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Dopamine Beta-Hydroxylase (DBH) ELISA Kit for Human

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS285557

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate DBH in samples. An antibody specific for DBH has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyDBH present is bound by the… More details

$545 / 48 Tests +1 FREE 8GB USB

Product Documentation

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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Dopamine beta-hydroxylase (DBH), ELISA Kit
Full Product Name
Human Dopamine beta-hydroxylase (DBH) ELISA Kit
Product Gene Name
DBH elisa kit
Product Synonym Names
RP11-317B10.1; DBM; dopamine beta hydroxylase
Species Reactivity
Human
Assay Type
Sandwich
Samples
Serum, Plasma, Other biological fluids
Specificity
This assay has high sensitivity and excellent specificity for detection of Human DBH. No significant cross-reactivity or interference between Human DBH and analogues was observed.
Preparation and Storage
Store at 2-8 degree C.
Molecular Weight
69,065 Da
OMIM
AL365494 Genomic DNA
3D Structure
ModBase 3D Structure for P09172
Sample Volume
1-200 uL
Precision
Intra-assay Precision (Precision within an assay)
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%) = SD/meanX100
Intra-Assay: CV
Inter-Assay: CV
Detection Wavelength
450 nm
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.

Other Notes

Small volumes of DBH elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for DBH purchase

MBS285557 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Dopamine beta-hydroxylase (DBH) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing DBH. The ELISA analytical biochemical technique of the MBS285557 kit is based on DBH antibody-DBH antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect DBH antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, DBH. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for DBH. It may not necessarily be applicable to this product.

NCBI GI #
116534900
NCBI GeneID
1621
NCBI Accession #
NP_000778.3
UniProt Primary Accession #
P09172
UniProt Secondary Accession #
Q5T381; Q96AG2
UniProt Related Accession #
P09172
GenBank Nucleotide #
NM_000787.3
NCBI Official Full Name
dopamine beta-hydroxylase
NCBI Official Synonym Full Names
dopamine beta-hydroxylase
NCBI Official Symbol
DBH
NCBI Official Synonym Symbols
DBM
UniProt Protein Name
Dopamine beta-hydroxylase
UniProt Synonym Protein Names
Dopamine beta-monooxygenase
Protein Family
Dopamine beta-hydroxylase
UniProt Gene Name
DBH
NCBI Protein Information
dopamine beta-hydroxylase

NCBI Summary for DBH

The protein encoded by this gene is an oxidoreductase belonging to the copper type II, ascorbate-dependent monooxygenase family. The encoded protein, expressed in neuroscretory vesicles and chromaffin granules of the adrenal medulla, catalyzes the conversion of dopamine to norepinephrine, which functions as both a hormone and as the main neurotransmitter of the sympathetic nervous system. The enzyme encoded by this gene exists exists in both soluble and membrane-bound forms, depending on the absence or presence, respectively, of a signal peptide. Mutations in this gene cause dopamine beta-hydroxylate deficiency in human patients, characterized by deficits in autonomic and cardiovascular function, including hypotension and ptosis. Polymorphisms in this gene may play a role in a variety of psychiatric disorders. [provided by RefSeq, Aug 2017]
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UniProt Comments for DBH

DBH: Conversion of dopamine to noradrenaline. Homotetramer composed of two non-covalently bound disulfide-linked dimers. Activity is enhanced by nerve growth factor (in superior cervical ganglia and adrenal medulla). Trans-synaptic stimulation with reserpine, acetylcholine and glucocorticoids. Belongs to the copper type II ascorbate-dependent monooxygenase family.

Protein type: Amino Acid Metabolism - tyrosine; Apoptosis; Cell cycle regulation; EC 1.14.17.1; Membrane protein, integral; Motility/polarity/chemotaxis; Oxidoreductase

Chromosomal Location of Human Ortholog: 9q34.2

Cellular Component: cytoplasm; extracellular region; extracellular space; integral component of membrane; intracellular membrane-bound organelle; membrane; secretory granule membrane

Molecular Function: catalytic activity; copper ion binding; dopamine beta-monooxygenase activity; L-ascorbic acid binding

Biological Process: behavioral response to ethanol; blood vessel remodeling; catecholamine biosynthetic process; cytokine production; dopamine catabolic process; fear response; glucose homeostasis; homoiothermy; leukocyte mediated immunity; leukocyte migration; locomotory behavior; maternal behavior; memory; norepinephrine biosynthetic process; octopamine biosynthetic process; positive regulation of vasoconstriction; regulation of cell proliferation; response to amphetamine; response to pain; synaptic transmission; visual learning

Disease: Dopamine Beta-hydroxylase Deficiency, Congenital
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Research Articles on DBH

1. This study indicated that DBH5'-Ins/Del polymorphism may not play a role in the susceptibility to tardive dyskinesia and cognitive deficits in schizophrenia with tardive dyskinesia.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with DBH elisa kit

 Reference Product  PubMed Publications
 PNMT elisa kit  >191 publications with DBH and PNMT
 COMT elisa kit  >87 publications with DBH and COMT
 DDC elisa kit  >41 publications with DBH and DDC
 PHOX2B elisa kit  >35 publications with DBH and PHOX2B
 MAOA elisa kit  >28 publications with DBH and MAOA
 MAOB elisa kit  >6 publications with DBH and MAOB
 TRIM11 elisa kit  >1 publications with DBH and TRIM11

Pathways associated with DBH elisa kit

 Products by Pathway  Pathway Diagram
 Amine-derived Hormones Pathway antibodies  Amine-derived Hormones Pathway Diagram
 Biogenic Amine Synthesis Pathway antibodies  Biogenic Amine Synthesis Pathway Diagram
 Catecholamine Biosynthesis Pathway antibodies  Catecholamine Biosynthesis Pathway Diagram
 Catecholamine Biosynthesis, Tyrosine => Dopamine => Noradrenaline => Adrenaline Pathway antibodies  Catecholamine Biosynthesis, Tyrosine => Dopamine => Noradrenaline => Adrenaline Pathway Diagram
 Catecholamine Biosynthesis, Tyrosine => Dopamine => Noradrenaline => Adrenaline Pathway antibodies  Catecholamine Biosynthesis, Tyrosine => Dopamine => Noradrenaline => Adrenaline Pathway Diagram
 Metabolic Pathways antibodies  Metabolic Pathways Diagram
 Metabolism Pathway antibodies  Metabolism Pathway Diagram
 Metabolism Of Amino Acids And Derivatives Pathway antibodies  Metabolism Of Amino Acids And Derivatives Pathway Diagram
 Monoamine Transport Pathway antibodies  Monoamine Transport Pathway Diagram
 Tyrosine Metabolism Pathway antibodies  Tyrosine Metabolism Pathway Diagram

Diseases associated with DBH elisa kit

 Disease Name  Pubmed Publications
 Mental Disorders Antibodies  >266 publications with DBH and Mental Disorders
 Nervous System Diseases Antibodies  >226 publications with DBH and Nervous System Diseases
 Hypertension Antibodies  >95 publications with DBH and Hypertension
 Movement Disorders Antibodies  >62 publications with DBH and Movement Disorders
 Neuroblastoma Antibodies  >48 publications with DBH and Neuroblastoma
 Hypotension Antibodies  >43 publications with DBH and Hypotension
 Pheochromocytoma Antibodies  >41 publications with DBH and Pheochromocytoma
 Depressive Disorder Antibodies  >39 publications with DBH and Depressive Disorder
 Parkinsonian Disorders Antibodies  >30 publications with DBH and Parkinsonian Disorders
 Bipolar Disorder Antibodies  >29 publications with DBH and Bipolar Disorder

Organs/Tissues associated with DBH elisa kit

 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >627 publications with DBH and Brain
 Nerve Antibodies  >388 publications with DBH and Nerve
 Adrenal Gland Antibodies  >223 publications with DBH and Adrenal Gland
 Ganglia Antibodies  >162 publications with DBH and Ganglia
 Embryonic Tissue Antibodies  >29 publications with DBH and Embryonic Tissue
 Mouth Antibodies  >16 publications with DBH and Mouth
 Testis Antibodies  >7 publications with DBH and Testis
 Lymph Node Antibodies  >5 publications with DBH and Lymph Node
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