Intermediate Conductance Calcium-Activated Potassium Channel Protein 4 (KCNN4) ELISA Kit for Human

Name: Human KCNN4 ELISA Kit; MyBioSource
Brand: MyBioSource
SKU: MBS285600
Price: 495 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS285600

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate KCNN4 in samples. An antibody specific for KCNN4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyKCNN4 present is bound by the… More details

$495 / 48 Tests +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Request Current Manual

Product Overview

Online Datasheet in PDF

Product Name

Intermediate conductance calcium-activated potassium channel protein 4 (KCNN4), ELISA Kit

Full Product Name

Human Intermediate conductance calcium-activated potassium channel protein 4 (KCNN4) ELISA Kit

Product Gene Name

KCNN4 elisa kit

Product Synonym Names

IK1; IKCA1; KCA4; KCa3.1; SK4; hIKCa1; hKCa4; hSK4; intermediate conductance calcium-activated potassium channel protein 1|putative erythrocyte intermediate conductance calcium-activated potassium G

Species Reactivity

Human

Assay Type

Sandwich

Samples

Serum, Plasma, Other biological fluids

Specificity

This assay has high sensitivity and excellent specificity for detection of Human KCNN4. No significant cross-reactivity or interference between Human KCNN4 and analogues was observed.

Preparation and Storage

Store at 2-8 degree C.

Molecular Weight

47,696 Da

OMIM

AF000972 mRNA

3D Structure

ModBase 3D Structure for O15554

Sample Volume

1-200 uL

Precision

Intra-assay Precision (Precision within an assay)
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%) = SD/meanX100
Intra-Assay: CV
Inter-Assay: CV

Detection Wavelength

450 nm

ISO Certification

Manufactured in an ISO 9001:2015 Certified Laboratory.

Other Notes

Small volumes of KCNN4 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for KCNN4 purchase

MBS285600 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Intermediate conductance calcium-activated potassium channel protein 4 (KCNN4) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing KCNN4. The ELISA analytical biochemical technique of the MBS285600 kit is based on KCNN4 antibody-KCNN4 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect KCNN4 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, KCNN4. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for KCNN4. It may not necessarily be applicable to this product.

NCBI GI #

4504859

NCBI GeneID

3783

NCBI Accession #

NP_002241.1

UniProt Primary Accession #

O15554

UniProt Secondary Accession #

Q53XR4

UniProt Related Accession #

O15554

GenBank Nucleotide #

NM_002250.2

NCBI Official Full Name

intermediate conductance calcium-activated potassium channel protein 4

NCBI Official Synonym Full Names

potassium calcium-activated channel subfamily N member 4

NCBI Official Symbol

KCNN4

NCBI Official Synonym Symbols

IK; IK1; SK4; DHS2; KCA4; hSK4; IKCA1; hKCa4; KCa3.1; hIKCa1

UniProt Protein Name

Intermediate conductance calcium-activated potassium channel protein 4

UniProt Synonym Protein Names

IKCa1; IK1; KCa3.1; KCa4; Putative Gardos channel

Protein Family

Intermediate conductance calcium-activated potassium channel protein

UniProt Gene Name

KCNN4

UniProt Synonym Gene Names

IK1; IKCA1; KCA4; SK4; SK4; SKCa 4; SKCa4; IK1

NCBI Protein Information

intermediate conductance calcium-activated potassium channel protein 4

NCBI Summary for KCNN4

The protein encoded by this gene is part of a potentially heterotetrameric voltage-independent potassium channel that is activated by intracellular calcium. Activation is followed by membrane hyperpolarization, which promotes calcium influx. The encoded protein may be part of the predominant calcium-activated potassium channel in T-lymphocytes. This gene is similar to other KCNN family potassium channel genes, but it differs enough to possibly be considered as part of a new subfamily. [provided by RefSeq, Jul 2008]

UniProt Comments for KCNN4

KCNN4: Forms a voltage-independent potassium channel that is activated by intracellular calcium. Activation is followed by membrane hyperpolarization which promotes calcium influx. Required for maximal calcium influx and proliferation during the reactivation of naive T-cells. The channel is blocked by clotrimazole and charybdotoxin but is insensitive to apamin. Belongs to the potassium channel KCNN family. KCa3.1/KCNN4 subfamily.

Protein type: Channel, potassium; Membrane protein, integral; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 19q13.31

Cellular Component: cell soma; plasma membrane; vesicle; voltage-gated potassium channel complex

Molecular Function: calcium-activated potassium channel activity; calmodulin binding; potassium channel activity; protein binding; protein phosphatase binding; small conductance calcium-activated potassium channel activity

Biological Process: calcium ion transport; cell volume homeostasis; defense response; immune system process; phospholipid translocation; positive regulation of protein secretion; positive regulation of T cell receptor signaling pathway; potassium ion transmembrane transport; potassium ion transport; saliva secretion; stabilization of membrane potential

Disease: Dehydrated Hereditary Stomatocytosis 2

Research Articles on KCNN4

1. Human arrhythmogenic calmodulin mutations impede the activation of SK2 channels in human embryonic kidney 293 cells.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with KCNN4 elisa kit

Reference Product	PubMed Publications
PRKAG1 elisa kit	>1 publications with KCNN4 and PRKAG1

Pathways associated with KCNN4 elisa kit

Products by Pathway	Pathway Diagram
Ca2+ Activated K+ Channels Pathway antibodies	Ca2+ Activated K+ Channels Pathway Diagram
Insulin Secretion Pathway antibodies	Insulin Secretion Pathway Diagram
Neuronal System Pathway antibodies	Neuronal System Pathway Diagram
Potassium Channels Pathway antibodies	Potassium Channels Pathway Diagram
Protein Digestion And Absorption Pathway antibodies	Protein Digestion And Absorption Pathway Diagram
Protein Digestion And Absorption Pathway antibodies	Protein Digestion And Absorption Pathway Diagram
Salivary Secretion Pathway antibodies	Salivary Secretion Pathway Diagram
Salivary Secretion Pathway antibodies	Salivary Secretion Pathway Diagram

Diseases associated with KCNN4 elisa kit

Disease Name	Pubmed Publications
Neoplasms Antibodies	>22 publications with KCNN4 and Neoplasms
Disease Models, Animal Antibodies	>20 publications with KCNN4 and Disease Models, Animal
Cardiovascular Diseases Antibodies	>18 publications with KCNN4 and Cardiovascular Diseases
Inflammation Antibodies	>17 publications with KCNN4 and Inflammation
Stroke Antibodies	>7 publications with KCNN4 and Stroke
Atherosclerosis Antibodies	>6 publications with KCNN4 and Atherosclerosis
Heart Diseases Antibodies	>5 publications with KCNN4 and Heart Diseases
Carcinoma Antibodies	>5 publications with KCNN4 and Carcinoma
Hypertension Antibodies	>5 publications with KCNN4 and Hypertension
Drug Toxicity Antibodies	>5 publications with KCNN4 and Drug Toxicity

Organs/Tissues associated with KCNN4 elisa kit

Organ/Tissue Name	Pubmed Publications
Blood Antibodies	>93 publications with KCNN4 and Blood
Intestine Antibodies	>27 publications with KCNN4 and Intestine
Brain Antibodies	>25 publications with KCNN4 and Brain
Lung Antibodies	>21 publications with KCNN4 and Lung
Liver Antibodies	>13 publications with KCNN4 and Liver
Bone Antibodies	>9 publications with KCNN4 and Bone
Pancreas Antibodies	>8 publications with KCNN4 and Pancreas
Bone Marrow Antibodies	>8 publications with KCNN4 and Bone Marrow
Ovary Antibodies	>5 publications with KCNN4 and Ovary
Embryonic Tissue Antibodies	>5 publications with KCNN4 and Embryonic Tissue