Glucagon (GC) ELISA Kit for Human

Name: Human GC ELISA Kit; Glucagon; MyBioSource
Brand: MyBioSource
SKU: MBS285271
Price: 545 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS285271

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate GCG in samples. An antibody specific for GCG has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyGCG present is bound by the… More details

$545 / 48 Tests +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

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Product Overview

Online Datasheet in PDF

Product Name

Glucagon (GC), ELISA Kit

Full Product Name

Human Glucagon (GC) ELISA Kit

Product Gene Name

GC elisa kit

Product Synonym Names

GLP1; GLP2; GRPP; glicentin-related polypeptide|glucagon-like peptide 1|glucagon-like peptide

Species Reactivity

Human

Assay Type

Sandwich

Samples

Serum, Plasma, Other biological fluids

Specificity

This assay has high sensitivity and excellent specificity for detection of Human GCG. No significant cross-reactivity or interference between Human GCG and analogues was observed.

Preparation and Storage

Store at 2-8 degree C.

Molecular Weight

20,909 Da

OMIM

J04040 mRNA

3D Structure

ModBase 3D Structure for P01275

Sample Volume

1-200 uL

Precision

Intra-assay Precision (Precision within an assay)
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%) = SD/meanX100
Intra-Assay: CV
Inter-Assay: CV

Detection Wavelength

450 nm

ISO Certification

Manufactured in an ISO 9001:2015 Certified Laboratory.

Other Notes

Small volumes of GC elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for GC purchase

MBS285271 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Glucagon (GC) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing GC. The ELISA analytical biochemical technique of the MBS285271 kit is based on GC antibody-GC antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect GC antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, GC. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for GC. It may not necessarily be applicable to this product.

NCBI GI #

4503945

NCBI GeneID

2641

NCBI Accession #

NP_002045.1

UniProt Primary Accession #

P01275

UniProt Secondary Accession #

Q53TP6; A6NN65

UniProt Related Accession #

P01275

GenBank Nucleotide #

NM_002054.4

NCBI Official Full Name

glucagon preproprotein

NCBI Official Synonym Full Names

glucagon

NCBI Official Symbol

GCG

NCBI Official Synonym Symbols

GLP1; GLP2; GRPP; GLP-1

UniProt Protein Name

Glucagon

UniProt Synonym Protein Names

GlicentinGlicentin-related polypeptide; GRPP

Protein Family

Envelope glycoprotein

UniProt Gene Name

GCG

UniProt Synonym Gene Names

GRPP; OXM; OXY; GLP-1; GLP-1(7-37); GLP-1(7-36); GLP-2

NCBI Protein Information

glucagon

NCBI Summary for GC

The protein encoded by this gene is actually a preproprotein that is cleaved into four distinct mature peptides. One of these, glucagon, is a pancreatic hormone that counteracts the glucose-lowering action of insulin by stimulating glycogenolysis and gluconeogenesis. Glucagon is a ligand for a specific G-protein linked receptor whose signalling pathway controls cell proliferation. Two of the other peptides are secreted from gut endocrine cells and promote nutrient absorption through distinct mechanisms. Finally, the fourth peptide is similar to glicentin, an active enteroglucagon. [provided by RefSeq, Jul 2008]

UniProt Comments for GC

GCG: Glucagon plays a key role in glucose metabolism and homeostasis. Regulates blood glucose by increasing gluconeogenesis and decreasing glycolysis. A counterregulatory hormone of insulin, raises plasma glucose levels in response to insulin-induced hypoglycemia. Plays an important role in initiating and maintaining hyperglycemic conditions in diabetes. Glucagon release is stimulated by hypoglycemia and inhibited by hyperglycemia, insulin, and somatostatin. GLP-1 and GLP-2 are induced in response to nutrient ingestion. Glucagon is secreted in the A cells of the islets of Langerhans. GLP-1, GLP-2, oxyntomodulin and glicentin are secreted from enteroendocrine cells throughout the gastrointestinal tract. GLP1 and GLP2 are also secreted in selected neurons in the brain. Belongs to the glucagon family.

Protein type: Hormone; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 2q24.2

Cellular Component: endoplasmic reticulum lumen; extracellular region; extracellular space; plasma membrane

Molecular Function: glucagon receptor binding; hormone activity; identical protein binding; protein binding; receptor binding

Biological Process: cell proliferation; feeding behavior; G-protein coupled receptor signaling pathway; G-protein signaling, coupled to cAMP nucleotide second messenger; negative regulation of apoptosis; positive regulation of cAMP biosynthetic process; positive regulation of histone H3-K4 methylation; positive regulation of peptidyl-serine phosphorylation; positive regulation of protein binding; positive regulation of protein kinase activity; regulation of insulin secretion; response to starvation; signal transduction

Research Articles on GC

1. the solvent exposure of the two Phe sites along the glucagon sequence was determined, showing that 4F-Phe6 was fully solvent exposed and 4F-Phe22 was only partially exposed

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with GC elisa kit

Reference Product	PubMed Publications
GLP1R elisa kit	>5 publications with GC and GLP1R
GCGR elisa kit	>4 publications with GC and GCGR
GLP2R elisa kit	>3 publications with GC and GLP2R

Pathways associated with GC elisa kit

Products by Pathway	Pathway Diagram
Class B/2 (Secretin Family Receptors) Pathway antibodies	Class B/2 (Secretin Family Receptors) Pathway Diagram
FOXA1 Transcription Factor Network Pathway antibodies	FOXA1 Transcription Factor Network Pathway Diagram
G Alpha (q) Signalling Events Pathway antibodies	G Alpha (q) Signalling Events Pathway Diagram
G Alpha (s) Signalling Events Pathway antibodies	G Alpha (s) Signalling Events Pathway Diagram
GPCR Downstream Signaling Pathway antibodies	GPCR Downstream Signaling Pathway Diagram
GPCR Ligand Binding Pathway antibodies	GPCR Ligand Binding Pathway Diagram
Gastrin-CREB Signalling Pathway Via PKC And MAPK antibodies	Gastrin-CREB Signalling Pathway Via PKC And MAPK Diagram
Glucagon Signaling In Metabolic Regulation Pathway antibodies	Glucagon Signaling In Metabolic Regulation Pathway Diagram
Glucagon-type Ligand Receptors Pathway antibodies	Glucagon-type Ligand Receptors Pathway Diagram
Incretin Synthesis, Secretion, And Inactivation Pathway antibodies	Incretin Synthesis, Secretion, And Inactivation Pathway Diagram

Diseases associated with GC elisa kit

Disease Name	Pubmed Publications
Muscular Diseases Antibodies	>70 publications with GC and Muscular Diseases
Diabetes Mellitus Antibodies	>30 publications with GC and Diabetes Mellitus
Vascular Diseases Antibodies	>15 publications with GC and Vascular Diseases
Kidney Diseases Antibodies	>11 publications with GC and Kidney Diseases
Obesity Antibodies	>11 publications with GC and Obesity
Insulin Resistance Antibodies	>10 publications with GC and Insulin Resistance
Hyperglycemia Antibodies	>8 publications with GC and Hyperglycemia
Heart Diseases Antibodies	>8 publications with GC and Heart Diseases
Liver Diseases Antibodies	>7 publications with GC and Liver Diseases
Drug Toxicity Antibodies	>7 publications with GC and Drug Toxicity

Organs/Tissues associated with GC elisa kit

Organ/Tissue Name	Pubmed Publications
Pancreas Antibodies	>26 publications with GC and Pancreas
Intestine Antibodies	>13 publications with GC and Intestine