
 Neoplasms Antibodies  >2 publications with DBN1 and Neoplasms
 Bone Marrow Diseases Antibodies  >1 publications with DBN1 and Bone Marrow Diseases
 Lung Neoplasms Antibodies  >1 publications with DBN1 and Lung Neoplasms
 Lymphoma Antibodies  >1 publications with DBN1 and Lymphoma
 Carcinoma Antibodies  >1 publications with DBN1 and Carcinoma

Organs/Tissues associated with DBN1 elisa kit

 Organ/Tissue Name  Pubmed Publications
 Muscle Antibodies  >3 publications with DBN1 and Muscle
 Lung Antibodies  >2 publications with DBN1 and Lung
 Bone Marrow Antibodies  >1 publications with DBN1 and Bone Marrow
 Bone Antibodies  >1 publications with DBN1 and Bone
 Embryonic Tissue Antibodies  >1 publications with DBN1 and Embryonic Tissue
 Brain Antibodies  >1 publications with DBN1 and Brain
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