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Adapter Molecule Crk (CRK) ELISA Kit for Rat

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS285535

v-CRK avian sarcoma virus CT10-homolog-like contains one SH2 domain and two SH3 domains. CRKL has been shown to activate the RAS and JUN kinase signaling pathways and transform fibroblasts in a RAS-dependent fashion. It is a substrate of the BCR-ABL tyrosine… More details

$495 / 48 Tests +1 FREE 8GB USB

Product Documentation

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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Adapter molecule crk, ELISA Kit
Full Product Name
Rat Adapter molecule crk (CRK) ELISA Kit
Product Gene Name
CRK elisa kit
Product Synonym Names
CRKII; avian sarcoma virus CT10 (v-crk) oncogene homolog|v-crk avian sarcoma virus CT10 oncogene homolog|v-crk sarcoma virus CT10 oncogene homolog
Species Reactivity
Rat
Assay Type
Sandwich
Samples
Serum, Plasma, Other biological fluids
Specificity
This assay has high sensitivity and excellent specificity for detection of Rat CRK. No significant cross-reactivity or interference between Rat CRK and analogues was observed.
Preparation and Storage
Store at 2-8 degree C.
Molecular Weight
22,863 Da
3D Structure
ModBase 3D Structure for Q63768
Sample Volume
1-200 uL
Precision
Intra-assay Precision (Precision within an assay)
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%) = SD/meanX100
Intra-Assay: CV
Inter-Assay: CV
Detection Wavelength
450 nm
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.

Other Notes

Small volumes of CRK elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CRK purchase

MBS285535 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Adapter molecule crk, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing CRK. The ELISA analytical biochemical technique of the MBS285535 kit is based on CRK antibody-CRK antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect CRK antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, CRK. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for CRK. It may not necessarily be applicable to this product.

NCBI GI #
9506515
NCBI GeneID
54245
NCBI Accession #
NP_062175.1
UniProt Primary Accession #
Q63768
UniProt Related Accession #
Q63768
GenBank Nucleotide #
NM_019302.1
NCBI Official Full Name
adapter molecule crk
NCBI Official Synonym Full Names
CRK proto-oncogene, adaptor protein
NCBI Official Symbol
Crk
NCBI Official Synonym Symbols
Crko; CrkII
UniProt Protein Name
Adapter molecule crk
UniProt Synonym Protein Names
Proto-oncogene c-Crk; p38
Protein Family
Adapter molecule
UniProt Gene Name
Crk
UniProt Synonym Gene Names
Crko
NCBI Protein Information
adapter molecule crk

NCBI Summary for CRK

adaptor protein that interacts with tyrosine-phosporylated proteins to transduce cellular signals in multiple pathways [RGD, Feb 2006]

UniProt Comments for CRK

CRK: an adaptor protein with an SH2-SH3-SH3 domain structure. Recruits cytoplasmic proteins through SH2-phospho-tyrosine interaction. Phosphorylated by Abl, IGF-IR and EGFR. Phosphorylation induces a change in intramolecular folding and SH2 interactions, causing its rapid dissociation from the tyrosine kinase complex.

Protein type: Adaptor/scaffold; Motility/polarity/chemotaxis; Oncoprotein

Chromosomal Location of Human Ortholog: 10q24

Cellular Component: actin cytoskeleton; cytosol; extracellular exosome; membrane raft; plasma membrane; protein complex

Molecular Function: cytoskeletal protein binding; enzyme binding; ephrin receptor binding; insulin-like growth factor receptor binding; phosphotyrosine binding; protein binding; protein binding, bridging; protein domain specific binding; protein phosphorylated amino acid binding; protein self-association; SH2 domain binding; SH3 domain binding; SH3/SH2 adaptor activity

Biological Process: activation of JNK activity; activation of MAPKK activity; cellular response to endothelin; cellular response to insulin-like growth factor stimulus; cellular response to nitric oxide; cellular response to transforming growth factor beta stimulus; ephrin receptor signaling pathway; negative regulation of cell motility; negative regulation of natural killer cell mediated cytotoxicity; positive regulation of cell growth; positive regulation of smooth muscle cell migration; positive regulation of substrate adhesion-dependent cell spreading; regulation of actin cytoskeleton organization; regulation of cell motility; regulation of cell shape; regulation of cellular component movement; regulation of GTPase activity; regulation of protein binding; regulation of Rac protein signal transduction; regulation of signal transduction; response to cholecystokinin; response to hydrogen peroxide; response to peptide; response to yeast; SH2 domain-mediated complex assembly
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Research Articles on CRK

1. overexpression of miR-126 significantly inhibited the activation and migration of hepatic stellate cell through targeting CRK.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with CRK elisa kit

 Reference Product  PubMed Publications
 Src elisa kit  >610 publications with CRK and Src
 Bcar1 elisa kit  >368 publications with CRK and Bcar1
 Ptk2 elisa kit  >151 publications with CRK and Ptk2
 Cbl elisa kit  >106 publications with CRK and Cbl
 Pxn elisa kit  >101 publications with CRK and Pxn
 Irs1 elisa kit  >12 publications with CRK and Irs1
 Frs2 elisa kit  >4 publications with CRK and Frs2
 Rapgef1 elisa kit  >2 publications with CRK and Rapgef1

Pathways associated with CRK elisa kit

 Products by Pathway  Pathway Diagram
 ARMS-mediated Activation Pathway antibodies  ARMS-mediated Activation Pathway Diagram
 B Cell Receptor Signaling Pathway antibodies  B Cell Receptor Signaling Pathway Diagram
 Bacterial Invasion Of Epithelial Cells Pathway antibodies  Bacterial Invasion Of Epithelial Cells Pathway Diagram
 Bacterial Invasion Of Epithelial Cells Pathway antibodies  Bacterial Invasion Of Epithelial Cells Pathway Diagram
 Chemokine Signaling Pathway antibodies  Chemokine Signaling Pathway Diagram
 Chemokine Signaling Pathway antibodies  Chemokine Signaling Pathway Diagram
 Chronic Myeloid Leukemia Pathway antibodies  Chronic Myeloid Leukemia Pathway Diagram
 Chronic Myeloid Leukemia Pathway antibodies  Chronic Myeloid Leukemia Pathway Diagram
 Cytokine Signaling In Immune System Pathway antibodies  Cytokine Signaling In Immune System Pathway Diagram
 Downstream Signal Transduction Pathway antibodies  Downstream Signal Transduction Pathway Diagram

Diseases associated with CRK elisa kit

 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >342 publications with CRK and Neoplasms
 Lung Neoplasms Antibodies  >39 publications with CRK and Lung Neoplasms
 Lung Diseases Antibodies  >33 publications with CRK and Lung Diseases
 Disease Models, Animal Antibodies  >31 publications with CRK and Disease Models, Animal
 Cardiovascular Diseases Antibodies  >25 publications with CRK and Cardiovascular Diseases
 Inflammation Antibodies  >17 publications with CRK and Inflammation
 Brain Diseases Antibodies  >15 publications with CRK and Brain Diseases
 Drug Toxicity Antibodies  >15 publications with CRK and Drug Toxicity
 Heart Diseases Antibodies  >14 publications with CRK and Heart Diseases
 Kidney Diseases Antibodies  >10 publications with CRK and Kidney Diseases

Organs/Tissues associated with CRK elisa kit

 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >79 publications with CRK and Brain
 Lung Antibodies  >63 publications with CRK and Lung
 Kidney Antibodies  >39 publications with CRK and Kidney
 Embryonic Tissue Antibodies  >32 publications with CRK and Embryonic Tissue
 Heart Antibodies  >31 publications with CRK and Heart
 Prostate Antibodies  >21 publications with CRK and Prostate
 Liver Antibodies  >20 publications with CRK and Liver
 Pancreas Antibodies  >13 publications with CRK and Pancreas
 Spleen Antibodies  >9 publications with CRK and Spleen
 Thymus Antibodies  >7 publications with CRK and Thymus
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