Ropporin-1-Like (ROPN1L) ELISA Kit for Human

Name: Human ROPN1L ELISA Kit; Ropporin-1-Like; MyBioSource
Brand: MyBioSource
SKU: MBS285608
Price: 495 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS285608

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate ROPN1L in samples. An antibody specific for ROPN1L has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyROPN1L present is bound by… More details

$495 / 48 Tests +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

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Product Overview

Online Datasheet in PDF

Product Name

Ropporin-1-like (ROPN1L), ELISA Kit

Full Product Name

Human Ropporin-1-like protein (ROPN1L) ELISA Kit

Product Gene Name

ROPN1L elisa kit

Product Synonym Names

ASP; FLJ23003; FLJ25776; RSPH11; AKAP-associated sperm protein|radial spoke head 11 homolog

Species Reactivity

Human

Assay Type

Sandwich

Samples

Serum, Plasma, Other biological fluids

Specificity

This assay has high sensitivity and excellent specificity for detection of Human ROPN1L. No significant cross-reactivity or interference between Human ROPN1L and analogues was observed.

Preparation and Storage

Store at 2-8 degree C.

Molecular Weight

26,107 Da

OMIM

AF239723 mRNA

3D Structure

ModBase 3D Structure for Q96C74

Sample Volume

1-200 uL

Precision

Intra-assay Precision (Precision within an assay)
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%) = SD/meanX100
Intra-Assay: CV
Inter-Assay: CV

Detection Wavelength

450 nm

ISO Certification

Manufactured in an ISO 9001:2015 Certified Laboratory.

Other Notes

Small volumes of ROPN1L elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for ROPN1L purchase

MBS285608 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Ropporin-1-like (ROPN1L) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing ROPN1L. The ELISA analytical biochemical technique of the MBS285608 kit is based on ROPN1L antibody-ROPN1L antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect ROPN1L antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, ROPN1L. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for ROPN1L. It may not necessarily be applicable to this product.

NCBI GI #

319890246

NCBI GeneID

83853

NCBI Accession #

NP_001188395.1

UniProt Primary Accession #

Q96C74

UniProt Secondary Accession #

Q9BZX0; D3DTC9

UniProt Related Accession #

Q96C74

GenBank Nucleotide #

NM_001201466.1

NCBI Official Full Name

ropporin-1-like protein

NCBI Official Synonym Full Names

rhophilin associated tail protein 1 like

NCBI Official Symbol

ROPN1L

NCBI Official Synonym Symbols

ASP; RSPH11

UniProt Protein Name

Ropporin-1-like protein

UniProt Synonym Protein Names

AKAP-associated sperm protein

Protein Family

Ropporin-1-like protein

UniProt Gene Name

ROPN1L

UniProt Synonym Gene Names

ASP; ROPN1-like protein

NCBI Protein Information

ropporin-1-like protein

NCBI Summary for ROPN1L

This gene encodes a member of the ropporin family. The encoded protein is present in sperm and interacts with A-kinase anchoring protein, AKAP3, through the amphipathic helix region of AKAP3. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Oct 2014]

UniProt Comments for ROPN1L

ROPN1L: a member of the ropporin family. The encoded protein is a sperm protein. It interacts with A-kinase anchoring protein, AKAP3, through the amphipathic helix region of AKAP3. Type II regulatory subunit of cAMP-dependent protein kinase (PKARII) also binds to this helix domain of AKAP3, which allows PKARII to be targeted to specific subcellular compartments. It is suggested that sperm contains several proteins that bind to AKAPs in a manner similar to PKARII, and this encoded protein may be one of them. Alternatively spliced transcript variants encoding the same protein have been found for this gene.[provided by RefSeq, Jan 2011]

Protein type: Protein kinase, regulatory subunit

Chromosomal Location of Human Ortholog: 5p15.2

Cellular Component: cytoplasm

Molecular Function: protein binding

Biological Process: regulation of protein amino acid phosphorylation; sperm capacitation; sperm motility

Research Articles on ROPN1L

1. Sp17 and ASP cancer/testis antigens were found in ciliated cells of four ciliated hepatic foregut cysts (CHFCs). Further characterization of Sp17 and ASP in patients with CHFCs may provide significant clues for understanding the molecular mechanisms underlying their predisposition to develop squamous cell carcinomas.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with ROPN1L elisa kit

Reference Product	PubMed Publications
ST3GAL6 elisa kit	>1 publications with ROPN1L and ST3GAL6
PPM1L elisa kit	>1 publications with ROPN1L and PPM1L
SLC37A3 elisa kit	>1 publications with ROPN1L and SLC37A3
SPA17 elisa kit	>1 publications with ROPN1L and SPA17
CABYR elisa kit	>1 publications with ROPN1L and CABYR
AKAP3 elisa kit	>1 publications with ROPN1L and AKAP3

Diseases associated with ROPN1L elisa kit

Disease Name	Pubmed Publications
Breast Neoplasms Antibodies	>2 publications with ROPN1L and Breast Neoplasms
Infertility, Male Antibodies	>1 publications with ROPN1L and Infertility, Male

Organs/Tissues associated with ROPN1L elisa kit

Organ/Tissue Name	Pubmed Publications
Testis Antibodies	>3 publications with ROPN1L and Testis
Kidney Antibodies	>1 publications with ROPN1L and Kidney
Lung Antibodies	>1 publications with ROPN1L and Lung
Brain Antibodies	>1 publications with ROPN1L and Brain