Neuro-D4 (DPF1) ELISA Kit for Human

DPF1 is a member of the d4 domain family, characterized by a zinc finger-like structural motif. This protein functions as a transcription factor which is necessary for the apoptotic response following deprivation of survival factors. It likely serves a regulatory role in rapid hematopoietic cell growth and turnover. Chestkov et al. (1996) described human DPF1, which they called Neuro-D4, a conserved gene encoding a 353-amino acid neural-specific zinc finger protein. A cysteine- and histidine-rich region of the C terminus distinguishes members of the D4 family, including Neuro-D4, from other zinc finger proteins. The human and rat Neuro-D4 cDNAs are 93% identical, and there are only 3 amino acid differences between the proteins. Different alternatively spliced cDNAs were observed.

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate DPF1 in samples. An antibody specific for DPF1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyDPF1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DPF1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DPF1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Small volumes of DPF1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS285302 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the neuro-d4 (DPF1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing DPF1. The ELISA analytical biochemical technique of the MBS285302 kit is based on DPF1 antibody-DPF1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect DPF1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, DPF1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).