Interleukin Enhancer-Binding Factor 3 (ILF3) ELISA Kit for Rat

Name: Rat ILF3 ELISA Kit; Interleukin Enhancer-Binding Factor 3; MyBioSource
Brand: MyBioSource
SKU: MBS285519
Price: 495 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS285519

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate ILF3 in samples. An antibody specific for ILF3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyILF3 present is bound by the… More details

$495 / 48 Tests +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Request Current Manual

Product Overview

Online Datasheet in PDF

Product Name

Interleukin enhancer-binding factor 3 (ILF3), ELISA Kit

Full Product Name

Rat Interleukin enhancer-binding factor 3 (ILF3) ELISA Kit

Product Gene Name

ILF3 elisa kit

Product Synonym Names

CBTF; DRBF; DRBP76; MMP4; MPHOSPH4; MPP4; NF-AT-90; NF110; NF90; NFAR; NFAR-1; NFAR2; TCP110; TCP80; M-phase phosphoprotein 4|double-stranded RNA-binding protein; 76 kD|dsRNA binding protein NFAR-2/

Species Reactivity

Rat

Assay Type

Sandwich

Samples

Serum, Plasma, Other biological fluids

Specificity

This assay has high sensitivity and excellent specificity for detection of Rat ILF3. No significant cross-reactivity or interference between Rat ILF3 and analogues was observed.

Preparation and Storage

Store at 2-8 degree C.

Molecular Weight

97,680 Da

3D Structure

ModBase 3D Structure for Q9JIL3

Sample Volume

1-200 uL

Precision

Intra-assay Precision (Precision within an assay)
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%) = SD/meanX100
Intra-Assay: CV
Inter-Assay: CV

Detection Wavelength

450 nm

ISO Certification

Manufactured in an ISO 9001:2015 Certified Laboratory.

Other Notes

Small volumes of ILF3 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for ILF3 purchase

MBS285519 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Interleukin enhancer-binding factor 3 (ILF3) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing ILF3. The ELISA analytical biochemical technique of the MBS285519 kit is based on ILF3 antibody-ILF3 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect ILF3 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, ILF3. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for ILF3. It may not necessarily be applicable to this product.

NCBI GI #

16758150

NCBI GeneID

84472

NCBI Accession #

NP_445864.1

UniProt Primary Accession #

Q9JIL3

UniProt Related Accession #

Q9JIL3

GenBank Nucleotide #

NM_053412.1

NCBI Official Full Name

interleukin enhancer-binding factor 3

NCBI Official Synonym Full Names

interleukin enhancer binding factor 3

NCBI Official Symbol

Ilf3

UniProt Protein Name

Interleukin enhancer-binding factor 3

Protein Family

Interleukin enhancer-binding factor

UniProt Gene Name

Ilf3

NCBI Protein Information

interleukin enhancer-binding factor 3

NCBI Summary for ILF3

nuclear protein that interacts with and regulates the activity of protein-arginine methyltransferase I [RGD, Feb 2006]

UniProt Comments for ILF3

NFAT90: a double-stranded RNA (dsRNA)-binding protein implicated in the regulation of gene expression . Phosphorylated in a double-stranded RNA-dependent manner possibly by PKR. Five different splice-variant isoforms have been described.

Protein type: DNA-binding; Nucleolus; RNA-binding; Transcription factor

Chromosomal Location of Human Ortholog: 8q13

Cellular Component: cytoplasm; intracellular ribonucleoprotein complex; membrane; mitochondrion; nucleolus; nucleoplasm; nucleus

Molecular Function: DNA binding; DNA binding transcription factor activity; double-stranded RNA binding; enzyme binding; protein binding; RNA binding

Biological Process: defense response to virus; negative regulation of transcription, DNA-dependent; negative regulation of translation; negative regulation of viral genome replication; positive regulation of transcription, DNA-templated; protein amino acid methylation; protein amino acid phosphorylation; regulation of transcription, DNA-templated; transcription, DNA-dependent

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Diseases associated with ILF3 elisa kit

Disease Name	Pubmed Publications
Neoplasms Antibodies	>6 publications with ILF3 and Neoplasms
Myocardial Infarction Antibodies	>4 publications with ILF3 and Myocardial Infarction
Heart Diseases Antibodies	>3 publications with ILF3 and Heart Diseases
Skin Diseases Antibodies	>2 publications with ILF3 and Skin Diseases
Kidney Diseases Antibodies	>2 publications with ILF3 and Kidney Diseases
Breast Neoplasms Antibodies	>2 publications with ILF3 and Breast Neoplasms
Leukemia Antibodies	>2 publications with ILF3 and Leukemia
Adenocarcinoma Antibodies	>1 publications with ILF3 and Adenocarcinoma
Disease Models, Animal Antibodies	>1 publications with ILF3 and Disease Models, Animal
Endometrial Neoplasms Antibodies	>1 publications with ILF3 and Endometrial Neoplasms

Organs/Tissues associated with ILF3 elisa kit

Organ/Tissue Name	Pubmed Publications
Kidney Antibodies	>4 publications with ILF3 and Kidney
Lung Antibodies	>2 publications with ILF3 and Lung
Heart Antibodies	>1 publications with ILF3 and Heart