Tryptase Beta-2 (TPSB2) ELISA Kit for Human

Name: Human TPSB2 ELISA Kit; Tryptase Beta-2; MyBioSource
Brand: MyBioSource
SKU: MBS285499
Price: 495 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS285499

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate TPSB2 in samples. An antibody specific for TPSB2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTPSB2 present is bound by the… More details

$495 / 48 Tests +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Request Current Manual

Product Overview

Online Datasheet in PDF

Product Name

Tryptase beta-2 (TPSB2), ELISA Kit

Full Product Name

Human Tryptase beta-2 (TPSB2) ELISA Kit

Product Gene Name

TPSB2 elisa kit

Product Synonym Names

Species Reactivity

Human

Assay Type

Sandwich

Samples

Serum, Plasma, Other biological fluids

Specificity

This assay has high sensitivity and excellent specificity for detection of Human TPSB2. No significant cross-reactivity or interference between Human TPSB2 and analogues was observed.

Preparation and Storage

Store at 2-8 degree C.

Molecular Weight

30,515 Da

OMIM

M37488 mRNA

3D Structure

ModBase 3D Structure for P20231

Sample Volume

1-200 uL

Precision

Intra-assay Precision (Precision within an assay)
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%) = SD/meanX100
Intra-Assay: CV
Inter-Assay: CV

Detection Wavelength

450 nm

ISO Certification

Manufactured in an ISO 9001:2015 Certified Laboratory.

Other Notes

Small volumes of TPSB2 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for TPSB2 purchase

MBS285499 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Tryptase beta-2 (TPSB2) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing TPSB2. The ELISA analytical biochemical technique of the MBS285499 kit is based on TPSB2 antibody-TPSB2 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect TPSB2 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, TPSB2. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for TPSB2. It may not necessarily be applicable to this product.

NCBI GI #

13775595

NCBI GeneID

7177

NCBI Accession #

NP_003285.2

UniProt Primary Accession #

P20231

UniProt Secondary Accession #

O95827; Q15664; Q9UQI6; Q9UQI7; D2E6S0; D2E6S2

UniProt Related Accession #

Q15661

GenBank Nucleotide #

NM_003294.3

NCBI Official Full Name

tryptase alpha/beta-1

NCBI Official Synonym Full Names

tryptase alpha/beta 1

NCBI Official Symbol

TPSAB1

NCBI Official Synonym Symbols

TPS1; TPS2; TPSB1

UniProt Protein Name

Tryptase beta-2

UniProt Synonym Protein Names

Tryptase II

Protein Family

Tryptase

UniProt Gene Name

TPSB2

UniProt Synonym Gene Names

TPS2; Tryptase-2

NCBI Protein Information

tryptase alpha/beta-1

NCBI Summary for TPSB2

Tryptases comprise a family of trypsin-like serine proteases, the peptidase family S1. Tryptases are enzymatically active only as heparin-stabilized tetramers, and they are resistant to all known endogenous proteinase inhibitors. Several tryptase genes are clustered on chromosome 16p13.3. These genes are characterized by several distinct features. They have a highly conserved 3' UTR and contain tandem repeat sequences at the 5' flank and 3' UTR which are thought to play a role in regulation of the mRNA stability. These genes have an intron immediately upstream of the initiator Met codon, which separates the site of transcription initiation from protein coding sequence. This feature is characteristic of tryptases but is unusual in other genes. The alleles of this gene exhibit an unusual amount of sequence variation, such that the alleles were once thought to represent two separate genes, alpha and beta 1. Beta tryptases appear to be the main isoenzymes expressed in mast cells; whereas in basophils, alpha tryptases predominate. Tryptases have been implicated as mediators in the pathogenesis of asthma and other allergic and inflammatory disorders. [provided by RefSeq, Jul 2008]

UniProt Comments for TPSB2

Tryptase is the major neutral protease present in mast cells and is secreted upon the coupled activation-degranulation response of this cell type. May play a role in innate immunity.

Research Articles on TPSB2

1. Basal serum tryptase in children with actual drug hypersensitivity is not a risk factor for severe systemic reactions

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with TPSB2 elisa kit

Products by Pathway	Pathway Diagram
Activation Of Matrix Metalloproteinases Pathway antibodies	Activation Of Matrix Metalloproteinases Pathway Diagram
Degradation Of The Extracellular Matrix Pathway antibodies	Degradation Of The Extracellular Matrix Pathway Diagram
Extracellular Matrix Organization Pathway antibodies	Extracellular Matrix Organization Pathway Diagram

Diseases associated with TPSB2 elisa kit

Disease Name	Pubmed Publications
Inflammation Antibodies	>26 publications with TPSB2 and Inflammation
Immune System Diseases Antibodies	>19 publications with TPSB2 and Immune System Diseases
Neoplasms Antibodies	>13 publications with TPSB2 and Neoplasms
Necrosis Antibodies	>4 publications with TPSB2 and Necrosis
Hyperplasia Antibodies	>4 publications with TPSB2 and Hyperplasia
Skin Abnormalities Antibodies	>3 publications with TPSB2 and Skin Abnormalities
Edema Antibodies	>3 publications with TPSB2 and Edema
Carcinoma Antibodies	>3 publications with TPSB2 and Carcinoma
Muscular Diseases Antibodies	>1 publications with TPSB2 and Muscular Diseases
Weight Loss Antibodies	>1 publications with TPSB2 and Weight Loss

Organs/Tissues associated with TPSB2 elisa kit

Organ/Tissue Name	Pubmed Publications
Bone Antibodies	>20 publications with TPSB2 and Bone
Lung Antibodies	>19 publications with TPSB2 and Lung
Connective Tissue Antibodies	>15 publications with TPSB2 and Connective Tissue
Intestine Antibodies	>8 publications with TPSB2 and Intestine
Nerve Antibodies	>5 publications with TPSB2 and Nerve
Liver Antibodies	>5 publications with TPSB2 and Liver
Kidney Antibodies	>3 publications with TPSB2 and Kidney
Stomach Antibodies	>3 publications with TPSB2 and Stomach
Uterus Antibodies	>2 publications with TPSB2 and Uterus
Lymph Node Antibodies	>2 publications with TPSB2 and Lymph Node